scholarly journals Comparison of Selenium Determination in Liver Samples by Atomic Absorption Spectroscopy and Inductively Coupled Plasma–Mass Spectrometry

2005 ◽  
Vol 17 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Irina Rudik Miksa ◽  
Carol L. Buckley ◽  
Nancy P. Carpenter ◽  
Robert H. Poppenga
Keyword(s):  
Mass Spectrometry ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Magnesium Nitrate ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Selenium (Se) is an essential trace element that is often deficient in the natural diets of domestic animal species. The measurement of Se in whole blood or liver is the most accurate way to assess Se status for diagnostic purposes. This study was conducted to compare hydride generation atomic absorption spectroscopy (HG-AAS) with inductively coupled plasma–mass spectrometry (ICP-MS) for the detection and quantification of Se in liver samples. Sample digestion was accomplished with magnesium nitrate and nitric acid for HG-AAS and ICP-MS, respectively. The ICP-MS detection was optimized for 82Se with yttrium used as the internal standard and resulted in a method detection limit of 0.12 μg/g. Selenium was quantified by both methods in 310 samples from a variety of species that were submitted to the Toxicology Laboratory at New Bolton Center (Kennett Square, PA) for routine diagnostic testing. Paired measurements for each sample were evaluated by a mean difference plot method. Limits of agreement were used to describe the maximum differences likely to occur between the 2 methods. Results suggest that under the specified conditions ICP-MS can be reliably used in place of AAS for quantitation of tissue Se at or below 2 μg/g to differentiate between adequate and deficient liver Se concentrations.

scholarly journals Method for measurement of serum copper, zinc and selenium using total reflection X-ray fluorescence spectroscopy on the PICOFOX analyser: Validation and comparison with atomic absorption spectroscopy and inductively coupled plasma mass spectrometry

2018 ◽  
Vol 56 (1) ◽  
pp. 170-178 ◽  
Author(s):  
Jinny Jeffery ◽  
Amy R Frank ◽  
Stephanie Hockridge ◽  
Hagen Stosnach ◽  
Seán J Costelloe
Keyword(s):  
Mass Spectrometry ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Inductively Coupled Plasma ◽  
Total Reflection ◽  
X Ray ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Cu And Zn

Background Total reflection X-ray fluorescence is a comparably new method for the measurement of trace elements in biological samples. Methods Results obtained by total reflection X-ray fluorescence were compared to atomic absorption spectroscopy for Cu and Zn and inductively coupled plasma mass spectrometry for Cu, Zn and Se in patient serum. The total reflection X-ray fluorescence assay was characterized for accuracy; recovery; intra- and inter-assay imprecision (using patients’ samples, external quality assurance and quality control materials); limit of blank; limit of detection; linearity; interference and stability of prepared samples. Results Minimal sample preparation is required for total reflection X-ray fluorescence and simultaneous multi-elemental analysis is possible in clinical samples. There was a small positive bias for Cu and Zn measurements using total reflection X-ray fluorescence compared to atomic absorption spectroscopy and inductively coupled plasma mass spectrometry and a significant negative bias for Se measurements by total reflection X-ray fluorescence relative to inductively coupled plasma mass spectrometry. Recovery, imprecision and linearity were acceptable. The limit of detection was shown to be 1.2  μmol/L for serum Cu, 1.8  μmol/L for serum Zn and 0.2  μmol/L for serum Se. Conclusions Measurement of Cu and Zn in serum samples using total reflection X-ray fluorescence would be a viable alternative to atomic absorption spectroscopy or inductively coupled plasma mass spectrometry. The volatility of some Se compounds results in lower Se results being reported using total reflection X-ray fluorescence and further work would be necessary to identify whether total reflection X-ray fluorescence has an acceptable clinical sensitivity and specificity for the assessment of Se deficiency. Measurement of copper, zinc and selenium on whole blood samples is possible using total reflection X-ray fluorescence which may provide a more accurate assessment of trace element deficiency for patients with an acute phase response.

