Primary Effusion Lymphoma Cells Undergoing Human Herpesvirus Type 8 Productive Infection Produce C-Type Retroviral Particles

2008 ◽  
Vol 21 (4) ◽  
pp. 999-1006 ◽  
Author(s):  
A. Tinari ◽  
F. Superti ◽  
M.G. Ammendolia ◽  
C. Chiozzini ◽  
C. Hohenadl ◽  
...  
Keyword(s):  
Particle Production ◽  
Phorbol Esters ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Endogenous Retroviruses ◽  
Productive Infection ◽  
Late Gene Expression ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type ◽  
Type Particle

Primary effusion lymphomas (PELs) are invariably infected by the human herpesvirus 8 (HHV8) that is present in most PEL cells as latent virus but replicates in a subset of permissive cells to produce infectious progeny. Here we show that productively infected PEL cells release C-type retrovirus-like particles encoding an Mn++-dependent RT activity, which is typical of endogenous retroviruses. Strikingly, C-type particles are produced only in cells showing advanced HHV8 morphogenesis. Phorbol esters, which induce productive HHV8 replication and morphogenesis in PEL cells, increase RLP production. Phosphonoacetic acid, a blocker of HHV8 late gene expression, inhibits the production of C-type particles, whereas neutralizing anti-αIFN antibodies, which are known to increase HHV8 assembly, increases C-type particle production. These data suggest that factors expressed in advanced stages of HHV8 reactivation support endogenous C-type particle morphogenesis in PEL cells.

Primary Effusion Lymphoma Cell Lines Harbouring Human Herpesvirus Type-8

2000 ◽  
Vol 36 (5-6) ◽  
pp. 447-456 ◽  
Author(s):  
Antonino Carbone ◽  
Anna M. Cilia ◽  
Annunziata Gloghini ◽  
Daniela Capello ◽  
Tiziana Perin ◽  
...  
Keyword(s):  
Cell Lines ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
Lymphoma Cell ◽  
Herpesvirus Type ◽  
Primary Effusion Lymphoma Cell ◽  
Human Herpesvirus Type

scholarly journals Human herpesvirus type 2 infection of primary murine astrocytes causes disruption of the mitochondrial network and remodeling of the actin cytoskeleton: an in vitro morphological study

2021 ◽  
Author(s):  
Anna Słońska ◽  
Joanna Cymerys ◽  
Marcin Chodkowski ◽  
Piotr Bąska ◽  
Małgorzata Krzyżowska ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Human Herpesvirus ◽  
Productive Infection ◽  
Mitochondrial Morphology ◽  
Mitochondrial Network ◽  
Herpesvirus Type ◽  
Viral Spread ◽  
Human Herpesvirus Type

AbstractHerpesviruses are capable of infecting not only neurons, where they establish latent infection, but also astrocytes. Since astrocytes are important for the functioning of the central nervous system (CNS), their infection may lead to serious neurological disorders. Thus, in the present study we investigated the ability of human herpesvirus type 2 (HHV-2) to infect primary murine astrocytes in vitro and the effect of infection on their mitochondrial network and actin cytoskeleton. In immunofluorescence assays, antibodies against HHV-2 antigens and glial fibrillary acidic protein (GFAP) were used to confirm that the infected cells are indeed astrocytes. Real-time PCR analysis showed a high level of HHV-2 replication in astrocytes, particularly at 168 h postinfection, confirming that a productive infection had occurred. Analysis of mitochondrial morphology showed that, starting from the first stage of infection, HHV-2 caused fragmentation of the mitochondrial network and formation of punctate and tubular structures that colocalized with virus particles. Furthermore, during the late stages of infection, the infection affected the actin cytoskeleton and induced formation of actin-based cellular projections, which were probably associated with enhanced intracellular spread of the virus. These results suggest that the observed changes in the mitochondrial network and actin cytoskeleton in productively infected astrocytes are required for effective replication and viral spread in a primary culture of astrocytes. Moreover, we speculate that, in response to injury such as HHV-2 infection, murine astrocytes cultured in vitro undergo transformation, defined in vivo as reactive astrocytosis.

scholarly journals A Rare Case of Extracavitary Primary Effusion Lymphoma in the Bladder and Ureter

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Jiankun Tong ◽  
Sana Jadallah ◽  
William H. Rodgers ◽  
Gabriel Jung ◽  
Malvina Fulman ◽  
...  
Keyword(s):  
Rare Case ◽  
Primary Effusion Lymphoma ◽  
Human Herpesvirus ◽  
B Cell Lymphoma ◽  
The Body ◽  
Herpesvirus Type ◽  
First Case ◽  
Immunodeficiency Virus ◽  
Human Herpesvirus Type ◽  
Chinese Male

Primary effusion lymphoma (PEL) is a rare and very aggressive large B-cell lymphoma usually presenting as serous effusions without a tumor mass. It is universally associated with human herpesvirus type-8 (HHV-8) infection. It most commonly occurs in the body cavities and rarely develops as solid tumor masses in the wall of cavity and other organs, and it has been termed as extracavitary PEL. Extracavitary PEL has been reported in the lymph nodes and extranodal sites. Here we report a rare case of extracavitary PEL occurring in the bladder and ureter of a human immunodeficiency virus (HIV)-negative 76-year-old Chinese male, presenting with right leg swelling, erythema, and pain. To the best of our knowledge, this is the first case of extracavitary PEL presenting in the bladder and ureter.

Increased prevalence of human herpesvirus type 8 (HHV-8) genome among blood donors from North-Western Argentina

2016 ◽  
Vol 89 (3) ◽  
pp. 518-527 ◽  
Author(s):  
María Laura Hulaniuk ◽  
Oscar Torres ◽  
Sonia Bartoli ◽  
Lisandro Fortuny ◽  
Leandro Burgos Pratx ◽  
...  
Keyword(s):  
Blood Donors ◽  
Human Herpesvirus ◽  
Herpesvirus Type ◽  
Human Herpesvirus Type ◽  
North Western
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