The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

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The Association between Ambient Temperature and Sperm Quality in Wuhan, China

10.21203/rs.2.24030/v1 ◽

2020 ◽

Author(s):

Xiaochen Wang ◽

Xiaojia Tian ◽

Bo Ye ◽

Yi Zhang ◽

Xiaotong Zhang ◽

...

Keyword(s):

Ambient Temperature ◽

Sperm Morphology ◽

Sperm Quality ◽

Interactive Effect ◽

Sperm Concentration ◽

Human Sperm ◽

Effect Of Temperature ◽

Pm 2.5 ◽

Normal Sperm ◽

Exposure Windows

Abstract Background Few epidemiological investigations have focused on the influence of environmental temperature on human sperm quality. Here, we evaluated the potential association between ambient temperature and human sperm quality in Wuhan, China, and examined the interactive effect of particulate matter (PM 2.5 ) and temperature. Methods 1780 males who had been living in Wuhan for no less than three months and received semen analysis at the Department of Reproductive Medicine in Renmin Hospital of Wuhan University between April 8, 2013 and June 30, 2015 were recruited. Daily mean meteorological data and air pollution data (PM 2.5 , O 3 and NO 2 ) in Wuhan between 2013 and 2015 were collected. A generalized linear model was used to explore the associations between ambient temperature and sperm quality (including sperm concentration, percentage of normal sperm morphology, and progressive motility) at 0–9, 10–14, 15–69, 70–90, and 0–90 days before semen examination, and the interaction between temperature and PM 2.5 . Results The associations between ambient temperature and sperm quality were an inverted U-shape at five exposure windows, except for a lag of 0–9 days for sperm concentration. A 1 °C increase in ambient temperature above the thresholds was associated with a 2.038 (1.292~2.783), 1.814 (1.217~2.411), 1.458 (1.138~1.777), 0.934(0.617~1.251) and 1.604 (1.258~1.951) decrease in the percentage of normal sperm morphology at lag 0–9, lag 10–14, lag 15–69, lag 70–90, and lag 0–90 days, respectively. The interaction p- values of PM 2.5 and temperature were mostly less than 0.05 at five exposure windows. When ambient temperature exposure levels were above the thresholds, a 0.979 (0.659-1.299) and 3.559 (0.251~6.867) decrease in percentage of normal sperm morphology per 1 °C increase in temperature at lag 0-90 days was observed in the PM 2.5 ≤ P 50 group and PM 2.5 > P 50 group, respectively. Conclusions Our results indicate that exposure to ambient temperature has a threshold effect on sperm quality, and PM 2.5 enhances the effect of temperature on sperm quality when temperatures are above the threshold.

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Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

Veterinary World ◽

10.14202/vetworld.2021.3156-3163 ◽

2021 ◽

pp. 3156-3163

Author(s):

Bongot Huaso Mulia ◽

Ardyta Widianti ◽

Jansen Manansang ◽

Dedi Rahmat Setiadi ◽

Vincentia Trisna Yoelinda ◽

...

Keyword(s):

Sperm Motility ◽

Sperm Morphology ◽

Total Concentration ◽

Sperm Concentration ◽

Panthera Pardus ◽

Semen Collection ◽

Normal Sperm ◽

Frozen Semen ◽

Semen Volume ◽

Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

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Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352005000600010 ◽

2005 ◽

Vol 57 (6) ◽

pp. 764-771

Author(s):

A.R. Silva ◽

R.C.S. Cardoso ◽

L.D.M. Silva

Keyword(s):

Liquid Nitrogen ◽

Sperm Motility ◽

Sperm Morphology ◽

Sperm Concentration ◽

Egg Yolk ◽

Tris Buffer ◽

Normal Sperm ◽

Manual Stimulation

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were observed between treatments after thawing regarding sperm motility and vigor, normal sperm morphology rate or longevity. Both dilution rates can be efficiently used for canine semen freezing.

