Studies on the basic issues relevant to sperm cryopreservation in humans

Rapid freezing and vitrification are becoming popular for sperm freezing in humans; however, basic and critical issues relevant to sperm cryopreservation remain to be resolved. The aims of the present study were to study the effects of osmolality of freezing medium, sperm concentrations, thawing methods, and sugars (sucrose and trehalose) on sperm motility and DNA integrity by rapid freezing using 0.5 ml standard straws loaded with 100 µl sperm each. The results showed that (1) the post-thaw recovery rates of total motility and progressive motility of sperm cryopreserved in freezing medium containing 0.25 M sucrose with 442 mOsm/kg osmolality were significantly higher ( p < 0.05) than that of sperm cryopreserved in freezing medium containing 0.25 M sucrose with 536 mOsm/kg osmolality (36.5 ± 2.8% and 36.9 ± 1.7% versus 30.4 ± 1.9% and 30.3 ± 2.9%, respectively), (2) cryopreservation of both total and progressive motilities was not significantly affected ( p > 0.05) by sperm concentrations in the range from 5 to 20 × 106 sperm/ml, (3) thawing method 37°C for 2 min was better than 42°C for 15 s in terms of post-thaw recovery rates of both total and progressive motilities ( p < 0.05), (4) 0.25 M trehalose was better than 0.25 M sucrose in cryopreserving both total and progressive motilities ( p < 0.05), and (5) sperm nuclear DNA is relatively resistant to the changes of the above factors compared with sperm motility. It was concluded that human sperm can be best cryopreserved by rapid freezing using 0.25 M sucrose or trehalose with osmolality 442 to 457 mOsm/kg at high sperm concentration followed by thawing at 37°C. Trehalose is a stronger cryoprotectant than sucrose for sperm cryopreservation.

Download Full-text

Comparison of human sperm quality and nuclear DNA integrity between slow and rapid freezing

Journal of Women s Medicine ◽

10.5468/jwm.2010.3.2.57 ◽

2010 ◽

Vol 3 (2) ◽

pp. 57

Author(s):

Byung Chul Jee ◽

Hye Jin Chang ◽

Yong Tark Jeon ◽

Jung Ryeol Lee ◽

Chang Suk Suh ◽

...

Keyword(s):

Nuclear Dna ◽

Sperm Quality ◽

Human Sperm ◽

Dna Integrity ◽

Rapid Freezing

Download Full-text

Cytotoxic Effects of Benzene Metabolites on Human Sperm Function: An In Vitro Study

ISRN Toxicology ◽

10.1155/2013/397524 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Priyanka Mandani ◽

Ketki Desai ◽

Hyacinth Highland

Keyword(s):

Sperm Motility ◽

Nuclear Dna ◽

In Vitro Study ◽

Human Sperm ◽

Dna Integrity ◽

Sperm Function ◽

Cytotoxic Effects ◽

Membrane Function ◽

Atp Production

In recent years, individuals are rampantly exposed to vapours of benzene, through paint, plastic, petroleum industries, fuel exhaust, and tobacco smoke. Hence the present investigation was directed towards determining the effect of benzene metabolites, namely, phenol-hydroquinone and catechol, on the motility, viability, and nuclear integrity of the human spermatozoa. From the results obtained it was clear that exposure to phenol-hydroquinone caused a significant decline in both, sperm motility and viability. Exposure to a phenol-hydroquinone (Phase I) microenvironment may therefore inhibit metabolically active enzymes, thus impeding ATP production, and in turn lowers sperm motility and viability. In addition, the present study also revealed that both metabolites of benzene caused significant denaturation of sperm nuclear DNA. Hence, exposure to phenol-hydroquinone in vitro could have resulted in generation of free radicals and altered membrane function, which is reflected by a decline in the motility, viability, and loss of sperm nuclear DNA integrity. In Phase II, the exposure of human sperm in vitro to varied concentrations of catechol caused only insignificant changes in sperm motility and viability as compared to those observed on exposure to phenol-hydroquinone. Hence, exposure to catechol appeared to have less toxic effects than those of phenol-hydroquinone.

Download Full-text

Human sperm cryopreservation: the effects of slow freeze versus snap freeze protocols on sperm motility

Fertility and Sterility ◽

10.1016/j.fertnstert.2009.07.1424 ◽

2009 ◽

Vol 92 (3) ◽

pp. S196

Author(s):

B.T. Kloos ◽

A. Finn ◽

D. Davies ◽

O. Ocali ◽

J. Hill ◽

...

Keyword(s):

Sperm Motility ◽

Human Sperm ◽

Sperm Cryopreservation

Download Full-text

Electromagnetic field in human sperm cryopreservation improves fertilizing potential of thawed sperm through physicochemical modification of water molecules in freezing medium

PLoS ONE ◽

10.1371/journal.pone.0221976 ◽

2019 ◽

Vol 14 (9) ◽

pp. e0221976 ◽

Cited By ~ 2

Author(s):

Dariush Gholami ◽

Seyed Mahmood Ghaffari ◽

Gholamhossein Riazi ◽

Rouhollah Fathi ◽

James Benson ◽

...

