Purification and characterization of granulocytic progenitor cells (CFU- C) from human peripheral blood using immunologic surface markers
Keyword(s):
The concentration of committed granulocytic progenitor cells (CFU-C) in functionally unique subpopulations of human peripheral blood mononuclear cells has been determined by the in vitro methyl-cellulose assay. Using immunoabsorbent column chromatography and rosette-depletion techniques, we have demonstrated that CFU-C, although not present in either purified T or B lymphocyte populations, are highly concentrated in the “null” cell population, which lacks sheep erythrocyte receptors and surface immunoglobulin. Further fractionation of this null subset has demonstrated that CFU-C do not bear complement receptors, but require the presence of peripheral blood mononuclear cell feeder layers for maximum proliferation.
1999 ◽
Vol 134
(1)
◽
pp. 49-55
◽
1992 ◽
Vol 54
(2)
◽
pp. 308-312
◽
2020 ◽
Vol 135
◽
pp. 110888
◽
1999 ◽
Vol 49
(4)
◽
pp. 411-416
◽
2010 ◽
Vol 14
◽
pp. S64