scholarly journals Evaluation of a PCR assay on overgrown environmental samples cultured for Mycobacterium avium subsp. paratuberculosis

2016 ◽  
Vol 28 (6) ◽  
pp. 638-645 ◽  
Author(s):  
Juan C. Arango-Sabogal ◽  
Olivia Labrecque ◽  
Julie Paré ◽  
Julie-Hélène Fairbrother ◽  
Jean-Philippe Roy ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Environmental Samples ◽  
Mycobacterium Avium Subsp ◽  
Pcr Assay ◽  
Mycobacterium Avium Subsp Paratuberculosis

Evaluation of a PCR assay on overgrown individual fecal samples cultured for Mycobacterium avium subsp. paratuberculosis

2017 ◽  
Vol 29 (6) ◽  
pp. 912-915 ◽  
Author(s):  
Saray Rangel ◽  
Juan C. Arango-Sabogal ◽  
Olivia Labrecque ◽  
Julie Paré ◽  
Julie-Hélène Fairbrother ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Pcr Assay ◽  
Fecal Samples ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals High-Throughput Direct Fecal PCR Assay for Detection of Mycobacterium avium subsp. paratuberculosis in Sheep and Cattle

2013 ◽  
Vol 52 (3) ◽  
pp. 745-757 ◽  
Author(s):  
K. M. Plain ◽  
I. B. Marsh ◽  
A. M. Waldron ◽  
F. Galea ◽  
A.-M. Whittington ◽  
...  
Keyword(s):  
High Throughput ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Pcr Assay ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Presence of Mycobacterium avium subsp. paratuberculosis in Environmental Samples Collected on Commercial Dutch Dairy Farms

2010 ◽  
Vol 76 (18) ◽  
pp. 6310-6312 ◽  
Author(s):  
Susanne W. F. Eisenberg ◽  
Ad P. Koets ◽  
Jeroen Hoeboer ◽  
Marina Bouman ◽  
Dick Heederik ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Causative Agent ◽  
Environmental Samples ◽  
Dairy Farms ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mycobacterium Avium Subsp ◽  
Young Stock ◽  
Settled Dust ◽  
Mycobacterium Avium Subsp Paratuberculosis

ABSTRACT Mycobacterium avium subsp. paratuberculosis, the causative agent of Johne's disease in cattle, was identified in settled-dust samples of Dutch commercial dairy farms, both in the dairy barn and in the young stock housing. Bioaerosols may play a role in within-farm M. avium subsp. paratuberculosis transmission.

Quantification of Mycobacterium avium subsp. paratuberculosis from the tissues of challenged mice using SYBR Green real time PCR assay for the assessment of vaccine efficacy

2018 ◽  
Author(s):  
R. K. Chaitanya ◽  
Y.Krishnamohan Reddy ◽  
G.Dhinakar Raj ◽  
A. Thangavelu
Keyword(s):  
Real Time ◽  
Mycobacterium Avium ◽  
Real Time Pcr ◽  
Protective Efficacy ◽  
Absolute Quantification ◽  
Sybr Green ◽  
Mycobacterium Avium Subsp ◽  
Bacterial Burden ◽  
Pcr Assay ◽  
Mycobacterium Avium Subsp Paratuberculosis

A SYBR Green real time PCR assay amplifying F57 gene of Mycobacterium avium subsp. paratuberculosis (MAP) was developed to evaluate the protective efficacy of the inactivated vaccine to prevent the colonization of MAP in the tissues of BALB/c mice challenge model. Bacterial burden in the liver, spleen and intestine of vaccinated and sham immunized control mice, after intra peritoneal challenge with MAP were quantified by real time PCR assay. A 195 bp fragment of MAP F57 sequence was cloned into TA cloning vector and the resultant recombinant plasmid was used to generate a series of quantification standards with copy number ranging from 109 to 1 copy. This absolute quantification technique is rapid and able to detect as few as 10 MAP organisms per gram of tissue and the assay has the efficiency of 0.982.

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