Deregulated MicroRNA Signature Following Glioblastoma Irradiation

Glioblastoma (GBM), the most common and aggressive brain tumor in adults, shows resistance to treatment, particularly radiotherapy. One method for effective treatment is using a group of radiosensitizers that make tumor cells responsive to radiotherapy. A class of molecules whose expression is affected by radiotherapy is the microRNAs (miRNAs) that present promising regulators of the radioresponse. Eighteen miRNAs (miR-26a, -124, -128, -135b, -145, -153, -181a/b, -203, -21, -210, -212, -221/222, -223, -224, -320, and -590), involved in the pathogenesis of GBM and its radioresponsive state, were reviewed to identify their role in GBM and their potential as radiosensitizing agents. MicroRNAs-26a, -124, -128, -145, -153, -181a/b, -203, -221/222, -223, -224, -320, and -590 promoted GBM radiosensitivity, while microRNAs-135b, -21, -210, and -212 encouraged radioresistance. Ectopic overexpression of the radiosensitivity promoting miRNAs and knockdown of the radioresistant miRNAs represent a prospective radiotherapy enhancement opportunity. This offers a glimmer of hope for a group of the most unfortunate patients known to medicine.

Download Full-text

Synthetic scorpion venom targets brain tumor cells

PsycEXTRA Dataset ◽

10.1037/e458452008-008 ◽

2006 ◽

Keyword(s):

Brain Tumor ◽

Tumor Cells ◽

Scorpion Venom ◽

Brain Tumor Cells

Download Full-text

DIPG-60. PILOT STUDY OF CIRCULATING TUMOR CELLS IN PEDIATRIC HIGH GRADE BRAIN TUMORS

Neuro-Oncology ◽

10.1093/neuonc/noaa222.105 ◽

2020 ◽

Vol 22 (Supplement_3) ◽

pp. iii299-iii299

Author(s):

Wafik Zaky ◽

Long Dao ◽

Dristhi Ragoonanan ◽

Izhar Bath ◽

Sofia Yi ◽

...

Keyword(s):

Brain Tumor ◽

Brain Tumors ◽

Tumor Cells ◽

Circulating Tumor Cells ◽

Pediatric Brain Tumor ◽

Pediatric Brain Tumors ◽

Disease Assessment ◽

Liquid Biopsies ◽

Capture Method ◽

Pediatric Brain

Abstract BACKGROUND Despite its increasing use, circulating tumor cells (CTCs) have not been studied in pediatric brain tumors. METHODS Cell surface vimentin (CSV) is a marker for CTC detection. We developed an automated CSV-based CTC capture method for pediatric brain tumor using the Abnova Cytoquest platform. PBMCs isolated from blood samples from 52 brain tumor patients were processed to isolate CSV+ CTCs. Captured cells were then stained for CSV and CD45 and scanned to determine the number of CTCs. DIPG samples were additionally examined for H3K27M expression on CSV+ cells. Long term cancer survivors were used as a control cohort. RESULTS 86.4% of all the samples exhibited between 1–13 CSV+ CTCs, with a median of 2 CSV+ CTCs per sample. Using a value of ≥ 1 CTC as a positive result, the sensitivity and specificity of this test was 83.05% and 60.0% respectively. 19 DIPG samples were analyzed and 70% (13 samples) were positive for 1–5 CTCs. Five of these 7 positive CSV+ CTCs DIPG samples were also positive for H3K27M mutations by immunohistochemistry (71%). Mean survival in days for the CTC positive and negative DIPG samples were 114 and 211 days, respectively (p= 0.13). CONCLUSION This is the first study of CTCs in pediatric CNS tumors using an automated approach. Patients with brain tumors can exhibit CSV+ CTCs within peripheral blood. The use of specific molecular markers such as H3K27M can improve the diagnostic capability of liquid biopsies and may enable future disease assessment for personalized therapy.