Effects of mercury and thallium concentrations on high precision determination of mercury isotopic composition by Neptune Plus multiple collector inductively coupled plasma mass spectrometry

2016 ◽  
Vol 31 (10) ◽  
pp. 2060-2068 ◽  
Author(s):  
Runsheng Yin ◽  
David P. Krabbenhoft ◽  
Bridget A. Bergquist ◽  
Wang Zheng ◽  
Ryan F. Lepak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Precision ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Inductively Coupled ◽  
Precision Determination ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
High Precision Determination

Thallium (Tl) has been widely used as an internal standard for mass bias correction during high precision mercury (Hg) isotope ratio measurements using multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS).

A Study of Internal Standardization in Inductively Coupled Plasma-Mass Spectrometry

1987 ◽  
Vol 41 (5) ◽  
pp. 801-806 ◽  
Author(s):  
Joseph J. Thompson ◽  
R. S. Houk
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Operating Conditions ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Ionization Suppression ◽  
Plasma Mass Spectrometry ◽  
Internal Standardization ◽  
Very High

Internal standardization is employed to compensate for ionization suppression in inductively coupled plasma-mass spectrometry (ICP-MS). By examination of the response of over 50 elements to a sodium matrix under different operating conditions, it is apparent that an internal standard is most effective when it is close in mass and ionization energy to the analyte. The extent of suppression and the relative order of suppression of various analyte elements can differ for various matrix elements. Generally, precision was improved by the use of internal standardization; the extent of improvement differed for different analyte elements and operating conditions. A comparison between ICP-MS with ultrasonic and pneumatic nebulization is described. The ultrasonic nebulizer usually exhibits better sensitivity and detection limits for analyte elements, unless the extent of suppression induced by the concomitant matrix is very high.

A candidate reference method for serum potassium measurement by inductively coupled plasma mass spectrometry

2017 ◽  
Vol 55 (10) ◽  
Author(s):  
Ying Yan ◽  
Bingqing Han ◽  
Jie Zeng ◽  
Weiyan Zhou ◽  
Tianjiao Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Serum Potassium ◽  
Inductively Coupled Plasma ◽  
Low Cost ◽  
Internal Standard ◽  
Reference Method ◽  
Serum Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

AbstractBackground:Potassium is an important serum ion that is frequently assayed in clinical laboratories. Quality assurance requires reference methods; thus, the establishment of a candidate reference method for serum potassium measurements is important.Methods:An inductively coupled plasma mass spectrometry (ICP-MS) method was developed. Serum samples were gravimetrically spiked with an aluminum internal standard, digested with 69% ultrapure nitric acid, and diluted to the required concentration. TheResults:The correlation coefficients between the measuredConclusions:The new ICP-MS method is specific, precise, simple, and low-cost, and it may be used as a candidate reference method for standardizing serum potassium measurements.

scholarly journals Multielemental Analysis of Food and Agricultural Matrixes by Inductively Coupled Plasma-Mass Spectrometry

2002 ◽  
Vol 85 (1) ◽  
pp. 113-121 ◽  
Author(s):  
Francesco Cubadda ◽  
Andrea Andrea ◽  
Antonella Testoni ◽  
Fabio Fabio
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Internal Standard ◽  
Multielemental Analysis ◽  
Inductively Coupled ◽  
Analytical Protocol ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Staple Foods ◽  
Correction Equations

Abstract The suitability of quadrupole inductively coupled plasma-mass spectrometry (ICP-MS) for multielemental analysis of food following microwave closed vessel digestion of samples was evaluated in relation to analytical challenges presented by some major food and agricultural matrixes. Fifteen key analytes (Al, As, Cd, Co, Cr, Cu, Fe, Mn, Mo, Ni, Pb, Se, Sn, V, Zn) were determined in 9 reference materials representative of 3 major groups of staple foods (cereals, seafood, and meat). For all measurements, the method of external calibration was used and Rh was selected as internal standard. Matrix-induced interferences were evaluated for each material, and suitable methods to overcome them were applied. Spectral interferences caused by C, Cl, and Ca content of digestates were quantitated and corrected by entering a simple system of mathematical correction equations into the instrument software before each analytical run. Full mastering of interferences together with adoption of a series of measures to control the reliability of analytical measurements produced accurate results for all the analytes. A standard analytical protocol is outlined for the benefit of both research and routine high-throughput laboratories that perform ICP-MS analyses of food.

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