Cartilage tissue engineering with controllable shape using a poly(lactic-co-glycolic acid)/collagen hybrid scaffold

2013 ◽  
Vol 28 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Wenda Dai ◽  
Zhenjun Yao ◽  
Jian Dong ◽  
Naoki Kawazoe ◽  
Chi Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Glycolic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold

The potential of 3-dimensional construct engineered from poly(lactic-co-glycolic acid)/fibrin hybrid scaffold seeded with bone marrow mesenchymal stem cells for in vitro cartilage tissue engineering

2015 ◽  
Vol 47 (4) ◽  
pp. 420-430 ◽  
Author(s):  
Rozlin Abdul Rahman ◽  
Norhamiza Mohamad Sukri ◽  
Noorhidayah Md Nazir ◽  
Muhammad Aa’zamuddin Ahmad Radzi ◽  
Ahmad Hafiz Zulkifly ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Glycolic Acid ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold ◽  
3 Dimensional ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Biodegradable CSMA/PECA/Graphene Porous Hybrid Scaffold for Cartilage Tissue Engineering

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
JinFeng Liao ◽  
Ying Qu ◽  
BingYang Chu ◽  
XiaoNing Zhang ◽  
ZhiYong Qian
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold

The Development of Gelatin/Chitosan Hybrid Scaffold for Mouse Embryonic Stem Cell Line ATDC5

2010 ◽  
Vol 123-125 ◽  
pp. 395-398
Author(s):  
Yuan Li ◽  
Naoki Matsuda ◽  
Xu Xu Bao ◽  
Akira Teramoto ◽  
Koji Abe
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Cell Line ◽  
Cartilage Tissue Engineering ◽  
Embryonic Stem Cell Line ◽  
Cartilage Tissue ◽  
Coating Film ◽  
Stem Cell Line ◽  
Tissue Culture Dish ◽  
Hybrid Scaffold

Functional scaffolds fabricated from biopolymers are of great importance for tissue engineering. In this paper, by mimicking the combination of two major components in Extracellular Matrix – collagen and polysaccharide respectively, we fabricated gelatin and chitosan hybrid sponge for cartilage tissue engineering. Extending previous results of gelatin/chitosan film showed that all the hybrid coating film (chitosan: gelatin= 4:1; 1:1; 1:4) can support the adhesion of ATDC5 that comparable to Tissue Culture Dish (TCD). More importantly, stem cell line ATDC5 on coating dish of chitosan: gelatin=1:4 showed enhanced differentiation rate than that on other films and TCD. For three dimensional scaffolds, we fabricated hybrid sponge scaffold of chitosan: gelatin=4:1, 2:1, 1:1, 1:2, 1:4 respectively. Morphological characterizations of the sponges showed that this kind of gelatin/chitosan hybrid scaffold is promising for application in cartilage tissue engineering.

scholarly journals Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering

2021 ◽  
Vol 22 (7) ◽  
pp. 3635
Author(s):  
Jan-Tobias Weitkamp ◽  
Michael Wöltje ◽  
Bastian Nußpickel ◽  
Felix N. Schmidt ◽  
Dilbar Aibibu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Marker Gene ◽  
Cartilage Tissue Engineering ◽  
Biomechanical Properties ◽  
Cartilage Tissue ◽  
Hybrid Scaffold ◽  
Matrix Deposition ◽  
Chondrogenic Marker ◽  
Tgf Β1

A continuing challenge in cartilage tissue engineering for cartilage regeneration is the creation of a suitable synthetic microenvironment for chondrocytes and tissue regeneration. The aim of this study was to develop a highly tunable hybrid scaffold based on a silk fibroin matrix (SM) and a hyaluronic acid (HA) hydrogel. Human articular chondrocytes were embedded in a porous 3-dimensional SM, before infiltration with tyramine modified HA hydrogel. Scaffolds were cultured in chondropermissive medium with and without TGF-β1. Cell viability and cell distribution were assessed using CellTiter-Blue assay and Live/Dead staining. Chondrogenic marker expression was detected using qPCR. Biosynthesis of matrix compounds was analyzed by dimethylmethylene blue assay and immuno-histology. Differences in biomaterial stiffness and stress relaxation were characterized using a one-step unconfined compression test. Cell morphology was investigated by scanning electron microscopy. Hybrid scaffold revealed superior chondro-inductive and biomechanical properties compared to sole SM. The presence of HA and TGF-β1 increased chondrogenic marker gene expression and matrix deposition. Hybrid scaffolds offer cytocompatible and highly tunable properties as cell-carrier systems, as well as favorable biomechanical properties.

A cell leakproof PLGA-collagen hybrid scaffold for cartilage tissue engineering

2009 ◽  
Vol 26 (3) ◽  
pp. 819-826 ◽  
Author(s):  
Naoki Kawazoe ◽  
Chieko Inoue ◽  
Tetsuya Tateishi ◽  
Guoping Chen
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Hybrid Scaffold ◽  
A Cell
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