Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

2021 ◽  
pp. 002203452110291
Author(s):  
M.M. Hasani-Sadrabadi ◽  
S. Pouraghaei ◽  
E. Zahedi ◽  
P. Sarrion ◽  
M. Ishijima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sustained Release ◽  
Surface Treatment ◽  
Osteogenic Differentiation ◽  
Orthopedic Implants ◽  
Layer By Layer ◽  
Implant Surface ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Osseointegration of dental, craniofacial, and orthopedic implants is critical for their long-term success. Multifunctional surface treatment of implants was found to significantly improve cell adhesion and induce osteogenic differentiation of dental-derived stem cells in vitro. Moreover, local and sustained release of antibiotics via nanolayers from the surface of implants can present unparalleled therapeutic benefits in implant dentistry. Here, we present a layer-by-layer surface treatment of titanium implants capable of incorporating BMP-2–mimicking short peptides and gentamicin to improve their osseointegration and antibacterial features. Additionally, instead of conventional surface treatments, we employed polydopamine coating before layer-by-layer assembly to initiate the formation of the nanolayers on rough titanium surfaces. Cytocompatibility analysis demonstrated that modifying the titanium implant surface with layer-by-layer assembly did not have adverse effects on cellular viability. The implemented nanoscale coating provided sustained release of osteoinductive peptides with an antibacterial drug. The surface-functionalized implants showed successful osteogenic differentiation of periodontal ligament stem cells and antimicrobial activity in vitro and increased osseointegration in a rodent animal model 4 wk postsurgery as compared with untreated implants. Altogether, our in vitro and in vivo studies suggest that this approach can be extended to other dental and orthopedic implants since this surface functionalization showed improved osseointegration and an enhanced success rate.

scholarly journals Chitosan-miRNA functionalized microporous titanium oxide surfaces via a layer-by-layer approach with a sustained release profile for enhanced osteogenic activity

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kaimin Wu ◽  
Mengyuan Liu ◽  
Nan Li ◽  
Li Zhang ◽  
Fanhui Meng ◽  
...  
Keyword(s):  
Sustained Release ◽  
Osteogenic Differentiation ◽  
Sodium Hyaluronate ◽  
Release Profile ◽  
Titanium Implants ◽  
Layer By Layer ◽  
Matrix Mineralization ◽  
In Vitro Transfection

Abstract Background The biofunctionalization of titanium implants for high osteogenic ability is a promising approach for the development of advanced implants to promote osseointegration, especially in compromised bone conditions. In this study, polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach with a chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolytes on microarc-oxidized (MAO) Ti surfaces via silane-glutaraldehyde coupling. Methods Dynamic contact angle and scanning electron microscopy measurements were conducted to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. The in vitro transfection efficiency and the cytotoxicity were investigated after seeding mesenchymal stem cells (MSCs) on the CS-antimiR-138/HA PEM-functionalized microporous Ti surface. The in vitro osteogenic differentiation of the MSCs and the in vivo osseointegration were also evaluated. Results The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles were distributed evenly across the MAO surface. The miRNA loading increased with increasing bilayer number. More importantly, a sustained miRNA release was obtained over a timeframe of approximately 2 weeks. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced the osteogenic differentiation of MSCs in terms of enhanced alkaline phosphatase, collagen production and extracellular matrix mineralization. Substantially enhanced in vivo osseointegration was observed in the rat model. Conclusions The findings demonstrated that the novel CS-antimiR-138/HA PEM-functionalized microporous Ti implant exhibited sustained release of CS-antimiR-138, and notably enhanced the in vitro osteogenic differentiation of MSCs and in vivo osseointegration. This novel miRNA-functionalized Ti implant may be used in the clinical setting to allow for more effective and robust osseointegration.

