This study shows the bactericidal effect of Electromagnetic Field on fruit juice microbes. Short shelf-life period of fruit juice caused by spoilage organisms has limiting factor for its economy value. The Eighteen microorganisms isolated from both fresh and spoilt fruit samples (Pineapple and Apple), and identified during the study include, twelve (12) bacteria and Six (6) fungi, out of which only the bacterial isolates were exposed to electromagnetic field of 0mG, 500mG, and 5000mG for thirty minutes. The bacteria species were Leuconostoc mesentroides, Bacillus species, Lactobacillus brevis, Microbacterium species, Clostridium species, Bacillus cereus, Acetobacter aceti, and Staphylococcus aureus. The Gram negative bacteria isolates were Erwinia carotovora, Erwinia ananas, and Proteus species. Exposure of the isolates to an electromagnetic field of 0mG, 500mG and 5000mG showed a decrease in some electromagnetic field magnitude. This study shows reduction in growth range among most bacterial species tested at 500mG electromagnetic radiation exposure, but the growth of many of these bacterial species were triggered at 5000mG electromagnetic radiation exposure. This may mean an initiation of: adaptation mechanism, growth mechanism in some microorganism, and sugar content of the fruit juice from which they are being isolated. The exposure of the bacteria to electromagnetic field elicited detectable responses therefore depends on the adaptation mechanism of each bacteria and sugar content of the fruit from which it is being isolated from. Thus, future research can be done to optimize the limits specified for target microbes that are strength and frequency of this EMF in diseases control.