scholarly journals Vasoactive intestinal polypeptide-induced chloride secretion by a colonic epithelial cell line. Direct participation of a basolaterally localized Na+,K+,Cl- cotransport system.

1985 ◽  
Vol 75 (2) ◽  
pp. 462-471 ◽  
Author(s):  
K Dharmsathaphorn ◽  
K G Mandel ◽  
H Masui ◽  
J A McRoberts
1989 ◽  
Vol 256 (1) ◽  
pp. C197-C203 ◽  
Author(s):  
K. E. Barrett ◽  
P. A. Huott ◽  
S. S. Shah ◽  
K. Dharmsathaphorn ◽  
S. I. Wasserman

Adenosine and its agonists, 5'-(N-ethylcarboxamido)adenosine and N6-(D-2-phenylisopropyl)-adenosine, induced a sustained increase in chloride secretion when added to either the apical or basolateral aspect of monolayers of the human colonic epithelial cell line T84. Secretion was induced with identical kinetics by addition to both sides, but apical addition was less potent. The rank order of potency of the agonists on either side was consistent with the presence of an adenosine A2-receptor, but the apical and basolateral receptors differed in both their ability to stimulate increases in adenosine 3',5'-cyclic monophosphate, and their susceptibility to down-modulation by chronic exposure to 5'-(N-ethylcarboxamido) adenosine in culture.


2019 ◽  
Vol 16 (1) ◽  
pp. 135-144 ◽  
Author(s):  
Rohit Gundamaraju ◽  
Ravichandra Vemuri ◽  
Wai Chin Chong ◽  
Andrew Cameron Bulmer ◽  
Rajaraman Eri

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