Components of the Alternative Pathway of Complement in Otitis Media with Effusion

1985 ◽  
Vol 93 (5) ◽  
pp. 607-611 ◽  
Author(s):  
Michael J. Parker ◽  
Donald A. Leopold ◽  
Ann E. Stitzel ◽  
Thomas R. Welch ◽  
Leonard B. Weiner ◽  
...  
Keyword(s):  
Otitis Media ◽  
Clinical Course ◽  
Otitis Media With Effusion ◽  
Past History ◽  
Alternative Pathway ◽  
Factor H ◽  
Complement Components ◽  
Bacterial Cultures ◽  
Middle Ear Fluid ◽  
Alternative Pathway Of Complement

An evaluation of the alternative pathway of complement was undertaken in patients with otitis media with effusion (OME). Middle ear fluid (MEF) and serum specimens were obtained from 34 patients at the time of elective myringotomy for OME. Bacterial, viral, and mycoplasma cultures were made for all specimens of the fluids. Immunochemical determinations by radial immunodiffusion were performed for C3, C5, factor B, properdin, factor H, factor I, and albumin. Each patient's recent clinical course and past history were reviewed. The results of all viral and mycoplasma cultures were negative. Three of 55 bacterial cultures were positive for type B Haemophilus Influenzae. All components of the alternative pathway measured were found to be present in varying amounts in MEF. When the levels of the complement components were compared to the clinical factors studied, there were no observable differences. These data suggest that components of the alternative pathway Of complement are present in OME and are not useful in predicting the clinical course or outcome of this disorder.

scholarly journals MICROORGANISMS IN CHRONIC OTITIS MEDIA WITH EFFUSION

2003 ◽  
Vol 41 (142) ◽  
pp. 314-317
Author(s):  
Anil Kumar Jha ◽  
J B Singh ◽  
S P Raut
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Negative Pressure ◽  
Otitis Media With Effusion ◽  
Chronic Otitis Media ◽  
Bacterial Cultures ◽  
Middle Ear Fluid ◽  
Nature Ofthe

A total of 100 patient with otitis media effusion obtained from patients suffering fromchronic otitis media with effusions was examined for bacterial smear and culture. Inmucoid effusion 82% showed positive bacterial smear, only 35% yielded positivebacterial culture. Bacterial cultures rate was higher in serous (50%) effusion. Theisolation of common pathogens accounted for the remaining 42%. The high incidenceof microorganisms in the middle ear effusions in the present study indicates bacterialcontribution in many cases of otitis media effusion. Concerning the sterile nature ofthe middle ear fluid some investigators suggested that the effusions are transudatesand are created by a negative pressure in the tympanum due to a malfunctioningEustachian tube.2It was suggested that failure to isolate organisms may be partly dueto the antimicrobial characteristics of effusions. The purpose of this study is to showpossible role of bacteria in Middle Ear Effusions.Key Words: Otitis Media, Effusion, Microorganisms.

scholarly journals Activation of the alternative pathway of complement by monoclonal lambda light chains in membranoproliferative glomerulonephritis.

1992 ◽  
Vol 175 (4) ◽  
pp. 939-950 ◽  
Author(s):  
S Meri ◽  
V Koistinen ◽  
A Miettinen ◽  
T Törnroth ◽  
I J Seppälä
Keyword(s):  
Membranoproliferative Glomerulonephritis ◽  
Alternative Pathway ◽  
Immunoglobulin A ◽  
Fluid Phase ◽  
Normal Serum ◽  
Factor H ◽  
Control Factor ◽  
Dependent Manner ◽  
Complement Components ◽  
Alternative Pathway Of Complement

Immunopathological evidence suggests that activation of the alternative pathway of complement (AP) is involved in membranoproliferative glomerulonephritis (MPGN) and in immunoglobulin A nephropathy. In this report we describe an AP dysfunction-associated factor that was isolated from the serum and urine of a patient with hypocomplementemic MPGN. Extensive glomerular deposits of C3, properdin, and of the terminal complement components were observed in the kidney of the patient. In her serum the AP hemolytic activity was virtually absent. When mixed with fresh normal serum, the patient's serum induced a 96% C3 conversion during a 30-min incubation at +37 degrees C. This activity was found to be due to a circulating factor that by immunochemical characterization proved to be a 46-kD monoclonal immunoglobulin lambda light (L) chain dimer (lambda L). Purified lambda L, but not control lambda or kappa L chains from patients with L chain disease, activated the AP in a dose- and ionic strength-dependent manner. Functionally, lambda L was differentiated from C3 nephritic factor (an autoantibody against the AP C3 convertase, C3bBb) by its inability to bind to and stabilize the C3bBb enzyme. Instead, lambda L was observed to interact directly with the AP control factor H. Thus, lambda L represents a novel type of immunoglobulin-related AP-activating factor with the capacity to initiate alternative complement pathway activation in the fluid phase.

Prognostic factors in otitis media with effusion

1988 ◽  
Vol 102 (11) ◽  
pp. 989-991 ◽  
Author(s):  
P. M. Robinson
Keyword(s):  
Prognostic Factors ◽  
Otitis Media ◽  
Otitis Media With Effusion ◽  
Past History ◽  
Waiting List ◽  
Repeat Surgery ◽  
History Of

AbstractTo determine which factors were associated recurrence of ottitis media with effusion following grommet insertion, the casenotes of 323 who had a total of 485 operations for grommet insertion were reviewed. The incidence of repeat surgery was higher in those aged between four and six years, in those having grommet insertion between the months of July and October and in those cases in which the grommet was extruded within six months. Recurrence was not related to sex of patient, month of listing for surgery, adenoidectomy, tonsillectomy, length of time on the waiting list or past history of previous grommet insertion.

