MCP-1 produced by keratinocytes is associated with leucocyte recruitment during elicitation of nickel-induced occupational allergic contact dermatitis

2017 ◽  
Vol 34 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Weihua Mai ◽  
Dongqing Lu ◽  
Xingwei Liu ◽  
Ling Chen
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Messenger Rna ◽  
Late Phase ◽  
Nickel Sulfate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Leucocyte Migration ◽  
Nickel Exposure ◽  
Temporal Expression Pattern ◽  
Allergic Contact

To investigate the expression profile of monocyte chemoattractant peptide-1 (MCP-1) by keratinocytes after nickel exposure and to identify its role for leucocyte migration during nickel-induced occupational allergic contact dermatitis (OACD), 26 workers diagnosed with nickel-induced OACD were enrolled. Skin biopsies from the positive nickel-challenged sites at different time points were assessed by immunohistochemistry (IHC) for MCP-1, CD68, CD45RO, and in situ hybridization (ISH) for MCP-1, using chronic periumbilical dermititis as controls. The expressions of MCP-1 in HaCaT cell culture after nickel treatment were quantified by enzyme-linked immunosorbent assay. The results showed that at positive nickel-challenged sites, strong expressions of MCP-1, both messenger RNA (mRNA) and protein, were detected in the basal keratinocytes during the early phase (24–48 h after nickel application), paralleled by the recruitment of CD68+ and CD45RO+ cells to the skin compartments. The expressions of MCP-1 declined gradually in the late phase (72–96 h after nickel application). Treatment with nickel sulfate at noncytotoxic concentrations (0.01–100 µM) induced a concentration-related elevation of MCP-1 expression by HaCaT cells compared to the untreated cells. The data indicated that a temporal expression pattern of MCP-1 produced by keratinocytes after nickel exposure was involved in the complex process of mononuclear cell infiltration during elicitation of nickel-induced OACD. Targeting MCP-1 might be a potential therapeutic strategy for OACD.

scholarly journals Survey of Occupational Allergic Contact Dermatitis and Patch Test among Clothing Employees in Beijing

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Yu-Xin Chen ◽  
Bing-Ai Gao ◽  
Hai-Yan Cheng ◽  
Lin-Feng Li
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Nickel Sulfate ◽  
Clothing Industry ◽  
Formaldehyde Resin ◽  
Cross Sectional ◽  
Hand Dermatitis ◽  
The Face ◽  
Allergic Contact ◽  
Occupational Contact

Occupational population-based epidemiological data relating to occupational contact allergies in the Chinese clothing industry are limited. To investigate the prevalence of occupational allergic contact dermatitis (OACD) and to identify the causative allergens among clothing employees in China, a cross-sectional study was conducted in 529 clothing employees at 12 clothing factories in Beijing. All employees were subjected to an interview using self-administered questionnaire and skin examination, and those who were diagnosed with occupational contact dermatitis (OCD) were patch tested. In the present survey, we found that the overall 1-year prevalence of OACD among the clothing employees was 8.5%. The 1-year prevalence of OACD among workers (10.8%) was significantly higher than that among managers (3.2%). The lesions were primarily on the hands and wrists in workers, but the face and neck in managers. The major allergens were nickel sulfate and cobalt dichloride in workers and colophony andp-tert-butylphenol formaldehyde resin in managers. In conclusion, workers are at a higher risk of OACD compared with managers in the Chinese clothing industry. In addition to hand dermatitis in workers, airborne contact dermatitis on the face and neck should be also addressed in managers.

scholarly journals Main Allergens Observed in Patients with Contact Dermatitis in a Brazilian Population Group

2015 ◽  
Vol 9 (1) ◽  
pp. 21-24
Author(s):  
Zamir Calamita ◽  
Ana Cristina Rizzo Alonso ◽  
Lorena Carla Oliveira da Costa ◽  
Andrea Bronhara Pelá Calamita
Keyword(s):  
Retrospective Study ◽  
Contact Dermatitis ◽  
Medical School ◽  
Allergic Contact Dermatitis ◽  
Nickel Sulfate ◽  
Population Group ◽  
Good Strategy ◽  
Patch Tests ◽  
Skin Contact ◽  
Allergic Contact

Background: The skin contact test or patch test is considered to be a fundamental tool for investigating allergic contact dermatitis (ACD). Better knowledge on the prevalence of allergens in the environment is a good strategy for enabling a better approach towards contact dermatitis (CD) cases. Objective: The objective of the present study was to evaluate the prevalence of the main allergens of ACD in a population group in the interior of the state of São Paulo, Brazil. Methods: The results from 368 patch tests on adult patients with CD were evaluated through a retrospective study under the supervision of the Discipline of Allergy and Clinical Immunopathology and the Discipline of Dermatology at the Marília Medical School. Results: Mean age was 41.2 (± 17.2) years, with predomination of women (71.5%). The majority of the patients (91.3%) presented reactivity to at least one substance. Nickel sulfate and the perfume mix stood out as the most allergenic substances. Conclusion: Among the variety of substances to be tested in an etiological investigation for CD, perfumes and nickel are especially likely to be allergens in this population group.

Comparative Study of IQ-Ultra and Finn Chambers Test Methodologies in Detecting 10 Common Standard Allergens that Cause Allergic Contact Dermatitis

2012 ◽  
Vol 16 (1) ◽  
pp. 18-22 ◽  
Author(s):  
Joseph Doumit ◽  
Melanie Pratt
Keyword(s):  
Contact Dermatitis ◽  
Allergic Contact Dermatitis ◽  
Patch Testing ◽  
Potassium Dichromate ◽  
Nickel Sulfate ◽  
Formaldehyde Resin ◽  
Perfect Agreement ◽  
Patch Tests ◽  
The North ◽  
Allergic Contact

Background: Patch testing is routinely used in contact dermatitis clinics because it is the gold standard for the evaluation of potential allergic contact dermatitis. Objective: The study was undertaken to evaluate possible differences in reactivity between the Finn Chamber and IQ-Ultra patch-testing methodologies. Methods: Patients were patch-tested simultaneously with the Finn Chamber and IQ-Ultra patch tests. Ten standard allergens set by the North American Contact Dermatitis Group were used for both techniques. Results: Both patch tests had a significant agreement in detecting all of the allergens. An “almost perfect agreement” was noted for ethylenediamine dihydrochloride, quaternium-15, mercapto mix, black rubber mix, balsam of Peru, and nickel sulfate; “substantial agreement” for formaldehyde, bisphenol A epoxy resin, and 4-tert-butylphenol formaldehyde resin; and “moderate agreement” for potassium dichromate. Conclusion: The Finn Chamber and IQ-Ultra patch tests had a good agreement in the detection of the 10 standard allergens that were tested.

Sign in / Sign up

Export Citation Format

Share Document