MORPHOLOGICAL AND CYTOCHEMICAL PROPERTIES OF THE HOLOCRINE CELLS IN THE EPIDIDYMIS OF THE MOUSE

Holocrine secretory cells have been identified in the epithelium of the epididymal canal of the mouse. These cells develop from basal cells. During their differentiation they grow toward the lumen of the epididymal canal and come to form club-shaped structures with an expanded apical portion, a central nucleus and a thin stalk-like connection to the basement membrane. Mature holocrine cells are characterized by their high acid phosphatase and aliesterase activity. They also are highly active for succinic dehydrogenase, nicotinamide adenine dinucleotide diaphorase, and nicotinamide adenine dinucleotide phosphate diaphorase. Nucleoside diphosphatase, thiamine pyrophosphatase, adenosine triphosphatase, and alkaline nucleoside phosphatase are also found in these cells. These cells are also characterized by their reactivity with the Aoyama and periodic acid-Schiff reactions. They react moderately with the molybdate and Luxol Fast Blue MBS reactions for choline containing compounds. Mature holocrine cells may disintegrate in situ or may be discharged in toto into the lumen of the epididymal canal. Glycerylphosphorylcholine was identified in extracts prepared from sperm-free epididymides of mice. Glycerylphosphorylcholine reacts with Aoyama and periodic acid-Schiff reactions as do mature holocrine cells. This fact coupled with the identification of choline containing material in holocrine cells suggests that they may be one site for the formation of glycerylphosphorylcholine.

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Structural and Ultrastructural Morphological Evaluation of Giant Anteater (Myrmecophaga tridactyla) Prostate Gland

Biology ◽

10.3390/biology10030231 ◽

2021 ◽

Vol 10 (3) ◽

pp. 231

Author(s):

Fernanda Moura ◽

Letícia Sampaio ◽

Priscila Kobayashi ◽

Renee Laufer-Amorim ◽

João Carlos Ferreira ◽

...

Keyword(s):

Periodic Acid ◽

Prostate Gland ◽

Specific Antigen ◽

Species Conservation ◽

Ultrastructural Feature ◽

Secretory Cells ◽

Morphological Description ◽

Basal Cells ◽

Periodic Acid Schiff ◽

Young Subjects

The giant anteater (Myrmecophaga tridactyla) is a vulnerable species from Central and South America, and is considered possibly extinct in Belize, Guatemala, El Salvador, and Uruguay. Due to the species’ conservation and reproductive importance, this research aimed to characterize the morphology, histochemical, immunohistochemical, and ultrastructural feature of the giant anteater prostate gland. For this, we collected 11 giant anteater prostate glands and performed macroscopic, morphological, histochemical, immunohistochemical, and ultrastructural analysis. Nine prostate glands from an adult subject and two from young subjects were studied. Grossly, the adult giant anteater prostate gland is divided in two distinct zones; the central zones (composed mainly of ducts) and the peripheral zones (of acini formed by secretory cells). The secretory cells showed positive periodic acid–Schiff staining. Furthermore, the immunohistochemical characterization revealed a similar human prostate pattern, with p63 staining basal cells, uroplakin III (UPIII) superficial cells of prostatic urethra, androgen receptor (AR) expressing nucleus of secretory and stromal cells, and prostatic specific antigen (PSA) staining prostatic epithelial cells. Overall, our research provided an in-depth morphological description of the giant anteater’s prostate gland, providing valuable information for futures studies focused on giant anteater conservation.

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Nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate splitting enzyme(s) of sheep and rabbit erythrocytes: their effect on the growth of Haemophilus

Canadian Journal of Microbiology ◽

10.1139/m82-104 ◽

1982 ◽

Vol 28 (6) ◽

pp. 696-702 ◽

Cited By ~ 5

Author(s):

M. Artman ◽

G. Frankl

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Nicotinamide Adenine Dinucleotide ◽

