Wide-field individual retinal layer thickness in healthy eyes

2020 ◽  
pp. 112067212092766
Author(s):  
Sumit Randhir Singh ◽  
Alessandro Invernizzi ◽  
Mohammed Abdul Rasheed ◽  
Carlo Cagini ◽  
Abhilash Goud ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Regression Analysis ◽  
Layer Thickness ◽  
Healthy Subjects ◽  
Retinal Thickness ◽  
Optic Disk ◽  
Retinal Layer ◽  
Wide Field ◽  
Optical Coherence ◽  
Healthy Eyes

Purpose To report the individual retinal layer thickness in healthy subjects using wide-field optical coherence tomography. Methods This was a prospective, cross-sectional study involving healthy subjects. A custom-designed semiautomated segmentation algorithm was used to split the retinal layers in seven bands, and individual retinal layer thicknesses were measured in horizontal (nasal, macular, and temporal segments) and vertical meridians (superior, macular, and inferior segments). The variation in retinal thickness was analyzed in different segments at an interval of 1 mm from reference points. Regression analysis was performed to identify the factors affecting retinal thickness. Results Twenty eyes of 20 healthy subjects with mean age of 28.9 ± 6.3 years were analyzed. Overall, nasal and superior segments (mean ± standard deviation: 279.6 ± 17.0 and 234.4 ± 19.2 µm) had maximum and minimum retinal thicknesses, respectively. A total of seven bands were delineated in each optical coherence tomography b scan in each segment. Retinal nerve fiber layer was thickest immediately nasal to optic disk margin in horizontal scan (72.4 ± 32.4 µm) and near the vascular arcades in vertical meridian. Outer plexiform layer, external limiting membrane–ellipsoid zone and interdigitation zone–retinal pigment epithelium–Bruch’s complex showed significant variation in both horizontal and vertical meridians (all p values <0.05). Macular segment in both meridians showed the highest coefficient of variation. Age was the only significant factor affecting retinal thickness in multiple regression analysis (p = 0.001). Conclusions Wide-field optical coherence tomography shows significant regional variation in overall and individual retinal layer thicknesses in macular and peripheral areas in healthy eyes with the highest variation in macular segment.

Wide-field optical coherence tomography imaging in diabetic retinopathy

2021 ◽  
pp. 112067212110549
Author(s):  
Ilkay Kilic Muftuoglu ◽  
Gagan Kalra ◽  
Mohammed Abdul Rasheed ◽  
Carlo Cagini ◽  
Ramkailash Gujar ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Healthy Subjects ◽  
Outer Nuclear Layer ◽  
Plexiform Layer ◽  
Reference Points ◽  
Retinal Layer ◽  
Wide Field ◽  
Inner Plexiform Layer ◽  
Optical Coherence

Purpose To report the individual retinal layer thicknesses up to mid-equator in patients with diabetic retinopathy (DR) using Spectralis (Heidelberg Engineering, Heidelberg, Germany) wide-field optical coherence tomography (OCT). Methods Retinal layers were segmented using a custom designed semi-automated algorithm, where reference points were marked by the examiner to enable software to automatically compute the thickness values of each retinal sublayer at an interval of 1 mm from reference points. The values of individual retinal thicknesses in eyes with varying severity of DR were compared with the values of healthy subjects. Generalized estimating equation was performed to compensate for inclusion of both eyes of patients. Results A total of 64 patients (119 eyes) with a mean age of 68.97  ±  10.27 years were included. Overall, ganglion cell layer (GCL)/ inner plexiform layer (IPL) complex (-31.67 microns, p < 0.001), outer plexiform layer (-6.78 microns, p = 0.002) and photoreceptor layer (-22.90 microns, p < 0.001) showed significant thinning, while outer nuclear layer thickening ( + 68.19 microns, <0.001) was noted in eyes with DM compared to healthy subjects. Thickness changes were significantly more in the macular segment compared to nasal and temporal segments. GCL/ IPL complex and photoreceptor layers were found to be significantly thin in all grades of DR. Conclusion Retinal thicknesses vary significantly in patients with diabetic retinopathy and understanding patterns of these changes across different segments of the wide field OCT may help better elucidate the natural progression of the disease in terms of retinal anatomy.

scholarly journals Assessment of Global and Local Alterations in Retinal Layer Thickness in Ins2 (Akita) Diabetic Mice by Spectral Domain Optical Coherence Tomography

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Andrew W. Francis ◽  
Justin Wanek ◽  
Mahnaz Shahidi
Keyword(s):  
Optical Coherence Tomography ◽  
Mouse Model ◽  
Layer Thickness ◽  
Retinal Thickness ◽  
Normative Values ◽  
Retinal Layer ◽  
Wild Type ◽  
Global And Local ◽  
Akita Mice ◽  
Sd Oct

Purpose/Aim. The Ins2 (Akita) mouse is a spontaneous diabetic mouse model with a heterozygous mutation in the insulin 2 gene that results in sustained hyperglycemia. The purpose of the study was to assess global and local retinal layer thickness alterations in Akita mice by analysis of spectral domain optical coherence tomography (SD-OCT) images.Materials and Methods. SD-OCT imaging was performed in Akita and wild-type mice at 12 and 24 weeks of age. Inner retinal thickness (IRT), outer retinal thickness (ORT), total retinal thickness (TRT), and photoreceptor outer segment length (OSL) were measured. Mean global thickness values were compared between Akita and wild-type mice. Local thickness variations in Akita mice were assessed based on normative values in wild-type mice.Results. Akita mice had higher blood glucose levels and lower body weights (p<0.001). On average, IRT, ORT, and TRT were approximately 2% lower in Akita mice than in wild-type mice (p≤0.02). In Akita mice, the percent difference between retinal areas with thickness below and above normative values for IRT, ORT, and TRT was 22%, 32%, and 38%, respectively.Conclusions. These findings support the use of the Akita mouse model to study the retinal neurodegenerative effects of hyperglycemia.