Download Full-text

Synchronization of 9L rat brain tumor cells by centrifugal elutriation

Cell Biophysics ◽

10.1007/bf02790449 ◽

1980 ◽

Vol 2 (3) ◽

pp. 191-206 ◽

Cited By ~ 32

Author(s):

P. C. Keng ◽

C. K. N. Li ◽

K. T. Wheeler

Keyword(s):

Brain Tumor ◽

Rat Brain ◽

Tumor Cells ◽

Centrifugal Elutriation ◽

Brain Tumor Cells ◽

Rat Brain Tumor

Download Full-text

Development and estimation of brain tumor cells culture flask with acoustic window film for ultrasound exposure

Japanese Journal of Applied Physics ◽

10.7567/jjap.53.07kf12 ◽

2014 ◽

Vol 53 (7S) ◽

pp. 07KF12 ◽

Cited By ~ 1

Author(s):

Sakino Iwasiro ◽

Akiko Watanabe ◽

Yoshifumi Onozuka ◽

Masatsune Minai ◽

Hiroyuki Nishimura ◽

...

Keyword(s):

Brain Tumor ◽

Tumor Cells ◽

Ultrasound Exposure ◽

Acoustic Window ◽

Culture Flask ◽

Brain Tumor Cells ◽

Window Film

Download Full-text

BCNU-modification of the in vitro radiation response in 9L brain tumor cells of rats

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/0360-3016(77)90011-6 ◽

1977 ◽

Vol 2 (1-2) ◽

pp. 79-88 ◽

Cited By ~ 36

Author(s):

Kenneth T. Wheeler ◽

Dennis F. Deen ◽

Charles B. Wilson ◽

Mary E. Williams ◽

Sandra Sheppard

Keyword(s):

Brain Tumor ◽

Tumor Cells ◽

Radiation Response ◽

Brain Tumor Cells

Download Full-text

Elevation of osteopontin levels in brain tumor cells reduces burden and promotes survival through the inhibition of cell dispersal

Journal of Neuro-Oncology ◽

10.1007/s11060-007-9477-1 ◽

2007 ◽

Vol 86 (3) ◽

pp. 285-296 ◽

Cited By ~ 8

Author(s):

Stephen M. Selkirk ◽

Jay Morrow ◽

Tara A. Barone ◽

Alan Hoffer ◽

Jeffrey Lock ◽

...

Keyword(s):

Brain Tumor ◽

Tumor Cells ◽

Brain Tumor Cells

Download Full-text

Production of matrix metalloproteinases and tissue inhibitor of metalloproteinases-1 by human brain tumors

Journal of Neurosurgery ◽

10.3171/jns.1994.81.1.0069 ◽

1994 ◽

Vol 81 (1) ◽

pp. 69-77 ◽

Cited By ~ 133

Author(s):

Takao Nakagawa ◽

Toshihiko Kubota ◽

Masanori Kabuto ◽

Kazufumi Sato ◽

Hirokazu Kawano ◽

...

Keyword(s):

Brain Tumor ◽

Matrix Metalloproteinases ◽

Brain Tumors ◽

Human Brain ◽

Tumor Cells ◽

Tumor Invasion ◽

Gelatinolytic Activity ◽

Normal Brain ◽

Tissue Inhibitor Of Metalloproteinases ◽

Human Brain Tumor

✓ The role of matrix metalloproteinases (MMP's) and their inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), in human brain tumor invasion was investigated. Gelatinolytic activity was assayed via gelatin zymography, and four MMP's (MMP-1, MMP-2, MMP-3, and MMP-9) and TIMP-1 were immunolocalized in human brain tumors and in normal brain tissues using monoclonal antibodies. The tissue was surgically removed from 44 patients: glioblastoma (five cases), anaplastic astrocytoma (six cases), astrocytoma (four cases), metastatic tumor (six cases), neurinoma (10 cases), meningioma (10 cases), and normal brain tissue (three cases). Glioblastomas, anaplastic astrocytomas, and metastatic tumors showed high gelatinolytic activity and positive immunostaining for MMP's; TIMP-1 was also expressed in these tumors, but some tumor cells were negative for the antibody. Astrocytomas had low gelatinolytic activity and the tumor cells showed no immunoreactivity for MMP's and TIMP-1. Although neurinomas and meningiomas had only moderate proteinase activity and exhibited positive immunoreactivity for MMP-9, intense expression of TIMP-1 was simultaneously observed in these tumor cells. These findings suggest that MMP's play an important role in human brain tumor invasion, probably due to an imbalance between the production of MMP's and TIMP-1 by the tumor cells.

Download Full-text