scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

116. EFFECTS OF SUPPLEMENTAL VITAMIN ON THE SEMEN CHARACTERISTICS OF MARKHOZ GOATS DURING NON-BREEDING SEASON

2009 ◽  
Vol 21 (9) ◽  
pp. 35
Author(s):  
A. Towhidi ◽  
A. Farshad ◽  
M. Dolatpanah ◽  
R. Salehi
Keyword(s):  
Vitamin E ◽  
Breeding Season ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Supplemental Vitamin ◽  
Diet Control ◽  
Live Sperm

The aim of the study was to investigate the effect of dietary supplementation of vitamin E on the semen characteristics of the Markhoz goats. Eighth bucks were randomly allocated into two groups, and received two different diets: unsupplemented diet (control) and supplemented diet with vitamin E (0.30 g/Kg DM). All experimental diets were formulated according to AFRC 1998. Semen was collected at 14-days intervals from June 17, 2006 to September 2, 2006 (non-breeding season) using artificial vagina. Semen characteristics were evaluated including semen volume, sperm concentration, live sperm percentage, percentage of motility and progressive motility. This characteristics were evaluated at two weeks intervals the trial. The total number of spermatozoa per ejaculation was calculated by multiplication of the semen volume with sperm concentration. Sperm motility was also analyzed by placing a sample on pre-warmed (37 º C) microscope slide covered with a cover slip, and examined under a high power microscope at a magnification × 200. Data was analyzed using proc MIXED in SAS program. Significant effect (P≤0.05) of the week (sampling time) was observed for all the parameters except for semen volume. Vitamin E supplementation significantly improved (P≤0.05) total number (Control: 263.7 ×107±17.506 vs. Vit E: 320.95 ×107±17.506) and sperm concentration (Control: 301.79 ×107±13.657 vs. Vit E: 386.57 ×107± 13.657), motility (Control: 77.27% ±0.89 vs. Vit E: 82.6% ±0.89) and progressive motility (Control: 4.208 ±0.138 vs. Vit E: 4.229 ±0.138), percentage of viability (Control: 80.57% ±0.89 vs. Vit E: 85.9% ±0.89). The results suggested that the supplemental Vitamin E may improve the semen quality and fertility in the Markhoz goats.

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

scholarly journals PRELIMINARY OBSERVATIONS ON THE SEMINAL CHARACTERISTICS OF RABBITS RAISED IN TWO LOCATIONS IN SOUTHEASTERN NIGERIA.

2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Southeastern Nigeria ◽  
Abnormal Sperm ◽  
Port Harcourt ◽  
Semen Volume ◽  
Live Sperm ◽  
Artificial Vagina ◽  
Time Of Collection

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

scholarly journals Study on buck evaluation based on semen quality and fertility

2013 ◽  
Vol 42 (2) ◽  
pp. 101-108 ◽  
Author(s):  
F Sultana ◽  
SS Husain ◽  
A Khatun ◽  
AS Apu ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Fertility Data ◽  
Three Stages ◽  
Live Sperm

The experiment was conducted to evaluate the performance of buck on the basis of semen quality and fertility. Data on 162 ejaculates from five adult Black Bengal bucks (B1, B2, B3, B4 and B5) were recorded. Individual buck effect was found to be significant on semen volume (p<0.05), three stages of sperm motility (p<0.01) and percentage of live sperm (p<0.01) but insignificant (p>0.05) on sperm concentration and percentage of normal sperm. The results of semen volume per ejaculate, sperm concentration, initial sperm motility, on dilution sperm motility, post-thawing sperm motility, percentage of live sperm and percentage of normal sperm were ranged from 0.58±0.17 to 1.04±1.1 ml, 2827±0.76 to 3132

scholarly journals Feeding Treatment Based on Palm Oil Byproduct and Supplementation to Support Reproduction Performance of Bull

