scholarly journals Resveratrol Production from Hairy Root Cultures of Scutellaria baicalensis

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800
Author(s):  
Sang-Won Lee ◽  
Young Seon Kim ◽  
Md. Romij Uddin ◽  
Do Yeon Kwon ◽  
Yeon Bok Kim ◽  
...  
Keyword(s):  
Hairy Root ◽  
Scutellaria Baicalensis ◽  
Growth Media ◽  
Hairy Root Cultures ◽  
Ms Medium ◽  
Root Cultures ◽  
Full Strength ◽  
Alternative Approach ◽  
Half Strength ◽  
The Media

The levels of resveratrol produced by hairy root cultures of Scutellaria baicalensis were investigated using different media of varying strengths and in the presence of various concentrations of auxins. The levels of resveratrol were higher when the hairy root cultures were maintained in full-strength Murashige and Skoog (MS) medium when compared with other growth media. The cultures grown in full-strength MS medium produced 2.5-fold higher resveratrol than those grown in half-strength B5 medium—the lowest resveratrol-producing medium. The levels of resveratrol varied significantly when cultures were grown in full-strength MS with varying concentrations of auxins. Supplementation of the media with the auxin indole acetic acid (IAA) at 0.1 mg/L produced the highest accumulation of resveratrol. Our findings reveal a valuable alternative approach for the production of resveratrol from S. baicalensis.

scholarly journals Glucosinolate Biosynthesis in Hairy Root Cultures of Broccoli (Brassica oleracea var. italica)

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800
Author(s):  
Sun-Ju Kim ◽  
Woo Tae Park ◽  
Md. Romij Uddin ◽  
Yeon Bok Kim ◽  
Sang-Yong Nam ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Hairy Root ◽  
Glucosinolate Content ◽  
Growth Media ◽  
Hairy Root Cultures ◽  
Ms Medium ◽  
Root Cultures ◽  
Valuable Alternative ◽  
Alternative Approach ◽  
Very High

Here we present previously unreported glucosinolate production by hairy root cultures of broccoli (B. oleracea var. italica). Growth media greatly influenced the growth and glucosinolate content of hairy root cultures of broccoli. Seven glucosinolates, glucoraphanin, gluconapin, glucoerucin, glucobrassicin, 4- methoxyglucobrassicin, gluconasturtiin, and neoglucobrassicin, were identified by analysis of the broccoli hairy root cultures. Both half and full strength B5 and SH media enabled the highest accumulation of glucosinolates. In most cases, the levels of glucosinolates were higher in SH and BS media. Among the 7 glucosinolates, the accumulation of neoglucobrassicin was very high, irrespective of growth medium. The neoglucobrassicin content was 7.4-fold higher in SH medium than 1/2 MS, in which its level was the lowest. The 1/2 B5 medium supported the production of the highest amounts of glucobrassicin and 4- methoxyglucobrassicin, the levels for which were 36.2- and 7.9- fold higher, respectively, than their lowest content in 1/2 MS medium. The 1/2 SH medium enabled the highest accumulation of glucoraphanin and gluconapin in the broccoli hairy root cultures, whose levels were 1.8- and 4.6-fold higher, respectively, than their lowest content in 1/2 MS medium. Our results suggest that hairy root cultures of broccoli could be a valuable alternative approach for the production of glucosinolate compounds.

scholarly journals Influence of Different Carbohydrates on Flavonoid Accumulation in Hairy Root Cultures of Scutellaria baicalensis

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Chang Ha Park ◽  
Young Seon Kim ◽  
Xiaohua Li ◽  
Haeng Hoon Kim ◽  
Mariadhas Valan Arasu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Hairy Root ◽  
Carbon Sources ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Scutellaria Baicalensis ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Carbohydrate Source ◽  
Half Strength

