KLF2 Inhibits the Migration and Invasion of Prostate Cancer Cells by Downregulating MMP2

KLF2, a member of the Kruppel-like factor (KLF) family, is thought to be a tumor suppressor in many kinds of malignant tumors. Its functions in prostate cancer (PCa) are unknown. This study aimed to explore the role of KLF2 in the migration and invasion of PCa cells. The expression of KLF2 was measured by immunohistochemistry in PCa tissues and in paired non-tumor tissues. KLF2 and MMP2 expression in cells was measured by Western blot and RT-qPCR. Adenoviruses and siRNAs were used in cell function tests to investigate the role of KLF2 in regulating MMP2. Interactions between KLF2 and MMP2 were analyzed by a luciferase activity assay. The present study, for the first time, identified that KLF2 was downregulated both in PCa clinical tissue samples and in cancer cell lines. The overexpression of KLF2 inhibited the migration and invasion of PCa cells via the suppression of MMP2.This study demonstrates that KLF2 might act as a tumor suppressor gene in PCa and that the pharmaceutical upregulation of KLF2 may be a potential approach for treatment.

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The Role of hCDC4 as a Tumor Suppressor Gene in Genomic Instability Underlying Prostate Cancer

10.21236/ada463404 ◽

2006 ◽

Author(s):

Audrey Van Drogen ◽

Charles H. Spruck

Keyword(s):

Prostate Cancer ◽

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Genomic Instability ◽

Suppressor Gene

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685. The Role of PTEN Tumor Suppressor Gene in Prostate Cancer Derived by Prostate Specific Deletion of PTEN

Molecular Therapy ◽

10.1016/s1525-0016(16)44891-4 ◽

2007 ◽

Vol 15 ◽

pp. S263

Keyword(s):

Prostate Cancer ◽

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Suppressor Gene ◽

Specific Deletion

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The role of tumor suppressor gene SOX11 in prostate cancer

Tumor Biology ◽

10.1007/s13277-015-3296-3 ◽

2015 ◽

Vol 36 (8) ◽

pp. 6133-6138 ◽

Cited By ~ 15

Author(s):

Zhiyong Yao ◽

Bin Sun ◽

Quan Hong ◽

Jingmin Yan ◽

Dawei Mu ◽

...

Keyword(s):

Prostate Cancer ◽

Tumor Suppressor ◽

Tumor Suppressor Gene ◽

Suppressor Gene

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YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Genes & Genomics ◽

10.1007/s13258-021-01175-z ◽

2021 ◽

Author(s):

Weijian Li ◽

Gaohuang Chen ◽

Zhenyu Feng ◽

Baoyi Zhu ◽

Lilin Zhou ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cell Function ◽

Migration And Invasion ◽

Sequencing Analysis ◽

Prostate Cancer Cells ◽

Mrna Sequencing ◽

Qrt Pcr ◽

Patient Prognosis

Abstract Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Differentially expressed mRNAs were identified by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verified that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

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