DRY WEIGHT DETERMINATION OF SINGLE LYOPHILIZED MAST CELLS OF THE RAT

Dry weight and fat-free dry weight determinations are presented for single lyophilized rat peritoneal mast cells together with an average value for the protein content. By combining these results with values in the literature for heparin, histamine and serotonin, it is possible to account for most of the dry weight of rat mast cells.

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DETERMINATION OF l-HISTIDINE IN NANOGRAM AMOUNTS AND THE QUESTION OF ITS OCCURRENCE IN MAST CELLS

Journal of Histochemistry & Cytochemistry ◽

10.1177/20.11.917 ◽

1972 ◽

Vol 20 (11) ◽

pp. 917-922 ◽

Cited By ~ 4

Author(s):

DAVID I. WILKINSON ◽

DAVID GLICK

Keyword(s):

Mast Cells ◽

Rate Limiting Step ◽

Limiting Step ◽

Peritoneal Mast Cells ◽

Before And After ◽

Chromatographic Detection ◽

Rat Peritoneal Mast Cells ◽

Rate Limiting

In an attempt to clarify the question of whether histidine is stored in the mast cell for coversion to histamine or whether the rate of conversion is rapid enough to prevent accumulation of histidine so that the rate-limiting step is the histidine uptake, it was found that no histidine was demonstrable in rat peritoneal mast cells by either quantitative analysis or paper chromatographic detection. Microadaptation of Hassall's method, based on conversion of l-histidine by histidase to urocanic acid and measurement of the latter by its absorbance at 277 nm, was made to permit determination of histidine in nanogram amounts in the presence of histamine. This adaptation was found reliable when compared with the o-phthalaldehyde method in estimation of l-histidine in serum and in insulin hydrolysate, and then it was applied to analysis of mast cells before and after l-histidine uptake in vitro. The adaptation should be generally useful in microanalysis of l-histidine in histologically and cytologically defined samples.

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Immunocytochemical identification of immature rat peritoneal mast cells using a monoclonal antibody specific for rat mast cells

Acta Histochemica ◽

10.1016/s0065-1281(97)80004-9 ◽

1997 ◽

Vol 99 (1) ◽

pp. 23-27 ◽

Cited By ~ 3

Author(s):

Cloris D. Faraco ◽

Itamar Vugman ◽

Reuben P. Siraganian ◽

Maria Celia Jamur ◽

Constance Oliver

Keyword(s):

Monoclonal Antibody ◽

Mast Cells ◽

Immature Rat ◽

Peritoneal Mast Cells ◽

Rat Peritoneal Mast Cells ◽

Rat Mast Cells

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Surface TLR2 and TLR4 Expression on Mature Rat Mast Cells Can Be Affected by Some Bacterial Components and Proinflammatory Cytokines

Mediators of Inflammation ◽

10.1155/2011/427473 ◽

2011 ◽

Vol 2011 ◽

pp. 1-11 ◽

Cited By ~ 21

Author(s):

Anna Pietrzak ◽

Maciej Wierzbicki ◽

Magdalena Wiktorska ◽

Ewa Brzezińska-Błaszczyk

Keyword(s):

Flow Cytometry ◽

Mast Cell ◽

Mast Cells ◽

Proinflammatory Cytokines ◽

Tlr4 Expression ◽

Cysteinyl Leukotriene ◽

Peritoneal Mast Cells ◽

Rat Peritoneal Mast Cells ◽

Activation Conditions ◽

Rat Mast Cells

The aim of our study was to determine whether some bacterial components as well as some proinflammatory cytokines can affect surface mast cell levels. By the use of flow cytometry technique, we documented that freshly isolated mature rat peritoneal mast cells do express surface TLR2 and TLR4 protein, but not CD14 molecules, and respond to stimulation with TLR2 and TLR4 ligands by cysteinyl leukotriene generation. The level of TLR2 protein is modulated by PGN and CCL5 treatment, but not by LPS, LAM, TNF, or IL-6. Surface mast cell TLR4 expression is affected by LPS, LAM, IL-6, and CCL5. Considering that TLR-mediated activation conditions not only engaged these cells in antibacterial defense and development of inflammation but also might influence allergic processes, our observations that surface TLR2 and TLR4 expression can be regulated both bacterial components and proinflammatory cytokines seem to be very intriguing and importance.