Keyword(s):

Electromagnetic Field ◽

Human Sperm ◽

Water Molecules ◽

Sperm Cryopreservation ◽

Freezing Medium

Download Full-text

The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

Reproduction Fertility and Development ◽

10.1071/rd15041 ◽

2016 ◽

Vol 28 (10) ◽

pp. 1518 ◽

Cited By ~ 4

Author(s):

Urszula Marzec-Wróblewska ◽

Piotr Kamiński ◽

Paweł Łakota ◽

Marek Szymański ◽

Karolina Wasilow ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Morphology ◽

Sperm Quality ◽

Sperm Concentration ◽

Human Sperm ◽

Human Semen ◽

Glutathione Peroxidase Activity ◽

Normal Sperm ◽

Group I ◽

Gpx Activity

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

Download Full-text

Raw Semen Characteristics of Three Different Indonesian Local Roosters

ANIMAL PRODUCTION ◽

10.20884/1.anprod.2016.18.3.555 ◽

2016 ◽

Vol 18 (3) ◽

pp. 165

Author(s):

Emilia Kamung Hambu ◽

Raden Iis Arifiantini ◽

Bambang Purwantara ◽

Sri Darwati

Keyword(s):

Sperm Motility ◽

Semen Quality ◽

Mass Movement ◽

Sperm Concentration ◽

Semen Volume ◽

Sperm Abnormality ◽

Microscopic Evaluation ◽

Semen Characteristics ◽

Live Sperm ◽

Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of Merawang roosters, Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of Merawang (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters. Sperm concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

Download Full-text

Evaluation of the Efficiency of Two Different Freezing Media and Two Different Protocols to Preserve Human Spermatozoa from Cryoinjury

International Journal of Reproductive Medicine ◽

10.1155/2016/6059757 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Gemma Fabozzi ◽

Maria Flavia Starita ◽

Emilia Rega ◽

Alessandra Alteri ◽

Antonio Colicchia ◽

...

Keyword(s):

Sperm Quality ◽

Human Sperm ◽

Human Spermatozoa ◽

Sperm Cryopreservation ◽

Progressive Motility ◽

Direct Addition ◽

Sperm Sample ◽

Sperm Survival ◽

Better Than

It is universally recognized that cryopreservation impairs sperm quality. In order to improve postthawing sperm survival and motility, media of different composition and different protocols have been proposed. However, no clear evidence is available to understand which are the most efficient protocol and medium for sperm cryopreservation. The present study evaluates the efficiency of two different cryopreservation protocols and two common freezing media (FM) containing different cryoprotectants (CPs), TEST Yolk Buffer (TYB) and Sperm Freeze (SF), to preserve human sperm quality. Our data suggest that TYB is better than SF both in terms of postthaw viability and in terms of progressive motility, while the direct addition of FM to the sperm sample resulted in the most efficient protocol in terms of postthaw viability but not in terms of progressive motility.

Download Full-text

Omeprazole does not alter human sperm motility, viability or DNA integrity in vitro

Andrologia ◽

10.1111/and.13260 ◽

2019 ◽

Vol 51 (6) ◽

pp. e13260 ◽

Cited By ~ 1

Author(s):

Saleem Ali Banihani ◽

Alayna'‐almarddyah Abdullah Al‐khawalde

Keyword(s):

Sperm Motility ◽

Human Sperm ◽

Dna Integrity

Download Full-text

Impacts administration of Rifampicin on sperm DNA integrity and Male Reproductive System parameters in rats

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00851 ◽

2021 ◽

pp. 4897-4902

Author(s):

Furqan Mohammed Al-Asady ◽

Dalia Abdulzahra Al-Saray

Keyword(s):

Sperm Motility ◽

Sperm Concentration ◽

Sperm Chromatin ◽

Dna Integrity ◽

Sperm Function ◽

High Dose ◽

Adult Rats ◽

Male Adult ◽

Sperm Dna Integrity ◽

Sperm Dna

Objective: Evaluate the impacts of rifampicin on certain sperm function parameters and to determine whether rifampicin has an impact on chromatin quality or sperm DNA integrity. Materials and Methods: Forty two male adult rats were subjected to this study. The entire rats were subjected to random division into six groups; four rifampicin- treated groups and two control groups. Rifampicin- treated groups were treated with a dose of either (27mg/kg/day) or (54mg/kg/day) and for each treatment dose, the treatment persists for either 14 days or 28 days. Certain parameters of sperm function including sperm concentration and sperm motility were assessed. Furthermore, analysis of sperm DNA integrity and chromatin quality were also studied. Results: No significant changes related to sperm concentration were observed when rifampicin was given in different doses and different durations. A significant change in sperm motility were recorded only when rifampicin was given in high dose for 28 days and there was a significant reduction in sperm progressive and total motility. Rifampicin showed a significant increase in sperm DNA staining capability when the dose and duration was increased. Administration of rifampicin in high dosage for 28 days represented in larger adverse impact on structure of sperm chromatin. Conclusion: Rifampicin could negatively affect male fertility potential in rats mainly through affecting the quality of sperm chromatin structure.

Download Full-text