Chitosan Beads as Templates for Layer-by-Layer Assembly and their Application in the Sustained Release of Bioactive Agents

Osseointegration of dental, craniofacial, and orthopedic implants is critical for their long-term success. Multifunctional surface treatment of implants was found to significantly improve cell adhesion and induce osteogenic differentiation of dental-derived stem cells in vitro. Moreover, local and sustained release of antibiotics via nanolayers from the surface of implants can present unparalleled therapeutic benefits in implant dentistry. Here, we present a layer-by-layer surface treatment of titanium implants capable of incorporating BMP-2–mimicking short peptides and gentamicin to improve their osseointegration and antibacterial features. Additionally, instead of conventional surface treatments, we employed polydopamine coating before layer-by-layer assembly to initiate the formation of the nanolayers on rough titanium surfaces. Cytocompatibility analysis demonstrated that modifying the titanium implant surface with layer-by-layer assembly did not have adverse effects on cellular viability. The implemented nanoscale coating provided sustained release of osteoinductive peptides with an antibacterial drug. The surface-functionalized implants showed successful osteogenic differentiation of periodontal ligament stem cells and antimicrobial activity in vitro and increased osseointegration in a rodent animal model 4 wk postsurgery as compared with untreated implants. Altogether, our in vitro and in vivo studies suggest that this approach can be extended to other dental and orthopedic implants since this surface functionalization showed improved osseointegration and an enhanced success rate.

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Graphene Oxide-Assisted Accumulation and Layer-by-Layer Assembly of Antibacterial Peptide for Sustained Release Applications

ACS Applied Materials & Interfaces ◽

10.1021/acsami.8b07417 ◽

2018 ◽

Vol 10 (29) ◽

pp. 24937-24946 ◽

Cited By ~ 13

Author(s):

Meiwen Cao ◽

Wenjing Zhao ◽

Lei Wang ◽

Ruiheng Li ◽

Haoning Gong ◽

...

Keyword(s):

Graphene Oxide ◽

Sustained Release ◽

Antibacterial Peptide ◽

Layer By Layer ◽

Layer By Layer Assembly ◽

Layer Assembly

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Pulmonary sustained release of insulin from microparticles composed of polyelectrolyte layer-by-layer assembly

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2014.02.006 ◽

2014 ◽

Vol 466 (1-2) ◽

pp. 96-108 ◽

Cited By ~ 9

Author(s):

Kiran Prakash Amancha ◽

Shantanu Balkundi ◽

Yuri Lvov ◽

Alamdar Hussain

Keyword(s):

Sustained Release ◽

Layer By Layer ◽

Polyelectrolyte Layer ◽

Layer By Layer Assembly ◽

Layer Assembly

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Complex formation of modified chitosan and carboxymethyl cellulose and its effect on paper properties

TAPPI Journal ◽

10.32964/tj8.6.29 ◽

2009 ◽

Vol 8 (6) ◽

pp. 29-35 ◽

Cited By ~ 11

Author(s):

PEDRAM FATEHI ◽

LIYING QIAN ◽

RATTANA KITITERAKUN ◽

THIRASAK RIRKSOMBOON ◽

HUINING XIAO

Keyword(s):

Carboxymethyl Cellulose ◽

Polymer System ◽

Layer By Layer ◽

Paper Strength ◽

Anionic Polymer ◽

Paper Properties ◽

Modified Chitosan ◽

Layer By Layer Assembly ◽

Polyelectrolyte Titration ◽

Layer Assembly

The application of an oppositely charged dual polymer system is a promising approach to enhance paper strength. In this work, modified chitosan (MCN), a cationic polymer, and carboxymethyl cellulose (CMC), an anionic polymer, were used sequentially to improve paper strength. The adsorption of MCN on cellulose fibers was analyzed via polyelectrolyte titration. The formation of MCN/CMC complex in water and the deposition of this complex on silicon wafers were investigated by means of atomic force microscope and quasi-elastic light scattering techniques. The results showed that paper strength was enhanced slightly with a layer-by-layer assembly of the polymers. However, if the washing stage, which was required for layer-by-layer assembly, was eliminated, the MCN/CMC complex was deposited on fibers more efficiently, and the paper strength was improved more significantly. The significant improvement was attributed to the extra development of fiber bonding, confirmed further by scanning electron microscope observation of the bonding area of fibers treated with or without washing. However, the brightness of papers was somewhat decreased by the deposition of the complex on fibers. Higher paper strength also was achieved using rapid drying rather than air drying.

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