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Impact of Seminal Plasma Trace Elements on Human Sperm Motility Parameters

Romanian Journal of Internal Medicine ◽

10.1515/rjim-2017-0034 ◽

2018 ◽

Vol 56 (1) ◽

pp. 15-20 ◽

Cited By ~ 3

Author(s):

Mohammad Mostakhdem Hashemi ◽

Nasser Behnampour ◽

Mojgan Nejabat ◽

Afsaneh Tabandeh ◽

Behrouz Ghazi-Moghaddam ◽

...

Keyword(s):

Trace Elements ◽

Sperm Motility ◽

Seminal Plasma ◽

Sperm Quality ◽

Semen Analysis ◽

Sperm Concentration ◽

Negative Relationship ◽

Human Sperm ◽

Computer Assisted ◽

Human Seminal Plasma

Abstract Introduction. Human seminal plasma contains a variety of macro and trace elements including magnesium (Mg), copper (Cu), zinc (Zn), and iron (Fe) that have essential roles in normal functioning of semen and its quality. The imbalance of these elements has been reported in several pathologic and male infertility disorders. Therefore, this study aimed to determine the levels of these elements in seminal plasma samples, their relationships with each other and their impact on sperm motility. Methods. Overall, 192 males (96 normospermic and 96 asthenospermic males) were enrolled in the study. Semen samples were collected by masturbation and computer-assisted/aided semen analysis of sperm motility was performed. The samples were centrifuged and seminal levels of Mg, Cu, Zn and Fe were measured using atomic absorption spectroscopy. Results. The levels of Zn did not differ between the two groups, while the levels of Mg, Cu, and Fe were significantly higher in normospermic males. Fe showed a positive correlation with Mg and Cu in asthenospermic group. However, a negative relationship was found between Mg and Fe levels and between Mg and sperm concentration in the normospermic group. Fe levels were higher in the normospermic group compared to the asthenospermic group. Nevertheless, increased Fe levels caused a decrease in most of sperm motility fractions. Conclusion: Elements play major roles in male fertility and directly affect sperm quality. According to the results of this study, the levels of Zn do not affect the sperm quality and motility, while Fe, Cu and Mg are decreased in males with sperm motility problems. Nevertheless, Fe levels can adversely affect sperm motility in normospermic men.

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Web- and Artificial Intelligence–Based Image Recognition For Sperm Motility Analysis: Verification Study (Preprint)

10.2196/preprints.20031 ◽

2020 ◽

Author(s):

Vincent FS Tsai ◽

Bin Zhuang ◽

Yuan-Hung Pong ◽

Ju-Ton Hsieh ◽

Hong-Chiang Chang

Keyword(s):

Artificial Intelligence ◽

Sperm Motility ◽

Image Recognition ◽

Sperm Quality ◽

Sperm Concentration ◽

Human Sperm ◽

Recognition Algorithm ◽

Motile Sperm ◽

Home Testing ◽

System P

BACKGROUND Human sperm quality fluctuates over time. Therefore, it is crucial for couples preparing for natural pregnancy to monitor sperm motility. OBJECTIVE This study verified the performance of an artificial intelligence–based image recognition and cloud computing sperm motility testing system (Bemaner, Createcare) composed of microscope and microfluidic modules and designed to adapt to different types of smartphones. METHODS Sperm videos were captured and uploaded to the cloud with an app. Analysis of sperm motility was performed by an artificial intelligence–based image recognition algorithm then results were displayed. According to the number of motile sperm in the vision field, 47 (deidentified) videos of sperm were scored using 6 grades (0-5) by a male-fertility expert with 10 years of experience. Pearson product-moment correlation was calculated between the grades and the results (concentration of total sperm, concentration of motile sperm, and motility percentage) computed by the system. RESULTS Good correlation was demonstrated between the grades and results computed by the system for concentration of total sperm (r=0.65, P<.001), concentration of motile sperm (r=0.84, P<.001), and motility percentage (r=0.90, P<.001). CONCLUSIONS This smartphone-based sperm motility test (Bemaner) accurately measures motility-related parameters and could potentially be applied toward the following fields: male infertility detection, sperm quality test during preparation for pregnancy, and infertility treatment monitoring. With frequent at-home testing, more data can be collected to help make clinical decisions and to conduct epidemiological research.