2016 ◽  
Vol 18 (3) ◽  
pp. 157
Author(s):  
Dian Ratnawati ◽  
Aryogi Aryogi
Keyword(s):  
Sperm Motility ◽  
Palm Oil ◽  
Protein Level ◽  
Semen Quality ◽  
Average Daily Gain ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Feed Consumption ◽  
Sperm Abnormality ◽  
Feed Protein

Palm oil waste (by product)can be used as a potential feed for livestock. Nevertheless, the study research of the effect of Palm oil waste (by product) as a feed to the bull performance was limited. The purpose of this research is to get technology to improve semen quality through improving protein of  feed based on palm oil waste (byproducts). This research was conducted in PTPN 6 Jambi and  used 30 bulls that separated into 3 treatments, treatment I (feed protein 12% and suplementation), treatment II (feed protein 12%) and treatment III (existing feed, feed protein 10%). Parameter were measured i.e feed consumption, libido, sperm motility, mass movement, sperm concentration, sperm abnormality, volume, pH, consistency, colour, body condition score and average daily gain. Design of this research was completely randomized design. Data was analyzed use ANOVA. The result showed that there is no significantly different on semen quality between treatmens. Semen quality of three treatments were appropriate to standart of quality semen of bull (sperm abnormality <20%, sperm motility >50% and sperm concentration >500 million/ml). Based on this consideration, feed with protein level 10% more efficient because it needs less cost but results a good semen quality. The conclusion of this research is protein level 10% can supporting performance reproduction of bull.

Sexual Behavior and its Relationship with Semen QualityParameters in Jaffrabadi Breeding Bulls

2019 ◽  
Vol 15 (01) ◽  
pp. 57-60
Author(s):  
J K Chaudhary ◽  
G B Solanki ◽  
H P Vijyeta ◽  
P M Gamit ◽  
K S Murthy
Keyword(s):  
Sexual Behavior ◽  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Quality Parameters ◽  
Mating Ability ◽  
Mean Values ◽  
Semen Volume ◽  
Mass Activity ◽  
Live Sperm

The present study was carried out on 80 ejaculates, 20 each from four healthy Jaffrabadi breeding bulls to see the relationship betweensexual behavior and semen quality parameters. The results depicted that the overall mean values of libido score, mating ability score andsexual libido score between bulls. The overall color of semen ranged from thin white to very thick creamy. The overall values for semen volume(ml), pH, sperm concentration (million/mL), mass activity, individual sperm motility (%) and live spermatozoa (%) were 2.99 ± 0.12, 6.80 ± 0.01, 1439.68 ± 48.46, 3.94 ± 0.03, 89.51 ± 0.21 and 95.00 ± 0.19, respectively. The values for semen volume, sperm concentration,mass activity (%) and live sperm (%) differed significantly (p less than 0.05) between bulls. There were significant positive correlations (p LESS THAN 0.01)of libido with mating ability, sexual behavior score and ejaculate volume of semen (r = 0.37 to 0.87), while a negative correlation wasfound with sperm concentration (-0.30). Mating ability was significantly (p less than 0.01) correlated with sexual behavior score and ejaculatevolume (0.78, 0.67), and sexual behavior was correlated with semen volume (0.84). Sperm concentration was significantly (p less than 0.05 correlated with mass activity (0.29), while individual sperm motility was positively (p less than 0.05) correlated with live sperm percentage (0.25).Correlations of libido, reflected the importance of sexual behavior of Jaffrabadi bulls in predicting their future utility as a proficient breeder under AI program.

