Reduced in vivo Cell-mediated Immune Responses to Mumps, Tuberculin, and Streptokinase/Streptodornase but not to Candida albicans in Oral Lichen Planus

OBJECTIVE: Our goal was to analyze p63 and 14-3-3σ expression in normal and hyperdifferentiated head and neck mucosa. STUDY DESIGN: Compare the in vivo expression of p63 and 14-3-3σ by immunohistochemistry in normal mucosa and oral lichen planus, a benign mucosal lesion marked by hyperdifferentiation and apoptosis. RESULTS AND CONCLUSION: p63 is underexpressed and 14-3-3σ is overexpressed in lichen planus on immunohistochemical analysis. SIGNIFICANCE: The findings support the hypothesis that p63 plays an antidifferentiation role, whereas 14-3-3σ plays a prodifferentiation role in the upper aerodigestive tract epithelium. Lichen planus is a valuable model for the study of p63, 14-3-3σ, and mucosal differentiation. p63 and 14-3-3σ may be molecular markers for oral lichen planus.

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A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

10.21203/rs.2.14716/v1 ◽

2019 ◽

Author(s):

Hong He ◽

Mengyun Zhang ◽

Xinyu Xia ◽

Haiping Yang ◽

Qiao Peng

Keyword(s):

Candida Albicans ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Random Amplified Polymorphic Dna ◽

Type I ◽

Similarity Coefficients ◽

Professional Recognition ◽

Normal Individuals ◽

Polymerase Chain ◽

Oral Lichen

Abstract Objectives：This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods：Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results：The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions：The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.

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A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

10.21203/rs.2.14716/v4 ◽

2020 ◽

Author(s):

Hong He ◽

Xinyu Xia ◽

Haiping Yang ◽

Qiao Peng ◽

Jiaoer Zheng

Keyword(s):

Candida Albicans ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Random Amplified Polymorphic Dna ◽

Type I ◽

Similarity Coefficients ◽

Chain Reactions ◽

Polymerase Chain Reactions ◽

Normal Individuals ◽

Oral Lichen

Abstract Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

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Angiogenesis in Oral Lichen Planus: An In Vivo and Immunohistological Evaluation

Archivum Immunologiae et Therapiae Experimentalis ◽

10.1007/s00005-011-0150-1 ◽

2011 ◽

Vol 59 (6) ◽

pp. 457-462 ◽

Cited By ~ 11

Author(s):

Giuseppe Alessandro Scardina ◽

Alessia Ruggieri ◽

Emiliano Maresi ◽

Pietro Messina

Keyword(s):

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Lichen

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In-vivo usefulness of optical coherence tomography in atrophic-erosive oral lichen planus: Comparison between histopathological and ultrastructural findings

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2020.112009 ◽

2020 ◽

Vol 211 ◽

pp. 112009

Author(s):

Alessio Gambino ◽

Marco Cabras ◽

Adriana Cafaro ◽

Roberto Broccoletti ◽

Stefano Carossa ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Optical Coherence ◽

Oral Lichen ◽

Ultrastructural Findings

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Novel Bilayer Mucoadhesive Patches for Delivery of Clobetasol-17-Propionate to the Oral Mucosa to Treat Oral Lichen Planus; an in Vitro and in Vivo Evaluation

Oral Surgery Oral Medicine Oral Pathology and Oral Radiology ◽

10.1016/j.oooo.2018.05.037 ◽

2018 ◽

Vol 126 (4) ◽

pp. e204

Author(s):

Helen E. Colley ◽

Zulfahami Said ◽

Martin E. Santocildes-Romero ◽

Sarah R. Baker ◽

Katie D'Apice ◽

...

Keyword(s):

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

In Vivo Evaluation ◽

Oral Lichen

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Candida albicans induces TLR2/MyD88/NF-κB signaling and inflammation in oral lichen planus-derived keratinocytes

The Journal of Infection in Developing Countries ◽

10.3855/jidc.8062 ◽

2018 ◽

Vol 12 (09) ◽

pp. 780-786

Author(s):

Jianwei Liu ◽

Fanghui Geng ◽

Hongying Sun ◽

Xiaxia Wang ◽

Hui Zhang ◽

...

Keyword(s):

Candida Albicans ◽

Signaling Pathway ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Mucosal Disease ◽

Mrna And Protein Expression ◽

Key Events ◽

The Impact ◽

Oral Lichen ◽

Tlr2 Mrna

Introduction: The risk of oral lichen planus (OLP), a chronic inflammatory oral mucosal disease, becoming malignant increases by 21-fold in patients with fungal infection. This study examined the impact of Candida albicans exposure on Toll-like receptor (TLR) signaling in primary keratinocyte cultures obtained from OLP patients. Methodology: Following co-culture of primary OLP keratinocyte cultures with C. albicans for 24 hours, inflammatory cytokine concentrations were determined by ELISA. TLR2, MyD88, and NF-κBp65 mRNA and protein expression were assessed using quantitative RT-PCR and Western blot analyses, respectively. Keratinocyte apoptosis was also determined by flow cytometry. Results: IL-10, IL-8, IL-2, and TNF-ɑ levels were significantly higher following co-culture with C. albicans (all p ≤ 0.034). MyD88, NF-κB p65, and TLR2 mRNA (all p < 0.001) and protein (all p ≤ 0.004) expression levels were significantly higher in OLP keratinocytes following C. albicans exposure. Finally, the apoptosis rates of OLP keratinocytes were 21.2%, 29.4%, and 25.4% for the control cells and 3.9%, 5.6%, and 4.4% for those exposed to C. albicans, suggesting that co-culture with C. albicans inhibits the apoptosis of OLP keratinocytes. Conclusions: C. albicans activates the TLR2/MyD88/NF-κB signaling pathway in OLP keratinocytes, resulting in increased cytokine expression and decreased keratinocyte apoptosis. Two key events in the pathogenesis of OLP and its progression to malignancy, namely increased inflammation and decreased apoptosis, were induced by exposure to C. albicans. Thus, targeting this signaling pathway may represent a novel therapeutic strategy to prevent OLP malignant transformation.

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