Selective Activity of Various Nucleoside and Nucleotide Analogues against Human Herpesvirus 6 and 7

1997 ◽  
Vol 8 (1) ◽  
pp. 24-31 ◽  
Author(s):  
K Takahashi ◽  
M Suzuki ◽  
Y Iwata ◽  
S Shigeta ◽  
K Yamanishi ◽  
...  
Keyword(s):  
Large Scale ◽  
Human Herpesvirus ◽  
Tetrazolium Salt ◽  
Human Herpesvirus 6 ◽  
Phosphonoformic Acid ◽  
Highly Sensitive ◽  
Acyclic Nucleoside ◽  
Herpesvirus 6 ◽  
Acyclic Nucleoside Phosphonate ◽  
Large Scale Screening

We have developed a new sensitive enzyme immunoassay (EIA) and MTT (tetrazolium salt) assay for screening compounds against two variants of human herpesvirus 6 (HHV-6A, HHV-6B) and human herpesvirus 7 (HHV-7) and evaluated the anti-HHV-6 and HHV-7 activity of a series of anti-herpesvirus compounds and acyclic nucleoside phosphonate analogues. The results indicate that the pattern of activity of these compounds against these betaherpesviruses is similar to that for human cytomegalovirus (HCMV). The highest potency and selectivity against the two variants of HHV-6 and HHV-7 was demonstrated by S2242 (N7-isomer of 6-deoxy-ganciclovir). Also, ganciclovir (GCV), foscarnet, (phosphonoformic acid; PFA) and the acyclic nucleoside phosphonate analogues such as cidofovir (HPMPC) exhibited selective inhibitory activity against these viruses. Thymidine kinase (TK)-dependent drugs (acyclovir, ACV; brivudin, BVDU; and sorivudine, BVaraU) showed little, if any, activity. These results suggest a structural homology of the DNA polymerase and a lack of TK gene among these three betaherpesviruses (HHV-6, HHV-7 and HCMV). The finding that HHV-7 was highly sensitive to GCV also suggests that HHV-7 may have an HCMV-UL97-homologue gene for the phosphorylation of GCV. The present EIA method is more rapid and sensitive than the previously reported procedures and could be useful for the large-scale screening of compounds against HHV-6 and HHV-7.

Antiviral activity of selected acyclic nucleoside analogues against human herpesvirus 6

1995 ◽  
Vol 28 (4) ◽  
pp. 343-357 ◽  
Author(s):  
D. Reymen ◽  
L. Naesens ◽  
J. Balzarini ◽  
A. Holý ◽  
H. Dvořáková ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Human Herpesvirus ◽  
Nucleoside Analogues ◽  
Human Herpesvirus 6 ◽  
Acyclic Nucleoside Analogues ◽  
Acyclic Nucleoside ◽  
Herpesvirus 6

Clinical Features of Infants With Primary Human Herpesvirus 6 Infection (Exanthem Subitum, Roseola Infantum)

PEDIATRICS ◽  
1994 ◽  
Vol 93 (1) ◽  
pp. 104-108
Author(s):  
Yoshizo Asano ◽  
Tetsushi Yoshikawa ◽  
Sadao Suga ◽  
Ikuko Kobayashi ◽  
Toshihiko Nakashima ◽  
...  
Keyword(s):  
Clinical Features ◽  
Large Scale ◽  
Clinical Signs ◽  
Clinical Manifestations ◽  
Human Herpesvirus ◽  
Neutralizing Antibody ◽  
Signs And Symptoms ◽  
Human Herpesvirus 6 ◽  
Exanthem Subitum ◽  
Herpesvirus 6

Objective. To clarify clinical features of patients with primary human herpesvirus 6 (HHV-6) infection (roseola infantum, exanthem subitum) in a large-scale study. Subjects and methods. Clinical signs and symptoms were analyzed in 176 infants in whom exanthem subitum was initially suspected and primary HHV-6 infection was later confirmed. The infection was proved by isolation of the virus from blood, a significant increase in the neutralizing antibody titers to the virus, or both. Results. The primary HHV-6 infection, which occurred throughout the year, was observed in 94 boys and 82 girls (mean age, 7.3 months). Fever developed in 98% (mean maximum fever, 39.4°C) and lasted for 4.1 days. Macular or papular rashes appeared in 98%, on face, trunk, or both, mostly at the time of subsidence of the fever, and lasted for 3.8 days. Other clinical manifestations occurred as follows: mild diarrhea in 68%, edematous eyelids in 30%, erythematous papules in the pharynx in 65%, cough in 50%, and mild cervical lymph node swelling in 31%. Twenty-six percent had bulging of the anterior fontanelle and 8% had convulsions. Conclusions. Clinical features of patients with virologically confirmed exanthem subitum were comparable with those described before discovery of HHV-6.

scholarly journals Inherited chromosomally integrated human herpesvirus 6 as a predisposing risk factor for the development of angina pectoris

2015 ◽  
Vol 112 (26) ◽  
pp. 8058-8063 ◽  
Author(s):  
Annie Gravel ◽  
Isabelle Dubuc ◽  
Guillaume Morissette ◽  
Ruth H. Sedlak ◽  
Keith R. Jerome ◽  
...  
Keyword(s):  
Risk Factor ◽  
Angina Pectoris ◽  
Large Scale ◽  
Germ Line ◽  
Human Herpesvirus ◽  
Bonferroni Correction ◽  
Human Herpesvirus 6 ◽  
Large Scale Analysis ◽  
Herpesvirus 6 ◽  
Germ Line Transmission

Inherited chromosomally integrated human herpesvirus-6 (iciHHV-6) results in the germ-line transmission of the HHV-6 genome. Every somatic cell of iciHHV-6+ individuals contains the HHV-6 genome integrated in the telomere of chromosomes. Whether having iciHHV-6 predisposes humans to diseases remains undefined. DNA from 19,597 participants between 40 and 69 years of age were analyzed by quantitative PCR (qPCR) for the presence of iciHHV-6. Telomere lengths were determined by qPCR. Medical records, hematological, biochemical, and anthropometric measurements and telomere lengths were compared between iciHHV-6+ and iciHHV-6− subjects. The prevalence of iciHHV-6 was 0.58%. Two-way ANOVA with a Holm–Bonferroni correction was used to determine the effects of iciHHV6, sex, and their interaction on continuous outcomes. Two-way logistic regression with a Holm–Bonferroni correction was used to determine the effects of iciHHV6, sex, and their interaction on disease prevalence. Of 50 diseases monitored, a single one, angina pectoris, is significantly elevated (3.3×) in iciHHV-6+ individuals relative to iciHHV-6− subjects (P = 0.017; 95% CI, 1.73–6.35). When adjusted for potential confounding factors (age, body mass index, percent body fat, and systolic blood pressure), the prevalence of angina remained three times greater in iciHHV-6+ subjects (P = 0.015; 95%CI, 1.23–7.15). Analyses of telomere lengths between iciHHV-6− without angina, iciHHV-6− with angina, and iciHHV-6+ with angina indicate that iciHHV-6+ with angina have shorter telomeres than age-matched iciHHV-6− subjects (P = 0.006). Our study represents, to our knowledge, the first large-scale analysis of disease association with iciHHV-6. Our results are consistent with iciHHV-6 representing a risk factor for the development of angina.

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