The Effect of Layer-by-Layer Assembly Coating on the Proliferation and Differentiation of Neural Stem Cells

2015 ◽  
Vol 7 (5) ◽  
pp. 3018-3029 ◽  
Author(s):  
Wenyan Li ◽  
Teng Guan ◽  
Xiaosha Zhang ◽  
Ziyuan Wang ◽  
Meng Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Proliferation And Differentiation ◽  
Layer Assembly

scholarly journals Chitosan-miRNA Functionalized Microporous Titanium Oxide Surface via A Layer-by-layer Approach with a Sustained Release Profile for Enhanced Osteogenic Activity

2020 ◽  
Author(s):  
Kaimin Wu ◽  
Mengyuan Liu ◽  
Nan Li ◽  
Li Zhang ◽  
Fanhui Meng ◽  
...  
Keyword(s):  
Sustained Release ◽  
Osteogenic Differentiation ◽  
Sodium Hyaluronate ◽  
Release Profile ◽  
Titanium Implants ◽  
Oxide Surface ◽  
Layer By Layer ◽  
Matrix Mineralization

Abstract Background: Biofunctionalization of titanium implants for high osteogenic ability holds the hope for advanced implant of promoted osseointegration, especially in the compromised bone condition. In this study, using chitosan-miRNA (CS-miRNA) complex and sodium hyaluronate (HA) as the positively and negatively charged polyelectrolyte, the polyelectrolyte multilayers (PEMs) were fabricated using the layer-by-layer approach on the microarc oxidized (MAO) Ti surface via silane glutaraldehyde coupling. Methods: Dynamic contact angle and scanning electron microscopy were firstly analyzed to monitor the layer accumulation. RiboGreen was used to quantify the miRNA loading and release profile in phosphate-buffered saline. In vitro transfection efficiency and cytotoxicity were investigated after mesenchymal stem cells (MSCs) being seeded on the CS-antimiR-138/HA PEM functionalized microporous Ti surface. The in vitro osteogenic differentiation of MSCs and in vivo osseointegration were also inspected. Results: The surface wettability alternately changed during the formation of PEMs. The CS-miRNA nanoparticles distributed evenly along the MAO surface. The miRNA loading amount increased with the bilayer number increasing. More importantly, a sustained miRNA release of over approximately 2 week was obtained. In vitro transfection revealed that the CS-antimiR-138 nanoparticles were taken up efficiently by the cells and caused significant knockdown of miR-138 without showing significant cytotoxicity. The CS-antimiR-138/HA PEM surface enhanced osteogenic differentiation of MSCs on it in terms of enhanced alkaline phosphatase, collagen product and extracellular matrix mineralization. In the rat model, it led to dramatically enhanced in vivo osseointegration. Conclusions: All these findings demonstrate that novel CS-antimiR-138/HA PEM functionalized microporous Ti implant exhibits sustained release of CS-antimiR-138, and obviously enhances the in vitro osteogenic differentiation of MSCs and dramatically enhanced in vivo osseointegration. This novel miRNA functionalized Ti implant may be used in clinic to allow more effective and robust osseointegration.

scholarly journals Bioactive Sphene-Based Ceramic Coatings on cpTi Substrates for Dental Implants: An In Vitro Study

Materials ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 2234 ◽  
Author(s):  
Hamada Elsayed ◽  
Giulia Brunello ◽  
Chiara Gardin ◽  
Letizia Ferroni ◽  
Denis Badocco ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
In Vitro Study ◽  
Titanium Implant ◽  
Orthopedic Implants ◽  
Ceramic Coatings ◽  
Immunofluorescent Staining ◽  
Implant Surface ◽  
Osteogenic Differentiation Medium ◽  
Alizarin Red

Titanium implant surface modifications have been widely investigated to favor the process of osseointegration. The present work aimed to evaluate the effect of sphene (CaTiSiO5) biocoating, on titanium substrates, on the in vitro osteogenic differentiation of Human Adipose-Derived Stem Cells (hADSCs). Sphene bioceramic coatings were prepared using preceramic polymers and nano-sized active fillers and deposited by spray coating. Scanning Electron Microscopy (SEM) analysis, surface roughness measurements and X-ray diffraction analysis were performed. The chemical stability of the coatings in Tris-HCl solution was investigated. In vitro studies were performed by means of proliferation test of hADSCs seeded on coated and uncoated samples after 21 days. Methyl Thiazolyl-Tetrazolium (MTT) test and immunofluorescent staining with phalloidin confirmed the in vitro biocompatibility of both substrates. In vitro osteogenic differentiation of the cells was evaluated using Alizarin Red S staining and quantification assay and real-time PCR (Polymerase Chain Reaction). When hADSCs were cultured in the presence of Osteogenic Differentiation Medium, a significantly higher accumulation of calcium deposits onto the sphene-coated surfaces than on uncoated controls was detected. Osteogenic differentiation on both samples was confirmed by PCR. The proposed coating seems to be promising for dental and orthopedic implants, in terms of composition and deposition technology.

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