Characteristics and freezability of Assam Hill goat semen

2017 ◽  
Author(s):  
S. Deori ◽  
B. C. Deka ◽  
R. K. Biswas ◽  
N. Nahardeka ◽  
A. Arangasamy ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Acceptable Quality ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
The Mean ◽  
Live Sperm

Assam Hill goat (AHG) is an important goat germplasm found in Assam and its adjoining areas of India. The study was designed with an objective to study the semen characteristics and freezability of AHG buck semen using Tris -Egg yolk-Citrate-Fructose diluent. The mean values of fresh semen characteristics in AHG bucks viz., ejaculate volume (ml), initial sperm motility (%), sperm concentration (x106/ml), live sperm (%), sperm abnormality (%), HOST-reacted sperm (%) and intact acrosome (%) recorded were 0.39 ± 0.01, 77.97 ± 0.73, 3201.00 ± 143.78, 83.02 ± 0.65, 7.66 ± 0.73, 66.95 ± 0.74 and 93.34 ± 0.51, respectively. Mean values for post-thaw semen characteristics i.e., sperm motility (%), live sperm (%), HOST-reacted sperm (%) and intact acrosome (%) were 55.39 ± 0.97, 71.01 ± 0.78, 54.77 ± 0.55 and 82.16 ± 0.43, respectively. It can be concluded that AHG bucks donate acceptable quality of semen which can be frozen successfully in Tris-Egg yolk-Citrate-Fructose diluents for using in Artificial Insemination.

scholarly journals Repeatability Estimates For Seminal Traits And Their Phenotypic Relationships With Testes Measurements And Performance Traits In Black Bengal Buck

1970 ◽  
Vol 37 (2) ◽  
pp. 34-41 ◽  
Author(s):  
AK Rajuana ◽  
MR Tayabur ◽  
MA Hoque ◽  
SS Husain ◽  
Z Sultana
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Body Condition ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Performance Traits ◽  
Mass Activity ◽  
And Performance

Repeatability for seminal traits and their phenotypic relationships with testes measurements and performance traits in Black Bengal bucks were estimated from a total of 116 repeated observations on 15 young Black Bengal bucks. Performance traits included age, body condition and body weight of bucks. Testes measurements were testes length, breadth and volume, and scrotal circumference, while seminal traits were ejaculate volume, semen density, mass activity, sperm motility, sperm concentration, total sperm per ejaculation and percent of normal sperm. High positive correlations (ranging from 0.81 to 0.90) were found between body weight and testes measurements. Semen volume and percent of normal sperm were positively correlated with age, body condition and body weight of bucks (ranging from 0.24 to 0.60). The testes measurements were strongly and positively correlated with semen volume and total sperm per ejaculation (ranging from 0.53 to 0.61), while the correlations between testes measurement and percent of normal sperm were moderate (ranging from 0.34 to 0.44). Among seminal traits, strong correlations were found between semen volume and total sperm per ejaculation (0.81) and, between mass activity and sperm motility (0.82). Repeatability of ejaculate volume was higher (r = 0.78) which indicated that selection or culling for semen ejaculate volume could be practiced from single or few observations. It could be concluded that bucks’ age, body weight and body condition along with testes volume should be considered as selection criteria for improving semen quality and semen production of breeding bucks. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9879 BJAS 2008; 37(2): 34-41

Semen characteristics of Awassi rams

1969 ◽  
Vol 73 (2) ◽  
pp. 311-314 ◽  
Author(s):  
K. H. Juma ◽  
F. Dessouky
Keyword(s):  
Methylene Blue ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Sperm Number ◽  
Sperm Production ◽  
College Of Agriculture ◽  
Sperm Abnormality ◽  
Methylene Blue Reduction ◽  
Semen Characteristics ◽  
Mass Activity

SUMMARYOn the College of Agriculture farm in Abu-Ghraib 147 ejaculates were collected from three docked and four normal Awassi rams born during November 1962. The work covered a period from 1 April 1967 to 31 March 1968.Nine semen characters, namely, volume, mass activity, individual motility, sperm concentration, sperm number, pH, methylene blue reduction time and the percentages of abnormal and dead sperms, were studied. The effect of season and docking on these characters was investigated.Seasonal variation was observed in all traits studied. On the whole, semen quality was poorest during winter and best during summer. The effect of docking was more pronounced on sperm concentration, sperm number and percentage of abnormal sperms. Docking appeared to increase sperm production and reduce sperm abnormality.

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