scholarly journals Alterations in Retinal Layer Thickness and Reflectance at Different Stages of Diabetic Retinopathy by En Face Optical Coherence Tomography

2016 ◽  
Vol 57 (9) ◽  
pp. OCT341 ◽  
Author(s):  
Justin Wanek ◽  
Norman P. Blair ◽  
Felix Y. Chau ◽  
Jennifer I. Lim ◽  
Yannek I. Leiderman ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diabetic Retinopathy ◽  
Layer Thickness ◽  
Retinal Layer ◽  
Optical Coherence ◽  
En Face

Wide-field optical coherence tomography deviation map for early glaucoma detection

2021 ◽  
pp. bjophthalmol-2021-319509
Author(s):  
Hyungjun Kim ◽  
Hae Min Park ◽  
Hyo Chan Jeong ◽  
So Yeon Moon ◽  
Hyunsoo Cho ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Characteristic Curve ◽  
Wide Field ◽  
Optical Coherence ◽  
Diagnostic Ability ◽  
Swept Source ◽  
Early Glaucoma ◽  
Field Database ◽  
The Individual ◽  
Healthy Eyes

Background/aimsThis study aimed to establish a wide-field optical coherence tomography (OCT) deviation map obtained from swept-source OCT (SS-OCT) scans. Moreover, it also aimed to compare the diagnostic ability of this wide-field deviation map with that of the peripapillary and macular deviation maps currently being used for the detection of early glaucoma (EG).MethodsFour hundred eyes, including 200 healthy eyes and 200 eyes with EG were enrolled in this retrospective observational study. Patients underwent a comprehensive ocular examination, including wide-field SS-OCT (DRI-OCT Triton; Topcon, Tokyo, Japan). The individual wide-field scan was converted into a uniform template using the fovea and optic disc centres as fixed landmarks. Subsequently, the wide-field deviation map was obtained via the comparison between individual wide-field data and a normative wide-field database that had been created by combining images of healthy eyes into a uniform template in a previous study. The ability of the new wide-field deviation map to distinguish between EG and healthy eyes was assessed by comparing it with conventional deviation maps based on the area under the receiver operating characteristic curve (AUC).ResultsThe wide-field deviation map obtained using the normative wide-field database showed the highest diagnostic ability for the diagnosis of EG (AUC=0.980 and 961 for colour-coded pixels presenting <5% and <1%, respectively) among various deviation maps. Its AUC was significantly superior to that of most conventional deviation maps (p<0.05). The wide-field deviation map demonstrated early structural glaucomatous damage well over a wider area.ConclusionThe wide-field SS-OCT deviation map exhibited good performance for distinguishing between eyes with EG and healthy eyes. The visualisation of the wider damaged area on the wide-field deviation map could be useful for the diagnosis of EG in clinical settings.

scholarly journals Comparison of Superficial and Deep Foveal Avascular Zone Area in Healthy Subjects Using Two Spectral Domain Optical Coherence Tomography Angiography Devices

2020 ◽  
Author(s):  
Pasha Anvari ◽  
Amin Najafi ◽  
Reza Mirshahi ◽  
Mahsa Sardarinia ◽  
Maryam Ashrafkhorasani ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Healthy Subjects ◽  
Optical Coherence Tomography Angiography ◽  
Spectral Domain ◽  
Foveal Avascular Zone ◽  
Retinal Layer ◽  
Optical Coherence ◽  
Retinal Layers ◽  
Avascular Zone ◽  
The Mean

Purpose: To compare the area of the foveal avascular zone (FAZ) in the superficial and deep retinal layers using two different spectral-domain optical coherence tomography angiography (OCTA) devices. Methods: A cross-sectional comparative study was conducted to obtain macular OCTA images from healthy subjects using Optovue RTVue XR Avanti (Optovue, Inc, Fremont, CA) and Spectralis HRA+OCTA (Heidelberg Engineering, Heidelberg, Germany). Two independent trained graders measured the FAZ area using automated slab segmentation. The FAZ area in the superficial and deep retinal layers were compared. Results: Twenty-three eyes of 23 subjects were included. The graders agreement was excellent (>0.86) for all measurements. The mean FAZ area was significantly larger at the superficial retinal layer as compared to the deep retinal layer on both devices (0.31 ± 0.08 mm2 vs 0.26 ± 0.08 mm2 in Optovue and 0.55 ± 0.16 mm2 vs 0.36 ± 0.13 mm2 in Spectralis, both P < 0.001). The mean FAZ area was significantly greater in the superficial and deep retinal layers using Spectralis as compared to Optovue measurements (P < 0.001 for both comparisons). Conclusion: In contrast to previous reports, the FAZ area was larger in the superficial retina as compared to deep retinal layers using updated software versions. Measurements from different devices cannot be used interchangeably.

Sign in / Sign up

Export Citation Format

Share Document