Bioactive Composition of Pig Laryngeal Cartilage Extracts and Their Free                 Radical-scavenging Activity

This work focused on amino acid and mineral element analysis of pig laryngeal cartilage. Papain and DEAE-Sepharose fast flow ion exchange chromatography were used to hydrolyase cartilage and purify hydrolysate. Further study was performed to compare the free radical-scavenging activity of various fractions. Cartilage is abundant in bioactive amino acids such as glycine, glutamic acid and arginine and minerals such as calcium, magnesium and iron. Four polysaccahrides attached peptides and one peptide were isolated from hydrolysate. Weak 2,2-diphenyl-1-picryhydrazyl free radical scavenging activities of all substances were observed. Other fractions showed hydroxyl free radical and superoxide anion free radical scavenging ability in a concentration-dependent manner except fraction A. The hydroxyl free radical scavenging activity of tested samples decreased in the following order: fraction C crude chondroitin sulphate (ChS) peptide fraction B semi-purified ChS purified ChS. Significant differences were observed between purified ChS and other fractions (P 0.01). For superoxide anion free radical the order was: peptide purified ChS semi-purified ChS crude ChS fraction C fraction B. The peptide was significantly higher than purified ChS (P 0.01), which was significantly higher than the other components (P 0.01). According to the results, polysaccharides, ChS, amino acid and peptide present in cartilage had important bioactivity.

Download Full-text

Note: A Comparative Study on the in Vitro Antiradical Activity and Hydroxyl Free Radical Scavenging Activity in Aged Red Wines

Food Science and Technology International ◽

10.1177/1082013203040080 ◽

2003 ◽

Vol 9 (6) ◽

pp. 383-387 ◽

Cited By ~ 19

Author(s):

P. Kefalas ◽

S. Kallithraka ◽

I. Parejo ◽

D. P. Makris

Keyword(s):

Free Radical ◽

Antiradical Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Red Wines ◽

Hydroxyl Free Radical ◽

Important Relationship

The antiradical and hydroxyl free radical scavenging activities were estimated in twenty-five, aged red wines from different areas in Greece. The antiradical activity (AAR) was determined by means of the wellknown DPPH• method, and its values ranged from 24.7 to 125.1. A novel, chemiluminescence-based, highly sensitive assay was applied for determination of the hydroxyl free radical scavenging activity (SAHFR), which varied from 1.62 to 12.22 mM quercetin equivalents. The values from the two assays correlated very well (r2 =0.8542, P<0.001), which confirmed an important relationship between SAHFR and AAR. This tendency in aged red wines, which may be significant in evaluating the antioxidant behaviour of red wine polyphenols, is discussed on the basis of previous research and relevant data.

Download Full-text

Potent Free Radical Scavenging Activity of Propol Isolated from Brazilian Propolis

Zeitschrift für Naturforschung C ◽

10.1515/znc-1997-11-1217 ◽

1997 ◽

Vol 52 (11-12) ◽

pp. 828-833 ◽

Cited By ~ 34

Author(s):

Purusotam Basnet ◽

Tetsuya Matsuno ◽

Richard Neidlein

Keyword(s):

Free Radical ◽

Vitamin E ◽

Superoxide Anion ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Water Extract ◽

Free Radical Scavenging ◽

Free Radical Scavenging Activity ◽

Scavenging Activity ◽

Brazilian Propolis

Abstract We evaluated free radical scavenging activity of the water, methanol and chloroform extracts of propolis in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and xanthine-xanthine oxidase (XOD) generated superoxide anion assay systems. The free radical scavenging activity guided fractionation and chemical analysis led to the isolation of a new compound, propol {3-[4-hydroxy-3-(3-oxo-but-1-enyl)-phenyl]-acrylic acid) from the water extract, which was more potent than most common antioxidants such as vitamin C and vitamin E (α-tocopherol) in these assay systems.

Download Full-text