In Vitro Studies on the Anemia of Tumor-Bearing Hamsters

Abstract 1. Sterile, cell-free extracts of the viable portion of a methylcholanthrene-induced sarcoma of the hamster were capable of hemolyzing in vitro hamster red blood cells from the donor animals, and from animals with homologous and heterologous tumors. 2. Sterile, cell-free extracts of the necrotic material from this same tumor had little in vitro hemolytic action. 3. Whole tumor extracts varied in their in vitro hemolytic activity depending upon the proportion of viable to necrotic tissue present, with the maximum hemolysis observed when the whole tumor contained more viable than necrotic tissue. 4. Sterile, cell-free extracts of normal hamster liver had a strong hemolytic action on a whole range of red blood cells. 5. Hemolysins elaborated by the viable tissue in transplanted hamster tumors may be one factor contributing to the anemia in hamsters bearing transplantable sarcomas.

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In vitro hemolytic activity ofPlasmodium berghei on red blood cells

Journal of Biosciences ◽

10.1007/bf02703491 ◽

1980 ◽

Vol 2 (2) ◽

pp. 129-134 ◽

Cited By ~ 1

Author(s):

Sudhir Gupta ◽

K. C. Saxena

Keyword(s):

Red Blood Cells ◽

Hemolytic Activity ◽

Blood Cells

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THE PRODUCTION OF PURPURA BY DERIVATIVES OF PNEUMOCOCCUS

Journal of Experimental Medicine ◽

10.1084/jem.43.1.111 ◽

1926 ◽

Vol 43 (1) ◽

pp. 111-106

Author(s):

Hobart A. Reimann ◽

Louis A. Julianelle

Keyword(s):

Red Blood Cells ◽

Hemolytic Activity ◽

Blood Cells ◽

Blood Platelets ◽

White Blood Cells ◽

Red Cells ◽

Severe Anemia ◽

Marked Diminution ◽

Derivatives Of

A study has been made of the variation in number of the blood platelets, and the red and white blood cells of white mice injected with pneumococcus extract. The blood platelets were greatly diminished after the injection, the greatest decrease usually occurring after 24 hours. Purpuric lesions usually developed when the number of blood platelets became less than 500,000 per c.mm. Regeneration of the platelets was accomplished by the 4th to the 9th day but there was an overregeneration and the return to normal did not take place until 2 weeks had elapsed. The red cells were also greatly reduced in number, but the rate of their destruction and regeneration was somewhat slower than that of the platelets. The leucocytes were slightly if at all influenced by the pneumococcus extract. Pneumococcus extracts were shown to be thrombolytic and hemolytic. Heat destroyed the activity of both the lysins in vitro. Heated extract produced purpura in mice but did not cause a severe anemia. Extracts adsorbed with either blood platelets or red blood cells showed a marked diminution in their thrombolytic and hemolytic activity in vitro. Such extracts, however, produced purpura as well as severe anemia and thrombopenia in mice.

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In vitro studies on the entry of polyamines into normal red blood cells

Biochimie ◽

10.1016/0300-9084(84)90022-1 ◽

1984 ◽

Vol 66 (5) ◽

pp. 385-393 ◽

Cited By ~ 31

Author(s):

Jacques-Phillippe Moulinoux ◽

Michèle Le Calve ◽

Véronique Quemener ◽

Gérard Quash

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

In Vitro Studies

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Genetically engineered pig red blood cells for clinical transfusion: initial in vitro studies

Transfusion ◽

10.1111/j.1537-2995.2009.02306.x ◽

2009 ◽

Vol 49 (11) ◽

pp. 2418-2429 ◽

Cited By ~ 21

Author(s):

Cassandra Long ◽

Hidetaka Hara ◽

Zachary Pawlikowski ◽

Naoko Koike ◽

Thomas d'Arville ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Genetically Engineered ◽

In Vitro Studies

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IN VITRO STUDIES WITH 16-C14-ESTRONE: DISTRIBUTION BETWEEN PLASMA AND RED BLOOD CELLS OF MAN*

Journal of Clinical Investigation ◽

10.1172/jci103839 ◽

1959 ◽

Vol 38 (4) ◽

pp. 611-618 ◽

Cited By ~ 10

Author(s):

Patricia E. Wall ◽

Claude J. Migeon

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

In Vitro Studies

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Alteration of human red blood cells stored in plastic packs. A case report with in vivo and in vitro studies

Transfusion ◽

10.1046/j.1537-2995.1982.22282177125.x ◽

1982 ◽

Vol 22 (2) ◽

pp. 154-157 ◽

Cited By ~ 4

Author(s):

JT Callahan ◽

MF Collecutt ◽

JR Lightbody ◽

BS Faragher

Keyword(s):

Case Report ◽

Red Blood Cells ◽

Blood Cells ◽

In Vitro Studies ◽

Human Red Blood Cells

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HEMOLYSIS WITH ALLOXAN AND ALLOXAN-LIKE COMPOUNDS, AND THE PROTECTIVE ACTION OF TOCOPHEROL

Blood ◽

10.1182/blood.v5.11.1062.1062 ◽

1950 ◽

Vol 5 (11) ◽

pp. 1062-1074 ◽

Cited By ~ 60

Author(s):

CATHARINE S. ROSE ◽

PAUL GYÖRGY

Keyword(s):

Ascorbic Acid ◽

Blood Cell ◽

Red Blood Cells ◽

Blood Cells ◽

Protective Action ◽

Antioxidant Effect ◽

Oxidation Reduction ◽

Hemolytic Action

Abstract The production of hemolysis in tocopherol-deficient rats by injection of compounds related to alloxan has been studied. Alloxantin had the same hemolytic action as alloxan; dialuric acid was about twice, ninhydrin about six times as effective as alloxan. Alloxanic acid had no hemolytic action. In tocopherol-treated rats no hemolysis was observed after the injection of alloxantin, dialuric acid and ninhydrin, as well as of alloxan. Hemolysis of red blood cells obtained from tocopherol-deficient rats could be produced in vitro by dialuric acid and to a lesser extent by alloxantin but not by alloxan or ninhydrin. In the presence of cysteine, glutathione or ascorbic acid, alloxan reacted as dialuric acid. The three reducing agents themselves hemolyzed the cells of deficient rats although to a much lesser extent than did dialuric acid. The red blood cells of tocopherol-treated rats were resistant to hemolysis in vitro as in vivo by all the above hemolyzing compounds. Addition of tocopherol to the reaction mixture protected the cells of deficient animals against the hemolytic action of dialuric acid. The tocopherol was more effective when it was incubated with the cells before the dialuric acid was added. It is proposed that the hemolysis is linked with the reversible oxidation-reduction system of dialuric acid and alloxan, the actual hemolyzing agent being an intermediate of this reaction. Cysteine, glutathione and ascorbic acid, which are readily oxidized by molecular oxygen may form similar intermediates. The protective action of tocopherol is a function of the red blood cell and may best be explained as an antioxidant effect.

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Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162132 ◽

1990 ◽

Vol 48 (3) ◽

pp. 914-915

Author(s):

D.J.P. Ferguson ◽

A.R. Berendt ◽

J. Tansey ◽

K. Marsh ◽

C.I. Newbold

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Umbilical Vein ◽

Cell Types ◽

Amelanotic Melanoma ◽

Cytoplasmic Process ◽

Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

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