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Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/3686494 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Guang Yan ◽

Fang Tian ◽

Peng Liu ◽

Jianming Sun ◽

Jianmin Mao ◽

...

Keyword(s):

Plasma Membrane ◽

Sperm Motility ◽

Sperm Morphology ◽

Sperm Concentration ◽

Membrane Integrity ◽

Testicular Tissue ◽

Whole Body ◽

Plasma Membrane Integrity ◽

Normal Sperm ◽

Sperm Plasma Membrane

Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.

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Web- and Artificial Intelligence–Based Image Recognition For Sperm Motility Analysis: Verification Study

JMIR Medical Informatics ◽

10.2196/20031 ◽

2020 ◽

Vol 8 (11) ◽

pp. e20031

Author(s):

Vincent FS Tsai ◽

Bin Zhuang ◽

Yuan-Hung Pong ◽

Ju-Ton Hsieh ◽

Hong-Chiang Chang

Keyword(s):

Artificial Intelligence ◽

Sperm Motility ◽

Image Recognition ◽

Sperm Quality ◽

Sperm Concentration ◽

Human Sperm ◽

Recognition Algorithm ◽

Testing System ◽

Motile Sperm ◽

Home Testing

Background Human sperm quality fluctuates over time. Therefore, it is crucial for couples preparing for natural pregnancy to monitor sperm motility. Objective This study verified the performance of an artificial intelligence–based image recognition and cloud computing sperm motility testing system (Bemaner, Createcare) composed of microscope and microfluidic modules and designed to adapt to different types of smartphones. Methods Sperm videos were captured and uploaded to the cloud with an app. Analysis of sperm motility was performed by an artificial intelligence–based image recognition algorithm then results were displayed. According to the number of motile sperm in the vision field, 47 (deidentified) videos of sperm were scored using 6 grades (0-5) by a male-fertility expert with 10 years of experience. Pearson product-moment correlation was calculated between the grades and the results (concentration of total sperm, concentration of motile sperm, and motility percentage) computed by the system. Results Good correlation was demonstrated between the grades and results computed by the system for concentration of total sperm (r=0.65, P<.001), concentration of motile sperm (r=0.84, P<.001), and motility percentage (r=0.90, P<.001). Conclusions This smartphone-based sperm motility test (Bemaner) accurately measures motility-related parameters and could potentially be applied toward the following fields: male infertility detection, sperm quality test during preparation for pregnancy, and infertility treatment monitoring. With frequent at-home testing, more data can be collected to help make clinical decisions and to conduct epidemiological research.

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The outcome of intra-cytoplasmic sperm injection (ICSI): do the sperm concentration and motility matter?

Hormone Molecular Biology and Clinical Investigation ◽

10.1515/hmbci-2020-0089 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ai Chen Chua ◽

Abdul Kadir Abdul Karim ◽

Albert Chao Chiet Tan ◽

Muhammad Azrai Abu ◽

Mohd Faizal Ahmad

Keyword(s):

