scholarly journals Candida albicans induces TLR2/MyD88/NF-κB signaling and inflammation in oral lichen planus-derived keratinocytes

2018 ◽  
Vol 12 (09) ◽  
pp. 780-786
Author(s):  
Jianwei Liu ◽  
Fanghui Geng ◽  
Hongying Sun ◽  
Xiaxia Wang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Signaling Pathway ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Mucosal Disease ◽  
Mrna And Protein Expression ◽  
Key Events ◽  
The Impact ◽  
Oral Lichen ◽  
Tlr2 Mrna

Introduction: The risk of oral lichen planus (OLP), a chronic inflammatory oral mucosal disease, becoming malignant increases by 21-fold in patients with fungal infection. This study examined the impact of Candida albicans exposure on Toll-like receptor (TLR) signaling in primary keratinocyte cultures obtained from OLP patients. Methodology: Following co-culture of primary OLP keratinocyte cultures with C. albicans for 24 hours, inflammatory cytokine concentrations were determined by ELISA. TLR2, MyD88, and NF-κBp65 mRNA and protein expression were assessed using quantitative RT-PCR and Western blot analyses, respectively. Keratinocyte apoptosis was also determined by flow cytometry. Results: IL-10, IL-8, IL-2, and TNF-ɑ levels were significantly higher following co-culture with C. albicans (all p ≤ 0.034). MyD88, NF-κB p65, and TLR2 mRNA (all p < 0.001) and protein (all p ≤ 0.004) expression levels were significantly higher in OLP keratinocytes following C. albicans exposure. Finally, the apoptosis rates of OLP keratinocytes were 21.2%, 29.4%, and 25.4% for the control cells and 3.9%, 5.6%, and 4.4% for those exposed to C. albicans, suggesting that co-culture with C. albicans inhibits the apoptosis of OLP keratinocytes. Conclusions: C. albicans activates the TLR2/MyD88/NF-κB signaling pathway in OLP keratinocytes, resulting in increased cytokine expression and decreased keratinocyte apoptosis. Two key events in the pathogenesis of OLP and its progression to malignancy, namely increased inflammation and decreased apoptosis, were induced by exposure to C. albicans. Thus, targeting this signaling pathway may represent a novel therapeutic strategy to prevent OLP malignant transformation.

scholarly journals A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

2019 ◽  
Author(s):  
Hong He ◽  
Mengyun Zhang ◽  
Xinyu Xia ◽  
Haiping Yang ◽  
Qiao Peng
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Random Amplified Polymorphic Dna ◽  
Type I ◽  
Similarity Coefficients ◽  
Professional Recognition ◽  
Normal Individuals ◽  
Polymerase Chain ◽  
Oral Lichen

Abstract Objectives：This study aimed to investigate the prevalence and genotypic profiles of Candida albicans from patients with oral lichen planus (OLP). Materials andMethods：Genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reaction were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results：The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients S AB were 100%, and inter-groups similarity coefficients S AB were less than 30%. Conclusions：The genotypic evidence of C. albicans in OLP might inferred an endogenous infection and some etiologic sense contributing to professional recognition on the development and progression of OLP for more suitable diagnose and treatment.

scholarly journals A pilot study: A possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

2020 ◽  
Author(s):  
Hong He ◽  
Xinyu Xia ◽  
Haiping Yang ◽  
Qiao Peng ◽  
Jiaoer Zheng
Keyword(s):  
Candida Albicans ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Random Amplified Polymorphic Dna ◽  
Type I ◽  
Similarity Coefficients ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Normal Individuals ◽  
Oral Lichen

Abstract Background: The aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP). Materials and Methods: Positive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software. Results: The prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87%, 18.75%, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%. Conclusions: The genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

A scoring system for mucosal disease severity with special reference to oral lichen planus

2007 ◽  
Vol 157 (4) ◽  
pp. 765-770 ◽  
Author(s):  
M. Escudier ◽  
N. Ahmed ◽  
P. Shirlaw ◽  
J. Setterfield ◽  
A. Tappuni ◽  
...  
Keyword(s):  
Special Reference ◽  
Disease Severity ◽  
Lichen Planus ◽  
Scoring System ◽  
Oral Lichen Planus ◽  
Mucosal Disease ◽  
Oral Lichen

scholarly journals Impact of Oral Lichen Planus on Oral Health-Related Quality of Life: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 11 (2) ◽  
pp. 272-286
Author(s):  
Monal Yuwanati ◽  
Shailesh Gondivkar ◽  
Sachin C. Sarode ◽  
Amol Gadbail ◽  
Gargi S. Sarode ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Oral Health ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Health Related Quality ◽  
Related Quality ◽  
Health Related ◽  
The Impact ◽  
Oral Lichen

Oral health-related quality of life (OR-QoL) measurement in patients with oral lichen planus (OLP) can provide valuable information for the optimal management of their clinical conditions. The main objective of the present study was to assess the OR-QoL of patients with OLP as measured by the short-form Oral Health Impact profile-14 (OHIP-14) questionnaire. PubMed/MEDLINE, ISI/Web of Science, clinical trial registry, Embase, Scopus, and grey literature (via Google Scholar and Scilit) were searched. Reviewers independently screened titles/abstracts, assessed full-text articles, extracted data, and appraised their quality. Random effect analysis along with subgroup analysis for age, gender, and clinical type was performed. Seventeen studies were included. Mean overall OH-QoL was 15.20, [95% CI 12.176, 18.231]; a higher OHIP-14 score was seen in OLP patients, resulting in poor OH-QoL. The impact of OLP on OH-QoL life was moderate as compared to healthy subjects. However, medical treatment of the disease improved the OH-QoL and thus reduced the impact of OLP on it. OH-QoL among patients with OLP is generally poor. Clinicians and physicians should consider the OH-QoL of these patients as part of patients’ evaluation and modulate the administered treatment based on the OH-QoL response.

Mucosal disease

2018 ◽  
Author(s):  
Kathy Taghipour
Keyword(s):  
Autoimmune Disease ◽  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Pemphigus Vulgaris ◽  
Stevens Johnson Syndrome ◽  
Mucosal Disease ◽  
Johnson Syndrome ◽  
Dermatological Condition ◽  
A Cell ◽  
Oral Lichen

This chapter discusses the following mucous membrane disorders: pemphigus vulgaris, lichen planus, and Stevens–Johnson syndrome. Pemphigus vulgaris is an autoimmune disease that affects the skin and the mucosal membranes with blisters and erosions. Lichen planus is a cell-mediated immunological mucocutaneous disease; oral lichen planus may present with erosions, white streaks, or plaques in the oral cavity. Stevens–Johnson syndrome is an emergency dermatological condition in which an immunological hypersensitivity causes erosions and inflammation of mucosal membranes and the skin. As well as providing definitions of these diseases, this chapter discusses their etiology, typical symptoms, uncommon symptoms, demographics, natural history, complications, diagnostic approach, other diagnoses that should be considered, prognosis, and treatment.

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