scholarly journals An Improved Continuous Flow Method for Serum Creatinine Using the Jaffé Reaction

1980 ◽  
Vol 17 (3) ◽  
pp. 153-154 ◽  
Author(s):  
W A White ◽  
E C Attwood
Keyword(s):  
Serum Creatinine ◽  
Continuous Flow ◽  
First Generation ◽  
Flow Method ◽  
Baseline Noise ◽  
Continuous Flow Method

A method for the measurement of creatinine is described in which serum is dialysed directly into a combined alkaline picrate reagent. Baseline noise and drift are eliminated, and precision is improved by avoiding the need to add further reagents after dialysis. This may be of particular interest to laboratories that perform this assay on first-generation AutoAnalyzer equipment.

A stopped-flow/continuous-flow method for kinetic determinations

1995 ◽  
Vol 309 (1-3) ◽  
pp. 277-282 ◽  
Author(s):  
Yun-Sheng Hsieh ◽  
S.R. Crouch
Keyword(s):  
Continuous Flow ◽  
Stopped Flow ◽  
Flow Method ◽  
Continuous Flow Method

ZnO-Based Catalyst for Photodegradation of 2-Chlorophenol in Aqueous Solution Under Simulated Solar Light Using a Continuous Flow Method

JOM ◽  
2020 ◽  
Vol 73 (1) ◽  
pp. 404-410
Author(s):  
Ahed H. Zyoud ◽  
Hala Salah ◽  
Shaher H. Zyoud ◽  
Samer H. Zyoud ◽  
Muath H. Helal ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Continuous Flow ◽  
Solar Light ◽  
Flow Method ◽  
Simulated Solar Light ◽  
Continuous Flow Method

Continuous-flow fluorometry of low galactose concentrations in blood or plasma.

1980 ◽  
Vol 26 (2) ◽  
pp. 282-285 ◽  
Author(s):  
J M Henderson ◽  
F W Fales
Keyword(s):  
Blood Flow ◽  
Hepatic Blood Flow ◽  
Continuous Flow ◽  
Normal Subjects ◽  
Galactose Oxidase ◽  
Fluorogenic Substrate ◽  
Flow Method ◽  
Peroxidase Reaction ◽  
Hydroxyphenylacetic Acid ◽  
Continuous Flow Method

Abstract Clearance of 0-100 mg/L concentrations of galactose from the blood depends on nutrient hepatic blood flow. We can measure such concentrations, which was not previously possible, by a continuous-flow method involving the use of galactose oxidase and peroxidase, the latter being coupled to a fluorogenic substrate, p-hydroxyphenylacetic acid. Interfering substances in the peroxidase reaction are removed by zinc/alkali precipitation. Sensitivity is maximized by using saturating concentrations of the enzymes and substrate. In prepared plasma test samples with galactose concentrations of 10, 40, 70, and 100 mg/L, the within-run CV's ranged from 2.1 to 8.6%, and day-to-day CV's from 2.2 to 17.2%, the largest CV's being for the 10 mg/L concentration. Normal subjects are shown to clear galactose more efficiently than subjects with moderate cirrhosis.

Improvements in and clinical utility of a continuous-flow method for routine measurement of dialyzable (ultrafiltrable) calcium.

1979 ◽  
Vol 25 (11) ◽  
pp. 1939-1943 ◽  
Author(s):  
J Toffaletti ◽  
G N Bowers
Keyword(s):  
Continuous Flow ◽  
Clinical Utility ◽  
Clinical Laboratory ◽  
Serum Samples ◽  
The Past ◽  
Baseline Noise ◽  
Routine Service ◽  
Routine Measurement ◽  
Routine Clinical Laboratory ◽  
Continuous Flow Method

Abstract We describe modifications to the original continuous-flow procedure for dialyzable calcium (Clin. Chem. 23: 1258, 1977) needed to make the method more suitable for routine clinical laboratory use. The modifications simplify the continuous-flow (AutoAnalyzer) manifold, decrease baseline noise, increase the sensitivity, and permit use of a less-expensive fluorometer. Bias due to variation in serum processing is minimized by use of serum samples minimally exposed to air and a pH 7.40 buffer in place of the routinely processed sera and pH 7.30 buffer used formerly. Day-to-day precision (CV) during the past year for samples that included three different lots of quality-control sera was 2 to 3%. The analysis requires 200 micro L of serum, collected with minor additional precautions. We find that dialyzable calcium can be dependably measured in the routine service laboratory and show how this information is clinically more useful than is information on total calcium in serum.

Sign in / Sign up

Export Citation Format

Share Document