Carbohydrate sources play important roles in energy and growth of plants. Therefore, in this study, we investigated the optimal carbohydrate source in hairy root cultures (HRCs) of Scutellaria baicalensis infected with Agrobacterium rhizogenes strain R1000. The hairy roots were cultured in half-strength B5 liquid medium supplemented with seven different carbohydrates sources (sucrose, fructose, glucose, galactose, sorbitol, mannitol and maltose), each at a concentration of 100 mM, in order to identify the best carbon sources for the production of major flavones, such as wogonin, baicalin and baicalein. Sucrose, galactose and fructose markedly influenced the production of major flavones and were therefore chosen for subsequent experiments. HRC growth and flavone accumulation were examined following culture with 30, 100 and 150 mM sucrose, galactose and fructose, respectively. From these data, 150 mM sucrose was found to be the optimal carbon source for the enhancement of baicalein production and growth of S. baicalensis HRCs. Fructose caused the greatest increase in baicalin accumulation. Additionally, galactose was the optimal carbon source for wogonin production. These results provide important insights into the optimal growth conditions, particularly the appropriate carbohydrate source, for S. baicalensis.

Flavonoids and phenylethanoids from hairy root cultures of Scutellaria baicalensis

1997 ◽  
Vol 44 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Yu Zhou ◽  
Masao Hirotani ◽  
Takafumi Yoshikawa ◽  
Tsutomu Furuya
Keyword(s):  
Hairy Root ◽  
Scutellaria Baicalensis ◽  
Hairy Root Cultures ◽  
Root Cultures

Cloning and expression of UDP-glucose: flavonoid 7-O-glucosyltransferase from hairy root cultures of Scutellaria baicalensis

Planta ◽  
2000 ◽  
Vol 210 (6) ◽  
pp. 1006-1013 ◽  
Author(s):  
Masao Hirotani ◽  
Ryo Kuroda ◽  
Hideyuki Suzuki ◽  
Takafumi Yoshikawa
Keyword(s):  
Hairy Root ◽  
Scutellaria Baicalensis ◽  
Cloning And Expression ◽  
Hairy Root Cultures ◽  
Root Cultures

scholarly journals Production of Bacalin, Bacalein and Wogonin in Hairy Root Culture of American Skullcap (Scutellaria lateriflora)by Auxin Treatment

2017 ◽  
Vol 14 (2) ◽  
pp. 673-677 ◽  
Author(s):  
Do Yeon Kwon ◽  
Haeng Hoon Kim ◽  
Jong Seok Park ◽  
Sang Un Park ◽  
Nam Il Park
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Control Treatment ◽  
Naphthaleneacetic Acid ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alternative Approach ◽  
Scutellaria Lateriflora

ABSTRACT: The hairy root culture of American Skullcap (Scutellaria lateriflora) was studied to investigate the biomass and flavonoids content (baicalin, baicalein and wogonin) in response of various auxin concentrations.The growth rates of the hairy roots varied significantly only at IBA 0.1 mg/L and for all other auxin treatments did not vary significantly. The biomass of hairy roots was 8% higher when treated with IBA 0.1 mg/L and biomass was almost similar and slightly lower levels when treated with various IAA concentration and NAA, respectively. However, the auxins treatments responsed positively to increase flavone production in American Skullcaphairy root culture. The auxin indole-3-butyric acid (IBA) at 1 mg/L performed the best for the accumulation of baicalin and wogonin. The auxin IBA at 1 mg/L accumulated 1.64 and 2.92 times higher baicalin and wogonin, respectively compared to control treatment. Meanwhile, the highest levels of baicalein were observed for hair root cultures in the presence of 1-naphthaleneacetic acid (NAA) at 0.1 mg/L achieving 2.38 times higher than that of accumulated in the control. These findings indicate that hairy root cultures of S. lateriflorausing liquid 1/2MS medium supplemented with auxin could be a valuable alternative approach for flavonoid production.

scholarly journals Production of hairy root cultures of lettuce (Lactuca sativa L.)

2014 ◽  
Vol 9 (12) ◽  
pp. 1196-1205 ◽  
Author(s):  
Tadić Vojin ◽  
Milošević Snežana ◽  
Cingel Aleksandar ◽  
Petrić Marija ◽  
Trifunović Milana ◽  
...  
Keyword(s):  
Hairy Root ◽  
Lactuca Sativa ◽  
Target Genes ◽  
Pcr Analysis ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Lactuca Sativa L ◽  
Half Strength ◽  
Independent Transformation

AbstractHairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

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