Culture Techniques and Potential Research Applications of Human Hair Follicle Cells In Vitro

1985 ◽  
Vol 13 (1) ◽  
pp. 8-37
Author(s):  
A.J.M. Vermorken

Since 1980, when human hair follicle cells were cultured in vitro for the first time, a whole series of techniques have been developed that render hair follicle keratinocytes as easy to handle in culture as fibroblasts. As a consequence, one can conclude that the need for a method providing for the routine cultivation of easily obtainable human primary epithelial cells has now been met, and it may be expected that more and more workers will use hair follicle keratinocytes for studies that specifically require human epithelial cells. The ease of culture and the ready availability of material may encourage workers to consider human hair follicle cell culture before either animal models or cultures of cells derived from invasive skin biopsies.

1990 ◽  
Vol 1 (2) ◽  
pp. 131
Author(s):  
Y. Sadamoto ◽  
S. Arase ◽  
R. Kuwana ◽  
K. Takeda

1990 ◽  
Vol 52 (1) ◽  
pp. 3-7
Author(s):  
Ryuichiro KUWANA ◽  
Seiji ARASE ◽  
Yasushi SADAMOTO ◽  
Kimitaka KANNO ◽  
Katsuyuki TAKEDA

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Lei Xiao ◽  
Xia Zhang ◽  
Zhiyi Chen ◽  
Yuting Li ◽  
Bing Li ◽  
...  

Extractions from Phaeodactylum tricornutum have been widely studied and evaluated to various biological effects. The aim of this study was to investigate the promotional effect of P. tricornutum extract (PTE) on the ERK1/2 signaling pathway involved in hair follicle cell proliferation. In order to illuminate the enhancement of PTE on hair growth by promoting proliferation of hair follicle cells, the activities of human hair follicle outer root sheath cell (HFORSC), human hair follicle germinal matrix cells (HFGMC), and hair epithelial melanocytes (HEM) were observed under PET treatment. Levels of keratins, PKCζ, ERK1/2, and p38 MAPK in hair follicle cells were determined by Western blotting to illustrate the mechanisms of PTE effects on hair growth. Analyzed by GC-MS, the main polyunsaturated fatty acids which were 9.43% of total fatty acids in PTE were linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid. Melanin content and tyrosinase activity in HEM were measured. The results showed that PTE exhibited remarkable enhancement on cell proliferation. Melanin production was inhibited by PTE treatment, while keratin-14, keratin-15, and keratin-17 levels on hair follicle cells were elevated at different concentrations. The promotions of ERK1/2 and p38 MAPK levels indicated that the ERK1/2 signaling pathway is involved in the proliferation of hair follicle cells. These results are the evidence that PTE potentially deserves further study as a new natural candidate for hair care applications.


1988 ◽  
Vol 50 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Ryuichiro KUWANA ◽  
Seiji ARASE ◽  
Yasushi SADAMOTO ◽  
Hideki NAKANISHI ◽  
Katsuyuki TAKEDA

1989 ◽  
Vol 51 (1) ◽  
pp. 66-70 ◽  
Author(s):  
Ryuichiro KUWANA ◽  
Seiji ARASE ◽  
Yasushi SADAMOTO ◽  
Kimitaka KANNO ◽  
Hideki NAKANISHI ◽  
...  

2015 ◽  
Vol 05 (999) ◽  
pp. 1-1
Author(s):  
Abu Bakar Mohd Hilmi ◽  
Mohd Noor Norhayati ◽  
Ahmad Sukari Halim ◽  
Chin Keong Lim ◽  
Zulkifli Mustafa ◽  
...  

1987 ◽  
Vol 17 (5) ◽  
pp. 779-786 ◽  
Author(s):  
Eckhart Imcke ◽  
Auguste Mayer-da-Silva ◽  
Michael Detmar ◽  
Hartmut Tiel ◽  
Rudolf Stadler ◽  
...  

2020 ◽  
Vol 140 (4) ◽  
pp. 764-773.e4 ◽  
Author(s):  
Rie Takahashi ◽  
Adrienne Grzenda ◽  
Thomas F. Allison ◽  
Jeffrey Rawnsley ◽  
Samuel J. Balin ◽  
...  

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