Nicotinamide Adenine Dinucleotide Phosphate ◽

Nicotinamide Adenine ◽

Sheep Erythrocytes ◽

Sheep Blood ◽

Highly Active ◽

Inhibition Of Growth ◽

Gradual Release

Fragility of rabbit erythrocytes in agar plates results in gradual release of their NAD and NADP contents into the medium. Due to high NADase and negligible NADPase activity of rabbit red blood cell stroma at neutral pH, the NAD released into the medium is hydrolyzed and NADP remains intact. Thus, rabbit erythrocytes and their lysates support the growth of NAD(P)-requiring Haemophilus by serving as a source of NADP.Stability of sheep erythrocytes in agar plates results in retention of their NAD and NADP contents and consequently in inhibition of growth of NAD(P)-requiring Haemophilus. The highly active NAD- and NADP-splitting enzyme(s) of sheep red blood cell stroma prevent(s) the growth of Haemophilus on sheep blood lysates through inactivation of both NAD and NADP which are released into the medium.

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Structural and Ultrastructural Morphological Evaluation of Giant Anteater (Myrmecophaga Tridactyla) Prostate Gland

10.21203/rs.3.rs-195228/v1 ◽

2021 ◽

Author(s):

Fernanda B C de Moura ◽

Letícia H. T. S. Sampaio ◽

Priscila E. Kobayashi ◽

Renee Laufer-Amorim ◽

João Carlos P. Ferreira ◽

...

Keyword(s):

Stromal Cells ◽

Periodic Acid ◽

Prostate Gland ◽

Ultrastructural Feature ◽

Secretory Cells ◽

Morphological Description ◽

Basal Cells ◽

Periodic Acid Schiff ◽

Morphological Evaluation ◽

Young Subjects

Abstract The giant anteater (Myrmecophaga tridactyla) is a vulnerable species from Central and South Americas, that is considered possibly extinct in Belize, Guatemala, El Salvador, and Uruguay. Due to the species conservations and reproduction’s importance, this research aimed to characterize the morphology, histochemical, immunohistochemical, and ultrastructural feature of the giant anteater prostate gland. For this, we collected 11 giant anteater prostate glands and performed macroscopic, morphological, histochemical, immunohistochemical, and ultrastructural analysis. Nine-prostate glands from adult and two from young subjects were studied. Grossly, the adult giant anteater prostate gland is divided in two distinct zones; the central zones composed mainly of ducts and the peripheral zones of acini formed by secretory cells. The secretory cells showed positive periodic acid–Schiff staining. Furthermore, the immunohistochemical characterization revealed a similar human prostate pattern, with p63 staining basal cells, UPIII superficial cells of prostatic urethra, AR expressing nucleus of secretory and stromal cells, and PSA staining prostatic epithelial cells. Overall, our research provided an indepth morphological description of the giant anteater prostate gland, providing a valuable information for futures studies focused on giant anteater conservation.

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Effect of Niacin on Mitochondria of Allium Cepa Root Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060404 ◽

1967 ◽

Vol 25 ◽

pp. 78-79

Author(s):

M. Arif Hayat

Keyword(s):

Nicotinamide Adenine Dinucleotide ◽

Hydrogen Transfer ◽

Nicotinamide Adenine Dinucleotide Phosphate ◽

Nicotinamide Adenine ◽

Root Tips ◽

Root Cells ◽

Transfer Processes ◽

Standard Procedures ◽

A Cell ◽

Critical Interest

Although it is recognized that niacin (pyridine-3-carboxylic acid), incorporated as the amide in nicotinamide adenine dinucleotide (NAD) or in nicotinamide adenine dinucleotide phosphate (NADP), is a cofactor in hydrogen transfer in numerous enzyme reactions in all organisms studied, virtually no information is available on the effect of this vitamin on a cell at the submicroscopic level. Since mitochondria act as sites for many hydrogen transfer processes, the possible response of mitochondria to niacin treatment is, therefore, of critical interest.Onion bulbs were placed on vials filled with double distilled water in the dark at 25°C. After two days the bulbs and newly developed root system were transferred to vials containing 0.1% niacin. Root tips were collected at ¼, ½, 1, 2, 4, and 8 hr. intervals after treatment. The tissues were fixed in glutaraldehyde-OsO4 as well as in 2% KMnO4 according to standard procedures. In both cases, the tissues were dehydrated in an acetone series and embedded in Reynolds' lead citrate for 3-10 minutes.

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