Direct detection of morphine in human urine by surface-ionization mass spectrometry

Detection and analysis of drugs-of-abuse in biological fluids are the essential tasks for the law enforcement, forensic toxicology, doping research and social health. In this work, we developed a surface-ionization mass spectrometry method for direct detection of trace amount of morphine spiked in blank urine. The mass-spectrometric analysis of spiked samples was carried out without preliminary extraction and chromatographic separation. It was found out that the major fragment ions at m/z 144, 146 could be served as indicator lines of morphine spiked in blank urine. Limit of detection of morphine spiked in blank urine was 100 pg (50 ng/ml), and a linear range of calibration curve was more than two orders of magnitude. The spiked samples were also analyzed by gas chromatography–mass spectrometry without preliminary extraction and derivatization procedures. No morphine was found in the spiked samples. The experimental data show that the high selectivity of the surface ionization can be allowed to direct analysis of morphine spiked in blank urine without its preliminary extraction and chromatographic separation.

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Development of surface ionization mass spectrometry for detection of stimulants in human urine

European Journal of Mass Spectrometry ◽

10.1177/14690667211002777 ◽

2021 ◽

pp. 146906672110027

Author(s):

Shovkatjon Akhunov ◽

Khatam Ashurov ◽

Sherzod Axmedov ◽

Beknazar Kasimov ◽

Vladimir Rotshteyn ◽

...

Keyword(s):

Mass Spectrometry ◽

Real World ◽

Chromatographic Separation ◽

Limit Of Detection ◽

Direct Detection ◽

Extraction Procedure ◽

Mass Spectrometry Data ◽

Ionization Mass Spectrometry ◽

Surface Ionization ◽

Ionization Mass

This paper demonstrates direct detection of stimulants such as amphetamine, methamphetamine and cocaine spiked with untreated urine and a real world sample using surface ionization mass spectrometry. Spiked samples were analyzed without preliminary chromatographic separation and extraction procedure using the developed method. Moreover, in order to check the analytical capabilities of the method non-extracted real world sample was analyzed. After liquid–liquid extraction, the same sample was analyzed using the method for comparative study. Limit of detection of spiked samples was in the range of 10 pg (10 ng/ml) to 100 pg (100 ng/ml). Linear ranges of samples were two orders of magnitude or more than two orders of magnitude. It was revealed these spiked samples and real world sample can be analyzed without preliminary chromatographic separation and preliminary extraction procedure due to high selectivity of the method and the presence of the indicator lines of studied analytes in the mass spectra. The surface ionization mass spectrometry data was attested by the GC/MS analysis of these samples.

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Direct detection of illicit drugs from biological fluids by desorption/ionization mass spectrometry with nanoporous silicon microparticles

The Analyst ◽

10.1039/c5an01754h ◽

2015 ◽

Vol 140 (23) ◽

pp. 7926-7933 ◽

Cited By ~ 14

Author(s):

T. M. Guinan ◽

P. Kirkbride ◽

C. B. Della Vedova ◽

S. G. Kershaw ◽

H. Kobus ◽

...

Keyword(s):

Mass Spectrometry ◽

Illicit Drugs ◽

Direct Detection ◽

Biological Fluids ◽

Laser Desorption ◽

Ionization Mass Spectrometry ◽

Ionization Mass ◽

Laser Desorption Ionization ◽

Nanoporous Silicon ◽

Desorption Ionization

Surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) with porous silicon microparticles was used for the all-in-one extraction and detection of illicit drugs from saliva, urine and plasma.

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