scholarly journals The Philadelphia chromosome in human macrophages

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 367-370 ◽  
Author(s):  
DW Golde ◽  
C Burgaleta ◽  
RS Sparkes ◽  
MJ Cline
Keyword(s):  
Bone Marrow ◽  
Natural History ◽  
Cell Line ◽  
Bone Marrow Cells ◽  
Philadelphia Chromosome ◽  
Mononuclear Phagocyte ◽  
Chronic Myelocytic Leukemia ◽  
Human Macrophages ◽  
Myelocytic Leukemia ◽  
History Of

Three patients with chronic myelocytic leukemia in different phases of the natural history of the disease were studied. Their bone marrow cells were cultured under conditions favoring macrophage proliferation, and parallel cytogenetic and cytochemical studies were performed. All cell metaphases examined contained the Ph1 chromosome at a time when more than 80% of these metaphases were in identifiable macrophages. We conclude that the mononuclear phagocyte cell line contains the abnormal chromosome in Ph1-positive chronic myelocytic leukemia.

scholarly journals The Philadelphia chromosome in human macrophages

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 367-370 ◽  
Author(s):  
DW Golde ◽  
C Burgaleta ◽  
RS Sparkes ◽  
MJ Cline
Keyword(s):  
Bone Marrow ◽  
Natural History ◽  
Cell Line ◽  
Bone Marrow Cells ◽  
Philadelphia Chromosome ◽  
Mononuclear Phagocyte ◽  
Chronic Myelocytic Leukemia ◽  
Human Macrophages ◽  
Myelocytic Leukemia ◽  
History Of

Abstract Three patients with chronic myelocytic leukemia in different phases of the natural history of the disease were studied. Their bone marrow cells were cultured under conditions favoring macrophage proliferation, and parallel cytogenetic and cytochemical studies were performed. All cell metaphases examined contained the Ph1 chromosome at a time when more than 80% of these metaphases were in identifiable macrophages. We conclude that the mononuclear phagocyte cell line contains the abnormal chromosome in Ph1-positive chronic myelocytic leukemia.

scholarly journals Natural history of mixed chimerism after bone marrow transplantation with CD6-depleted allogeneic marrow: a stable equilibrium

Blood ◽  
1990 ◽  
Vol 75 (1) ◽  
pp. 296-304 ◽  
Author(s):  
DC Roy ◽  
R Tantravahi ◽  
C Murray ◽  
K Dear ◽  
B Gorgone ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Natural History ◽  
Marrow Transplantation ◽  
Cytogenetic Analysis ◽  
Common Finding ◽  
Mixed Chimerism ◽  
Allogeneic Bone ◽  
Donor Cells ◽  
History Of

Mixed hematopoietic chimerism (MC) is a common finding after allogeneic bone marrow transplantation (BMT), but the natural history of this phenomenon remains unclear. To understand the evolution and the implications of this finding, we performed a prospective analysis of the development of mixed chimerism in 43 patients with hematologic malignancies who received bone marrow (BM) from human leukocyte antigen (HLA)-identical sibling donors. T-cell depletion in vitro with anti-T12 (CD6) monoclonal antibody and rabbit complement was used as the only method of graft-versus-host disease (GVHD) prophylaxis. Overall, MC was identified in peripheral blood (PB) and BM in 22 of 43 (51%) patients evaluated. MC was found by restriction fragment length polymorphism (RFLP) analysis in 21 of 40 (53%) patients, by cytogenetic analysis in 6 of 29 (21%) patients, and by red blood cell phenotyping in 4 of 9 (44%) patients. RFLP studies were performed at 0.5, 1, 3, 6, 9, and 12 months post-BMT and then every 6 months, and showed a high probability of developing MC in the first 6 months after BMT followed by stabilization after 12 months. Cytogenetic analysis was less sensitive in detecting MC. Once MC was detected after BMT, the percentage of recipient cells increased very slowly over more than 3 years of follow- up, and no patient reverted to complete donor hematopoiesis (CDH). Thus, recipient and donor cells remained in a relative state of equilibrium for prolonged periods that seemed to favor recipient cells over donor cells. Patient's disease, remission status, or intensity of the transplant preparative regimen did not influence the subsequent development of mixed chimerism. Early immunologic reconstitution was the only factor that correlated with the subsequent chimeric status of the patients. The percentage and absolute number of T3 (CD3) and T4 (CD4) positive cells at day 14 after BMT were significantly higher in the patients who maintained CDH but NK cell reconstitution was similar in both groups, suggesting that early reconstitution with T cells may play a role in preventing recovery of recipient cells after BMT. GVHD was also associated with maintenance of CDH, but the probability of relapse, survival, and disease-free survival was identical in patients with MC and CDH.

scholarly journals bcr Rearrangement without juxtaposition of c-abl in chronic myelocytic leukemia.

1985 ◽  
Vol 162 (6) ◽  
pp. 2175-2179 ◽  
Author(s):  
C R Bartram
Keyword(s):  
Blot Analysis ◽  
Southern Blot Analysis ◽  
Gene Rearrangement ◽  
Northern Blot Analysis ◽  
Philadelphia Chromosome ◽  
Leukemic Cells ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia ◽  
Genomic Recombination

Southern blot analysis detected a bcr gene rearrangement within leukemic cells of a Philadelphia chromosome-negative chronic myelocytic leukemia (CML) patient that led to transcription of a novel 7.3 kb bcr RNA species. Participation of the c-abl oncogene in this genomic recombination could be ruled out by in situ hybridization studies and Northern blot analysis.

Cytogenetic Studies of a Patient with Chronic Myelocytic Leukemia and his Nonleukemic Identical Twin

1970 ◽  
Vol 19 (1-2) ◽  
pp. 180-182 ◽  
Author(s):  
J. Bauke
Keyword(s):  
Blood Group ◽  
Pericardial Tamponade ◽  
Blood Group Antigens ◽  
Chronic Myelocytic Leukemia ◽  
Serum Factors ◽  
Hematological Disorders ◽  
Myelocytic Leukemia ◽  
Uncomplicated Pregnancy ◽  
History Of ◽  
Radiological Procedures

One of our patients with chronic myelocytic leukemia has a twin brother. The twins, 31 years old, show similar physical appearance. Both have blue eyes, brown hair and are right-handed. Anthropometric measurements and fingerprint patterns are matching. Blood group antigens, serum factors and PTC tasting are identical. Blood group antigens of the parents have also been determined. It has been calculated that the probability that the twins are MZ is higher than 99.8%.The twins were the result of an uncomplicated pregnancy. There are no other siblings. No family history of consanguinity, hematological disorders or cancer was given. They spent their childhood on a farm. Both have not been exposed to drugs, benzene or radiological procedures, apart from routine X-ray films of the chest.Our patient, who is still working on his farm, had been referred to a hospital in June 1959, because of a knife wound of the chest with a myocardial laceration and pericardial tamponade. White blood cell count at that time was 22300 with 3% basophils.In April 1962, the diagnosis of chronic myelocytic leukemia was established. Findings included hepatosplenomegaly, leukocytosis with a marked shift to the left, hyperplasia of granulopoiesis in the bone marrow and diminished leukocyte alkaline phosphatase activity. In the following years the patient was treated with cyclophosphamide, splenic irradiation and busulfan. Busulfan is continued at present at low doses.

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