Effects of Recombinant Dentin Sialoprotein in Dental Pulp Cells

Dentin sialophosphoprotein (DSPP) is critical for dentin mineralization. However, the function of dentin sialoprotein (DSP), the cleaved product of DSPP, remains unclear. This study aimed to investigate the signal transduction pathways and effects of recombinant human DSP (rh-DSP) on proliferation, migration, and odontoblastic differentiation in human dental pulp cells (HDPCs). The exogenous addition of rh-DSP enhanced the proliferation and migration of HDPCs in dose- and time-dependent manners. rh-DSP markedly increased ALP activity, calcium nodule formation, and levels of odontoblastic marker mRNA. rh-DSP increased BMP-2 expression and Smad1/5/8 phosphorylation, which was blocked by the BMP antagonist, noggin. Furthermore, rh-DSP phosphorylated extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), Akt, and IκB-α, and induced the nuclear translocation of the NF-κB p65 subunit. Analysis of these data demonstrates a novel signaling function of rh-DSP for the promotion of growth, migration, and differentiation in HDPCS via the BMP/Smad, JNK, ERK, MAPK, and NF-κB signaling pathways, suggesting that rh-DSP may have therapeutic utility in dentin regeneration or dental pulp tissue engineering.

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Expression and purification recombinant human dentin sialoprotein in Escherichia coli and its effects on human dental pulp cells

Protein Expression and Purification ◽

10.1016/j.pep.2012.03.005 ◽

2012 ◽

Vol 83 (1) ◽

pp. 47-51 ◽

Cited By ~ 7

Author(s):

Ye-Rang Yun ◽

Hae-Won Kim ◽

Wonmo Kang ◽

Eunyi Jeon ◽

Sujin Lee ◽

...

Keyword(s):

Escherichia Coli ◽

Dental Pulp ◽

Dental Pulp Cells ◽

Expression And Purification ◽

Human Dental Pulp Cells ◽

Human Dentin ◽

Human Dental Pulp ◽

Pulp Cells ◽

Dentin Sialoprotein

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Alcohol Inhibits Odontogenic Differentiation of Human Dental Pulp Cells by Activating mTOR Signaling

Stem Cells International ◽

10.1155/2017/8717454 ◽

2017 ◽

Vol 2017 ◽

pp. 1-10 ◽

Cited By ~ 4

Author(s):

Wei Qin ◽

Qi-Ting Huang ◽

Michael D. Weir ◽

Zhi Song ◽

Ashraf F. Fouad ◽

...

Keyword(s):

Behavioral Problems ◽

Dental Pulp ◽

Nodule Formation ◽

Dependent Manner ◽

Dental Pulp Cells ◽

Human Dental Pulp Cells ◽

Odontoblastic Differentiation ◽

Heavy Alcohol Consumption ◽

Human Dental Pulp ◽

Pulp Cells

Long-term heavy alcohol consumption could result in a range of health, social, and behavioral problems. People who abuse alcohol are at high risks of seriously having osteopenia, periodontal disease, and compromised oral health. However, the role of ethanol (EtOH) in the biological functions of human dental pulp cells (DPCs) is unknown. Whether EtOH affects the odontoblastic differentiation of DPCs through the mechanistic target of rapamycin (mTOR) remains unexplored. The objective of this study was to investigate the effects of EtOH on DPC differentiation and mineralization. DPCs were isolated and purified from human dental pulps. The proliferation and odontoblastic differentiation of DPCs treated with EtOH were subsequently investigated. Different doses of EtOH were shown to be cytocompatible with DPCs. EtOH significantly activated the mTOR pathway in a dose-dependent manner. In addition, EtOH downregulated the alkaline phosphatase activity, attenuated the mineralized nodule formation, and suppressed the expression of odontoblastic markers including ALP, DSPP, DMP-1, Runx2, and OCN. Moreover, the pretreatment with rapamycin, a specific mTOR inhibitor, markedly reversed the EtOH-induced odontoblastic differentiation and cell mineralization. Our findings show for the first time that EtOH can suppress DPC differentiation and mineralization in a mTOR-dependent manner, indicating that EtOH may be involved in negatively regulating the dental pulp repair.

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Combined effects of mineral trioxide aggregate and human placental extract on rat pulp tissue and growth, differentiation and angiogenesis in human dental pulp cells

Acta Odontologica Scandinavica ◽

10.3109/00016357.2015.1120882 ◽

2016 ◽

Vol 74 (4) ◽

pp. 298-306 ◽

Cited By ~ 8

Author(s):

Seok-Woo Chang ◽

Ji-Youn Kim ◽

Mi-Joo Kim ◽

Ga-Hyun Kim ◽

Jin-Kyu Yi ◽

...

Keyword(s):

Dental Pulp ◽

Mineral Trioxide Aggregate ◽

Combined Effects ◽

Dental Pulp Cells ◽

Pulp Tissue ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells ◽

Placental Extract

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The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽

10.3390/ma14092449 ◽

2021 ◽

Vol 14 (9) ◽

pp. 2449

Author(s):

Beata Kaczmarek-Szczepańska ◽

Olha Mazur ◽

Marta Michalska-Sionkowska ◽

Krzysztof Łukowicz ◽

Anna Maria Osyczka

Keyword(s):

Thermal Stability ◽

Tannic Acid ◽

Dental Pulp ◽

Blood Compatibility ◽

Dental Pulp Cells ◽

Preliminary Assessment ◽

Human Dental Pulp Cells ◽

Human Dental Pulp ◽

Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

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