scholarly journals Serotype O:8 isolates in theYersinia pseudotuberculosiscomplex have different O-antigen gene clusters and produce various forms of rough LPS

2016 ◽  
Vol 22 (3) ◽  
pp. 205-217 ◽  
Author(s):  
Johanna J Kenyon ◽  
Katarzyna A Duda ◽  
Antonia De Felice ◽  
Monica M Cunneen ◽  
Antonio Molinaro ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Antigen Gene ◽  
O Antigen ◽  
Serotype O

scholarly journals Structure and genetics of Escherichia coli O antigens

2019 ◽  
Vol 44 (6) ◽  
pp. 655-683 ◽  
Author(s):  
Bin Liu ◽  
Axel Furevi ◽  
Andrei V Perepelov ◽  
Xi Guo ◽  
Hengchun Cao ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Basis ◽  
Structural Diversity ◽  
Gene Clusters ◽  
Current Standard ◽  
E Coli ◽  
Antigen Gene ◽  
O Antigen ◽  
Or Groups ◽  
O Antigens

ABSTRACT Escherichia coli includes clonal groups of both commensal and pathogenic strains, with some of the latter causing serious infectious diseases. O antigen variation is current standard in defining strains for taxonomy and epidemiology, providing the basis for many serotyping schemes for Gram-negative bacteria. This review covers the diversity in E. coli O antigen structures and gene clusters, and the genetic basis for the structural diversity. Of the 187 formally defined O antigens, six (O31, O47, O67, O72, O94 and O122) have since been removed and three (O34, O89 and O144) strains do not produce any O antigen. Therefore, structures are presented for 176 of the 181 E. coli O antigens, some of which include subgroups. Most (93%) of these O antigens are synthesized via the Wzx/Wzy pathway, 11 via the ABC transporter pathway, with O20, O57 and O60 still uncharacterized due to failure to find their O antigen gene clusters. Biosynthetic pathways are given for 38 of the 49 sugars found in E. coli O antigens, and several pairs or groups of the E. coli antigens that have related structures show close relationships of the O antigen gene clusters within clades, thereby highlighting the genetic basis of the evolution of diversity.

scholarly journals Molecular and Genetic Analyses of the Putative Proteus O Antigen Gene Locus

2010 ◽  
Vol 76 (16) ◽  
pp. 5471-5478 ◽  
Author(s):  
Quan Wang ◽  
Agnieszka Torzewska ◽  
Xiaojuan Ruan ◽  
Xiaoting Wang ◽  
Antoni Rozalski ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Gene Clusters ◽  
Molecular Classification ◽  
Opportunistic Pathogens ◽  
Antigen Gene ◽  
O Antigen ◽  
Tract Infections ◽  
High Degree

ABSTRACT Proteus species are well-characterized opportunistic pathogens primarily associated with urinary tract infections (UTI) of humans. The Proteus O antigen is one of the most variable constituents of the cell surface, and O antigen heterogeneity is used for serological classification of Proteus isolates. Even though most Proteus O antigen structures have been identified, the O antigen locus has not been well characterized. In this study, we identified the putative Proteus O antigen locus and demonstrated this region's high degree of heterogeneity by comparing sequences of 40 Proteus isolates using PCR-restriction fragment length polymorphism (RFLP). This analysis identified five putative Proteus O antigen gene clusters, and the probable functions of these O antigen-related genes were proposed, based on their similarity to genes in the available databases. Finally, Proteus-specific genes from these five serogroups were identified by screening 79 strains belonging to the 68 Proteus O antigen serogroups. To our knowledge, this is the first molecular characterization of the putative Proteus O antigen locus, and we describe a novel molecular classification method for the identification of different Proteus serogroups.

scholarly journals Genetic Analysis of the Cronobacter sakazakii O4 to O7 O-Antigen Gene Clusters and Development of a PCR Assay for Identification of All C. sakazakii O Serotypes

2012 ◽  
Vol 78 (11) ◽  
pp. 3966-3974 ◽  
Author(s):  
Yamin Sun ◽  
Min Wang ◽  
Quan Wang ◽  
Boyang Cao ◽  
Xin He ◽  
...  
Keyword(s):  
Gene Clusters ◽  
Cronobacter Sakazakii ◽  
Pcr Assay ◽  
Gram Negative ◽  
Content Type ◽  
Antigen Gene ◽  
O Antigen ◽  
Multiplex Pcr Assay ◽  
Food Borne ◽  
Invasive Infections

