Immunogold staining method for the light microscopic detection of leukocyte cell surface antigens with monoclonal antibodies: its application to the enumeration of lymphocyte subpopulations.

1983 ◽  
Vol 31 (3) ◽  
pp. 376-381 ◽  
Author(s):  
M De Waele ◽  
J De Mey ◽  
M Moeremans ◽  
M De Brabander ◽  
B Van Camp
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Peripheral Blood ◽  
Colloidal Gold ◽  
Staining Method ◽  
Surface Antigens ◽  
Lymphocyte Subpopulations ◽  
Cell Surface Antigens ◽  
Immunogold Staining ◽  
Microscopic Detection

Colloidal gold was used as a marker for the light microscopic detection of lymphocyte cell surface antigens with monoclonal antibodies. Suspensions of peripheral blood leukocytes were first incubated with monoclonal mouse antibodies and then with colloidal gold-labeled goat anti-mouse antibodies. The cells were fixed and cytocentrifuge preparations or smears were made. Granulocytes and monocytes were then labeled by the cytochemical staining of their endogenous peroxidase activity. Lymphocytes reacting with the monoclonal antibody had numerous dark granules around the surface membrane. With electron microscopy, these granules appeared as patches of gold particles. This immunogold staining method proved to be a reliable tool for the enumeration of T-lymphocyte subpopulations in peripheral blood. The results were almost identical to those obtained with immunofluorescence microscopy. The procedure can also be applied on small volumes of capillary blood. This constitutes a good microtechnique for the determination of lymphocyte subsets in children.

The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

1985 ◽  
Vol 76 (2) ◽  
pp. 211-222 ◽  
Author(s):  
Ph. Coppe ◽  
J.-J. Letesson ◽  
M. Saint-Guillain ◽  
R. Leloup
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Colloidal Gold ◽  
Surface Antigens ◽  
Cell Surface Antigens

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Analysis of cell surface antigens on glucocorticoid-treated rat thymocytes with monoclonal antibodies

1994 ◽  
Vol 39 (3) ◽  
pp. 209-217 ◽  
Author(s):  
M. Tsuchida ◽  
M. Konishi ◽  
K. Jojima ◽  
K. Naito ◽  
Y. Fujikura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Surface Antigens ◽  
Cell Surface Antigens

Protective antigens of bloodstage Plasmodium knowlesi parasites

1984 ◽  
Vol 307 (1131) ◽  
pp. 159-169 ◽  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Plasmodium Knowlesi ◽  
Surface Antigens ◽  
Red Cell ◽  
Cell Surface Antigens ◽  
Susceptible Host ◽  
Protective Antigens ◽  
Stage Of Development

The simian malaria Plasmodium knowlesi provides many favourable features as an experimental model; it can be grown in vivo or in vitro . Parasites of defined variant specificity and stage of development are readily obtained and both the natural host and a highly susceptible host are available for experimental infection and vaccination trials. Proteins synthesized by erythrocytic P. knowlesi parasites are characteristic of the developmental stage, as are the alterations that the parasite induces in the red cell surface. Erythrocytic merozoites are anatomically and biochemically complex, their surface alone is covered by at least eight distinct polypeptides. Immune serum from merozoite-immunized rhesus recognizes many parasite components, especially those synthesized by schizonts. All of the merozoite surface components and some of the schizont-infected red cell surface antigens are recognized by such immune sera. Rhesus monkeys rendered immune by repeated infection may by contrast recognize comparatively few antigens; a positive correlation was established for these ‘ naturally ’ immunized monkeys between protection and antibody directed against a 74000 molecular mass antigen. Im m unization with this purified antigen confers partial protection. O ther putative protective antigens have been identified by monoclonal antibodies that inhibit merozoite invasion of red cells in vitro . The antigens recognized by inhibitory monoclonal antibodies are synthesized exclusively by schizonts and are processed, at the time ofschizont rupture and merozoite release, to smaller molecules that are present on the merozoite surface. The multiplicity of protective antigens is clearly demonstrated by the fact that seven distinct merozoite surface antigens are recognized by three different inhibitory monoclonals. None of the protective antigens identified are variant or strain specific.

Monoclonal antibodies to tissue-specific cell surface antigens

1984 ◽  
Vol 33 (2) ◽  
pp. 268-281 ◽  
Author(s):  
Ann M. Carroll ◽  
Michael Zalutsky ◽  
Sam Schatten ◽  
Atul Bhan ◽  
Linda L. Perry ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Surface Antigens ◽  
Specific Cell ◽  
Cell Surface Antigens ◽  
Tissue Specific ◽  
Specific Cell Surface

scholarly journals Flow Cytometric Analysis of T, B, and NK Cells Antigens in Patients with Mycosis Fungoides

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Serkan Yazıcı ◽  
Emel Bülbül Başkan ◽  
Ferah Budak ◽  
Barbaros Oral ◽  
Şaduman Balaban Adim ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mycosis Fungoides ◽  
Peripheral Blood ◽  
Prognostic Value ◽  
Flow Cytometric Analysis ◽  
Surface Antigens ◽  
Cell Surface Antigens ◽  
Flow Cytometric ◽  
Hla Dr ◽  
The Mean

We retrospectively analyzed the clinicopathological correlation and prognostic value of cell surface antigens expressed by peripheral blood mononuclear cells in patients with mycosis fungoides (MF). 121 consecutive MF patients were included in this study. All patients had peripheral blood flow cytometry as part of their first visit. TNMB and histopathological staging of the cases were retrospectively performed in accordance with International Society for Cutaneous Lymphomas/European Organization of Research and Treatment of Cancer (ISCL/EORTC) criteria at the time of flow cytometry sampling. To determine prognostic value of cell surface antigens, cases were divided into two groups as stable and progressive disease. 17 flow cytometric analyses of 17 parapsoriasis (PP) and 11 analyses of 11 benign erythrodermic patients were included as control groups. Fluorescent labeled monoclonal antibodies were used to detect cell surface antigens: T cells (CD3+, CD4+, CD8+, TCRαβ+, TCRγδ+, CD7+, CD4+CD7+, CD4+CD7−, and CD71+), B cells (HLA-DR+, CD19+, and HLA-DR+CD19+), NKT cells (CD3+CD16+CD56+), and NK cells (CD3−CD16+CD56+). The mean value of all cell surface antigens was not statistically significant between parapsoriasis and MF groups. Along with an increase in cases of MF stage statistically significant difference was found between the mean values of cell surface antigens. Flow cytometric analysis of peripheral blood cell surface antigens in patients with mycosis fungoides may contribute to predicting disease stage and progression.

Sign in / Sign up

Export Citation Format

Share Document