scholarly journals Studies on Congenital Hemolytic Syndromes. IV. Gastrointestinal Absorption of Iron

Blood ◽  
1962 ◽  
Vol 19 (3) ◽  
pp. 359-378 ◽  
Author(s):  
MARION E. ERLANDSON ◽  
BEATRICE WALDEN ◽  
GERTRUDE STERN ◽  
MARGARET W. HILGARTNER ◽  
JACQUELINE WEHMAN ◽  
...  
Keyword(s):  
Sickle Cell ◽  
Serum Iron ◽  
Binding Capacity ◽  
Specific Effect ◽  
Thalassemia Major ◽  
Gastrointestinal Absorption ◽  
Iron Binding ◽  
Normal Children ◽  
Hemoglobin C ◽  
Iron Binding Capacity

Abstract 1. Results of studies of gastrointestinal absorption of ferrous iron in normal children and those with heterozygous thalassemia were similar. 2. In one patient with absent erythropoiesis but severe anemia, no increase in the amount of iron absorbed was noted. 3. In sickle cell-hemoglobin C disease and hereditary spherocytosis having only slight anemia in the presence of increased erythropoiesis, normal amounts of iron were absorbed. 4. Patients with sickle cell anemia and thalassemia major in whom there was active erythropoiesis and marked anemia absorbed abnormally large amounts of iron. The amount absorbed by individuals with the latter disease could be reduced by administration of transfusions and concomitant suppression of erythropoiesis. 5. Usual values for serum iron and latent iron-binding capacity in several congenital hemolytic syndromes have been presented and their significance discussed. 6. No specific effect on absorption was noted by increased or reduced amounts of tissue or serum iron or by reduced or increased latent iron-binding protein.

SERUM IRON AND IRON-BINDING CAPACITY OF THE SERUM IN CHILDREN WITH SEVERE MEDITERRANEAN (COOLEY'S) ANEMIA

PEDIATRICS ◽  
1950 ◽  
Vol 5 (5) ◽  
pp. 799-807
Author(s):  
CARL H. SMITH ◽  
THOMAS R. C. SISSON ◽  
WILLIAM H. FLOYD ◽  
SHIRLEY SIEGAL
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Binding Protein ◽  
High Serum ◽  
Total Iron ◽  
Deficiency Anemia ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Normal Children ◽  
Iron Binding Capacity

The blood of children with severe Mediterranean anemia was found to have a high serum iron and an absence of a latent or unbound iron capacity of the serum. Whereas serum iron levels comprise approximately one third of the total iron-binding capacity in normal children and adults, the two values are equal in the severe form of Mediterranean anemia. The iron-binding capacity of the serum in this condition is therefore fully saturated and quantitatively lower than in normal children. The absence of a measurable latent iron-binding capacity does not depend entirely on the liberation of iron from exogenously administered blood. Comparable changes were also noted in two nonanemic adult carriers possessing the trait of the disease, in two children with the mild form not requiring transfusions, and in one severely anemic child with Mediterranean anemia who had never been transfused. Similar changes occurred in three patients with spherocytic anemia and in five children with sickle cell anemia, one of whom had never been transfused. In every instance therefore of the advanced form of Mediterranean anemia and in some of the nonanemic and mildly anemic forms of this disease, a fully saturated metal-binding protein is present in total amounts usually less than those found in normal children. In contrast, children with iron deficiency anemia manifest a reduction in percentage saturation of iron-binding protein, an increased level of unbound latent iron-binding capacity and a markedly elevated total iron-binding capacity. The failure to demonstrate a latent iron-binding capacity of the serum in asymptomatic persons adds another criterion to those already established for the identification of carriers of the trait of Mediterranean anemia who constitute the source of its transmission. The presence of bilirubin in increased concentrations in the serum of patients with severe Mediterranean anemia does not interfere with its capacity to combine with iron. The capacity for binding iron was restored when Fraction IV-7 was added to samples of icteric sera of patients in whom no capacity had previously been demonstrated. The high serum iron and absence of latent iron-binding capacity observed in children with severe Mediterranean anemia receiving multiple transfusions correspond to the changes regarded as diagnostic of transfusion hemosiderosis and hemochromatosis. While these changes remain unaltered in Mediterranean anemia, their reversibility has been demonstrated in two children with hemophilia who received weekly transfusions of small amounts of blood. Following cessation of this form of therapy a decrease of serum iron and a gradual restoration of iron-binding capacity were noted.