Pregnancy Outcome ◽

Sperm Motility ◽

Sperm Concentration ◽

Group Iv ◽

Pregnancy Rates ◽

University Hospital ◽

Male Factor ◽

Normal Sperm ◽

Group I ◽

Group Ii

Abstract Objectives The male-factor subfertility was reported to be 30% globally; thus, the intracytoplasmic sperm injection (ICSI) procedure was implemented to improve the overall in vitro fertilization (IVF) rates. Nevertheless, at least 10% of fertilization failure still occurs. Concerning this issue, we explored the association of sperm concentration and motility with the quality of embryo development and pregnancy outcome in IVF-ICSI cycles. Methods Retrospective analysis of 109 couples with male factor were done over 14 months in a tertiary university hospital in Malaysia. The data were divided into four groups; Group I: normal sperm parameters, Group II: normal sperm concentration but reduced total sperm motility, Group III: reduced sperm concentration and motility, Group IV: reduced sperm concentration but normal sperm motility. Only fresh semen samples and fresh embryo transfers were included. The fertilization, cleavage rate, embryo quality and pregnancy outcome were assessed. Results Overall, group I had the highest oocytes yield and ICSI attempted; (10.12 + 6.50), whereas the lowest was in group IV; (7.00 + 2.82). Group II revealed the highest fertilization and cleavage rates; (54.14 + 25.36), (55.16 + 26.06), thus not surprisingly resulting in the highest number of good embryos and highest clinical pregnancy rates. The lowest cleavage and pregnancy rates were seen in group IV. However, all the outcomes were not statically significant (p>0.05). Conclusions Similar fertilization rate and comparable pregnancy outcome was seen among couples with normal and reduced sperm concentration and motility.

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The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats

World Nutrition Journal ◽

10.25220/wnj.v01i1.0007 ◽

2017 ◽

Vol 1 (1) ◽

pp. 28

Author(s):

Eviana Budiartanti Sutanto ◽

Taufiq R Nasihun ◽

Israhnanto Isradji ◽

Luciana Budiati Sutanto

Keyword(s):

Vitamin C ◽

Sperm Quality ◽

Sperm Concentration ◽

Control Group ◽

Control Group Design ◽

Normal Sperm ◽

Post Test ◽

Male Wistar Rats ◽

Post Hoc ◽

Per Oral

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.

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Assessment of Sperm Quality - A Light Microscope Study

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/299 ◽

2021 ◽

Vol 10 (19) ◽

pp. 1417-1421

Author(s):

Jyothi A. Raj ◽

Heera Sankar ◽

Sagarika Mahapatra ◽

Ashima Binny

Keyword(s):

Light Microscope ◽

Sperm Morphology ◽

Clinical Laboratory ◽

Sperm Quality ◽

Semen Analysis ◽

Complete Evaluation ◽

Normal Sperm ◽

Middle Piece ◽

Sperm Counts ◽

Work Up

BACKGROUND Semen analysis is an integral part of work up for infertility in men, with sperm morphology being an important qualitative parameter. Qualitative defects can affect any part of the sperm and are classified as defects in the head, middle piece, and tail, based on morphology. The focus of the study was to assess qualitative defects in sperms by light microscopy, in semen with normal sperm counts. METHODS This study is hospital based, descriptive, retrospective study. Of the semen samples received in the clinical laboratory, fifty with normal sperm counts were included in the study and processed according to standard protocol. For evaluation of qualitative defects by sperm morphology, smears were fixed in ethanol, stained with Papanicolaou stain [PAP], and assessed under light microscope. RESULTS The 50 semen samples included in the study had sperm counts ranging from 15 to 80 million / ml. Thirty samples had less than 10 % abnormal forms, fourteen samples had 11 - 20 % abnormal forms, five samples had 21 - 30 % abnormal forms and one sample had 40 % abnormal sperms. Qualitative defects were classified as morphological abnormalities in head, neck, and tail. Of the fifty cases, most defects were found in the head, followed by those in the neck and tail. Common defects noted were double head (44 %), abnormal sized heads, and bent neck (48 %). Coiling was a common defect noted in the tail (10 %). Most sperms showed a combination of defects. CONCLUSIONS Qualitative defects in sperm morphology are often seen in samples with normal sperm counts. Assessment of microscopic characteristics of human spermatozoa is as important as count and motility in the complete evaluation and work-up of semen samples in cases of infertility. KEY WORDS Semen, Sperm, Quality, Microscopy, Morphology

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