ABSTRACTThe Gram-negative bacteriumCronobacter sakazakiiis an emerging food-borne pathogen that causes severe invasive infections in neonates. Variation in the O-antigen lipopolysaccharide in the outer membrane provides the basis for Gram-negative bacteria serotyping. The O-antigen serotyping scheme forC. sakazakii, which includes seven serotypes (O1 to O7), has been recently established, and the O-antigen gene clusters and specific primers for threeC. sakazakiiserotypes (O1, O2, and O3) have been characterized. In this study, theC. sakazakiiO4, O5, O6, and O7 O-antigen gene clusters were sequenced, and gene functions were predicted on the basis of homology.C. sakazakiiO4 shared a similar O-antigen gene cluster withEscherichia coliO103. The general features and anomalies of all sevenC. sakazakiiO-antigen gene clusters were evaluated and the relationship between O-antigen structures and their gene clusters were investigated. Serotype-specific genes for O4 to O7 were identified, and a molecular serotyping method for allC. sakazakiiO serotypes, a multiplex PCR assay, was developed by screening against 136 strains ofC. sakazakiiand closely related species. The sensitivity of PCR-based serotyping method was determined to be 0.01 ng of genomic DNA and 103CFU of each strain/ml. This study completes the elucidation ofC. sakazakiiO-antigen genetics and provides a molecular method suitable for the identification ofC. sakazakiiO1 to O7 strains.

scholarly journals The Escherichia coli Serogroup O1 and O2 Lipopolysaccharides Are Encoded by Multiple O-antigen Gene Clusters

2017 ◽  
Vol 7 ◽  
Author(s):  
Sabine Delannoy ◽  
Lothar Beutin ◽  
Patricia Mariani-Kurkdjian ◽  
Aubin Fleiss ◽  
Stéphane Bonacorsi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Clusters ◽  
Antigen Gene ◽  
O Antigen

scholarly journals The O-Antigen Gene Cluster of Escherichia coli O55:H7 and Identification of a New UDP-GlcNAc C4 Epimerase Gene

2002 ◽  
Vol 184 (10) ◽  
pp. 2620-2625 ◽  
Author(s):  
Lei Wang ◽  
Sandy Huskic ◽  
Adam Cisterne ◽  
Deborah Rothemund ◽  
Peter R. Reeves
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Gene Cluster ◽  
Gene Clusters ◽  
The Other ◽  
Recombination Site ◽  
E Coli ◽  
Antigen Gene ◽  
O Antigen ◽  
Usual Location

ABSTRACT Escherichia coli O55 is an important antigen which is often associated with enteropathogenic E. coli clones. We sequenced the genes responsible for its synthesis and identified genes for O-antigen polymerase, O-antigen flippase, four enzymes involved in GDP-colitose synthesis, and three glycosyltransferases, all by comparison with known genes. Upstream of the normal O-antigen region there is a gne gene, which encodes a UDP-GlcNAc epimerase for converting UDP-GlcNAc to UDP-GalNAc and is essential for O55 antigen synthesis. The O55 gne product has only 20 and 26% identity to the gne genes of Pseudomonas aeruginosa and E. coli O113, respectively. We also found evidence for the O55 gene cluster's having evolved from another gene cluster by gain and loss of genes. Only three of the GDP-colitose pathway genes are in the usual location, the other two being separated, although nearby. It is thought that the E. coli O157:H7 clone evolved from the O55:H7 clone in part by transfer of the O157 gene cluster into an O55 lineage. Comparison of genes flanking the O-antigen gene clusters of the O55:H7 and O157:H7 clones revealed one recombination site within the galF gene and located the other between the hisG and amn genes. Genes outside the recombination sites are 99.6 to 100% identical in the two clones, while most genes thought to have transferred with the O157 gene cluster are 95 to 98% identical.

scholarly journals Correction: Comparison of O-Antigen Gene Clusters of All O-Serogroups of Escherichia coli and Proposal for Adopting a New Nomenclature for O-Typing

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0154551 ◽  
Author(s):  
Chitrita DebRoy ◽  
Pina M. Fratamico ◽  
Xianghe Yan ◽  
GianMarco Baranzoni ◽  
Yanhong Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Clusters ◽  
Antigen Gene ◽  
O Antigen
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