scholarly journals Interference of deferasirox with assays for serum iron and serum unsaturated iron binding capacity during iron chelating therapy

2011 ◽  
Vol 412 (23-24) ◽  
pp. 2261-2266 ◽  
Author(s):  
Katsuya Ikuta ◽  
Satoshi Ito ◽  
Hiroki Tanaka ◽  
Katsunori Sasaki ◽  
Yoshihiro Torimoto ◽  
...  
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Binding ◽  
Iron Binding Capacity ◽  
Chelating Therapy

Serum Iron and Serum Iron-Binding Capacity

1963 ◽  
pp. 139-150 ◽  
Author(s):  
THOMAS J. GIOVANNIELLO ◽  
THEODORE PETERS ◽  
EDWARD WAGMAN
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Iron Binding ◽  
Iron Binding Capacity

Serum iron and total iron-binding capacity compared with serum ferritin in assessment of iron deficiency.

1981 ◽  
Vol 27 (2) ◽  
pp. 276-279 ◽  
Author(s):  
F Peter ◽  
S Wang
Keyword(s):  
Iron Deficiency ◽  
Serum Ferritin ◽  
Serum Iron ◽  
Binding Capacity ◽  
Transferrin Saturation ◽  
Total Iron ◽  
Iron Binding ◽  
Total Iron Binding Capacity ◽  
Iron Deficient ◽  
Iron Binding Capacity

Abstract Ferritin values for 250 selected sera were compared with values for iron, total iron-binding capacity (TIBC), and transferrin saturation, to assess the potential of the ferritin assay for the detection of latent iron deficiency. The specimens were grouped (50 in each group) according to their values for iron and TIBC. In Group 1 (low iron, high TIBC) the saturation and ferritin values both indicated iron deficiency in all but one. In the 100 specimens of Groups 2 (normal iron, high TIBC) and 4 (normal iron, high normal TIBC), the saturation values revealed 16 iron-deficient cases, the ferritin test 55. For Groups 3 (low iron, normal TIBC) and 5 (low iron, low TIBC), the ferritin test revealed fewer cases of iron deficiency than did the saturation values (37 cases vs 51 cases, in the 100 specimens). Evidently the ferritin test detects iron deficiency in many cases for whom the serum iron and TIBC tests are not positively indicative. The correlation of serum ferritin with iron, TIBC, and transferrin saturation in the five groups was good only in the case of specimens for which the TIBC was normal; if it was abnormal the correlation was very poor.

Use of an Anion-Exchange Column to Determine Serum Iron-Binding Capacity

1971 ◽  
Vol 17 (9) ◽  
pp. 941-947 ◽  
Author(s):  
H Peter Lehmann ◽  
Alex Kaplan
Keyword(s):  
Serum Iron ◽  
Binding Capacity ◽  
Ferric Citrate ◽  
Iron Binding ◽  
Resin Column ◽  
Anion Exchange Column ◽  
Excess Iron ◽  
Colorimetric Measurement ◽  
Color Reagent ◽  
Iron Binding Capacity

Abstract A rapid procedure is described for determining total iron-binding capacity (TIBC) by use of a small, disposable resin column. Serum is saturated with excess ferric citrate, and iron not bound to protein is then removed by passing the mixture through the column of anion-exchanger. Transferrin-bound iron in the effluent, the TIBC, is determined colorimetrically (AutoAnalyzer), with 2,4,6-tripyridyl-1,3,5-triazine as color reagent. The percentage saturation with iron may be obtained from colorimetric measurement of original serum iron and the TIBC, or by radioactive counting if 59Fe is incorporated in the ferric citrate used to saturate the serum. Our method is compared with the MgCO3 precipitation technique for the removal of excess iron after saturation and to an ultrafiltration technique in which protein-bound iron is retained by a membrane and the filtrate analyzed for unbound iron, thus obviating absorbents in the determination of TIBC. Results from the resin-column and ultrafiltration procedures correlated well, whereas those from the MgCO3 method were consistently lower and more erratic.

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