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Effects of a Moderately Lower Temperature on the Proliferation and Degranulation of Rat Mast Cells

Journal of Immunology Research ◽

10.1155/2016/8439594 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Ruoyu Wang ◽

Xiaoqin Yin ◽

Hui Zhang ◽

Jiwei Wang ◽

Lin Chen ◽

...

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Allergic Diseases ◽

Effector Cells ◽

Peritoneal Mast Cells ◽

Ige Mediated ◽

Rat Peritoneal Mast Cells ◽

Mast Cell Line ◽

Lower Temperature ◽

Rat Mast Cells

Mast cells are traditionally considered as key effector cells in IgE-mediated allergic diseases. However, the roles of mast cells have also been implicated in diverse physiological and pathological processes. Mast cells are distributed in various organs and tissues of various species. Some of the organs and tissues, such as testis, skin, and the upper part of the respiratory tract, have a temperature that is lower than the body’s core temperature. The purpose of the present study was to investigate the effects of a lower temperature on the proliferation and degranulation of rat mast cells. Here, we demonstrate that cell growth was retarded at 35°C compared to 37°C for both rat peritoneal mast cells (RPMC) and RBL-2H3, a rat mast cell line. Furthermore, RPMC became more susceptible to degranulation at 35°C compared to 37°C. In contrast, degranulation of RBL-2H3 was not as sensitive to temperature change as RPMC. The functionality of mast cells in unique organs with a lower temperature warrants further analysis.

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Latexin, a carboxypeptidase A inhibitor, is expressed in rat peritoneal mast cells and is associated with granular structures distinct from secretory granules and lysosomes

Biochemical Journal ◽

10.1042/bj3460817 ◽

2000 ◽

Vol 346 (3) ◽

pp. 817-826 ◽

Cited By ~ 11

Author(s):

Yoshihiko URATANI ◽

Keiko TAKIGUCHI-HAYASHI ◽

Nobuhiko MIYASAKA ◽

Michio SATO ◽

Ming-hao JIN ◽

...

Keyword(s):

Mast Cells ◽

Secretory Granules ◽

Minority Population ◽

Carboxypeptidase A ◽

Unique Type ◽

Peritoneal Mast Cells ◽

Neural Tissues ◽

Rat Peritoneal Mast Cells ◽

Silica Gel Particles ◽

Rat Mast Cells

Latexin, a protein possessing inhibitory activity against rat carboxypeptidase A1 (CPA1) and CPA2, is expressed in a neuronal subset in the cerebral cortex and cells in other neural and non-neural tissues of rat. Although latexin also inhibits mast-cell CPA (MCCPA), the expression of latexin in rat mast cells has not previously been confirmed. In the present study we examined the expression and subcellular localization of latexin in rat peritoneal mast cells. Western blot and reverse-transcriptase-mediated PCR analyses showed that latexin was contained and expressed in the rat peritoneal mast cells. Immunocytochemically, latexin immunofluorescence was localized on granular structures distinct from MCCPA-, histamine- or cathepsin D-immunopositive granules. Immunoelectron microscopy revealed that latexin was associated with a minority population of granules. The latexin-associated granules were separated from MCCPA- or histamine-containing granules on a self-generating density gradient of polyvinylpyrrolidone-coated silica-gel particles (Percoll). Treatments with high ionic strength and heparinase released latexin from the granules, suggesting that latexin is non-covalently associated with a heparin-like component of the granules. MCCPA and histamine were released from the mast cells after non-immunological and immunological stimulation with compound 48/80, A23187 and anti-IgE antibody, whereas latexin was not released. These results show that latexin is synthesized in rat peritoneal mast cells and suggest that it is associated with a unique type of intracellular granules distinct from MCCPA- and histamine-containing secretory granules and lysosomes.

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