Penetration of Ceftazidime Into Middle Ear Fluid in Children With Otitis Media With Effusion

1998 ◽  
Vol 108 (5) ◽  
pp. 662-664
Author(s):  
Pierre Bégué ◽  
Erea-Noël Garabédian ◽  
Catherine Bertrand ◽  
Bernard Aubert ◽  
Dan Chiche
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Otitis Media With Effusion ◽  
Middle Ear Fluid

Fibrinolytic Activity of Middle Ear Fluid in Otitis Media with Effusion

1985 ◽  
Vol 12 ◽  
pp. S138-S140 ◽  
Author(s):  
Katsuichiro Ohsaki ◽  
Mamoru Kimura ◽  
Ikuo Inokuchi ◽  
Tomoaki Sugiura, ◽  
Seikyo So ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Fibrinolytic Activity ◽  
Otitis Media With Effusion ◽  
Middle Ear Fluid

scholarly journals Prevention of Fatal C3 Glomerulopathy by Recombinant Complement Receptor of the Ig Superfamily

2018 ◽  
Vol 29 (8) ◽  
pp. 2053-2059 ◽  
Author(s):  
Xiaoxu Wang ◽  
Menno Van Lookeren Campagne ◽  
Kenneth J. Katschke ◽  
Damodar Gullipalli ◽  
Takashi Miwa ◽  
...  
Keyword(s):  
Alternative Pathway ◽  
Factor H ◽  
Complement Receptor ◽  
C3 Glomerulopathy ◽  
Fibrin Deposition ◽  
Medical Need ◽  
Potential Efficacy ◽  
Ig Superfamily ◽  
Life Threatening ◽  
Alternative Pathway Of Complement

Background C3 glomerulopathy (C3G) is a life-threatening kidney disease caused by dysregulation of the alternative pathway of complement (AP) activation. No approved specific therapy is available for C3G, although an anti-C5 mAb has been used off-label in some patients with C3G, with mixed results. Thus, there is an unmet medical need to develop other inhibitors of complement for C3G.Methods We used a murine model of lethal C3G to test the potential efficacy of an Fc fusion protein of complement receptor of the Ig superfamily (CRIg-Fc) in the treatment of C3G. CRIg-Fc binds C3b and inhibits C3 and C5 convertases of the AP. Mice with mutations in the factor H and properdin genes (FHm/mP−/−) develop early-onset C3G, with AP consumption, high proteinuria, and lethal crescentic GN.Results Treatment of FHm/mP−/− mice with CRIg-Fc, but not a control IgG, inhibited AP activation and diminished the consumption of plasma C3, factor B, and C5. CRIg-Fc–treated FHm/mP−/− mice also had significantly improved survival and reduced proteinuria, hematuria, BUN, glomerular C3 fragment, C9 and fibrin deposition, and GN pathology scores.Conclusions Therapeutics developed on the basis of the mechanism of action of soluble CRIg may be effective for the treatment of C3G and should be explored clinically.

Complement alternative pathway activation associated with pulmonary hypertension in lupus nephritis patients

Lupus ◽  
2019 ◽  
Vol 28 (9) ◽  
pp. 1051-1061
Author(s):  
Q Li ◽  
H Li ◽  
J Shi ◽  
B He ◽  
F Yu
Keyword(s):  
Pulmonary Hypertension ◽  
Lupus Nephritis ◽  
Lupus Erythematosus ◽  
Alternative Pathway ◽  
Factor H ◽  
Renal Outcome ◽  
Complement Alternative Pathway ◽  
Complement Components ◽  
Pathway Activation ◽  
Excessive Activation

Pulmonary hypertension occurs in systemic lupus erythematosus (SLE) for several reasons, such as vasculopathy. Previous studies have indicated that the excessive activation of the complement alternative pathway might be involved in the pathogenesis of lupus nephritis, especially in the absence of factor H or its functional impairment. However, the clinical and pathological significance of the alternative complement activation in lupus nephritis patients with pulmonary hypertension remains elusive. The data on patients with pulmonary hypertension and non-pulmonary hypertension lupus nephritis were retrospectively analyzed in our centre. Major plasma levels of complement components were evaluated. The depositions of Bb, C3d and C5b-9 in the lung specimens of pulmonary hypertension combined with SLE patients were detected by immunofluorescence staining. Among 352 lupus nephritis cases, 24 were diagnosed with pulmonary hypertension and 328 with non-pulmonary hypertension. Higher levels of Bb and lower levels of factor H were detected in the pulmonary hypertension group in comparison with the negative group ( P = 0.049, P = 0.024, respectively). Pulmonary hypertension was a risk factor for renal outcome as deduced by the log-rank and Cox test for survival analysis. C3d, C5b-9 and Bb were found to be positive in lung specimens of lupus nephritis patients with pulmonary hypertension. We concluded that activation of the complement alternative pathway may be involved in the pathogenesis of pulmonary hypertension in lupus nephritis.

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