scholarly journals Serum erythropoietin (ESF) titers in polycythemia

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1171-1174 ◽  
Author(s):  
G de Klerk ◽  
PC Rosengarten ◽  
RJ Vet ◽  
R Goudsmit
Keyword(s):  
Polycythemia Vera ◽  
Clinical Importance ◽  
Hemoglobin Concentration ◽  
In Vitro Bioassay ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
The Mean ◽  
Serum Erythropoietin ◽  
Secondary Polycythemia

Abstract Serum ESF titers were measured in 42 polycythemic patients using the fetal mouse liver cell bioassay. ESF titers in patients with secondary polycythemia differed significantly from those in patients with polycythemia vera (p less than 0.0001). Among the 21 patients with secondary polycythemia, 1 patient had an ESF titer less than 10 mU/ml (the lower limit of sensitivity) and 20 had ESF titers that ranged between 11 and 112 mU/ml, with a mean titer of 56 mU/ml. Among the 21 patients with polycythemia vera, 13 patients had ESF titers less than 10 mU/ml and 8 had ESF titers ranging between 12 and 55 mU/ml, with a mean titer of 26 mU/ml. The mean hemoglobin concentration in the 8 patients with ESF titers greater than 10 mU/ml was significantly below that in the 13 polycythemia vera patients with ESF titers less than 10 mU/ml (p less than 0.03). If ESF titers less than 10 mU/ml had been indicative of polycythemia vera and ESF titers greater than 10 mU/ml had been indicative of secondary polycythemia in patients with hemoglobin concentrations greater than 17.7 g/dl, but not indicative of either condition in patients with hemoglobin concentrations less than 17.7 g/dl, 71.5% of the polycythemic patients in this study would have been diagnosed correctly, 9.5% incorrectly, and in the 19% the diagnosis would have remained uncertain. It was concluded that measurement of serum ESF titers using this in vitro bioassay can be of clinical importance in differentiating between polycythemia vera and secondary polycythemia.

scholarly journals Serum erythropoietin (ESF) titers in polycythemia

Blood ◽  
1981 ◽  
Vol 58 (6) ◽  
pp. 1171-1174 ◽  
Author(s):  
G de Klerk ◽  
PC Rosengarten ◽  
RJ Vet ◽  
R Goudsmit
Keyword(s):  
Polycythemia Vera ◽  
Clinical Importance ◽  
Hemoglobin Concentration ◽  
In Vitro Bioassay ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
The Mean ◽  
Serum Erythropoietin ◽  
Secondary Polycythemia

Serum ESF titers were measured in 42 polycythemic patients using the fetal mouse liver cell bioassay. ESF titers in patients with secondary polycythemia differed significantly from those in patients with polycythemia vera (p less than 0.0001). Among the 21 patients with secondary polycythemia, 1 patient had an ESF titer less than 10 mU/ml (the lower limit of sensitivity) and 20 had ESF titers that ranged between 11 and 112 mU/ml, with a mean titer of 56 mU/ml. Among the 21 patients with polycythemia vera, 13 patients had ESF titers less than 10 mU/ml and 8 had ESF titers ranging between 12 and 55 mU/ml, with a mean titer of 26 mU/ml. The mean hemoglobin concentration in the 8 patients with ESF titers greater than 10 mU/ml was significantly below that in the 13 polycythemia vera patients with ESF titers less than 10 mU/ml (p less than 0.03). If ESF titers less than 10 mU/ml had been indicative of polycythemia vera and ESF titers greater than 10 mU/ml had been indicative of secondary polycythemia in patients with hemoglobin concentrations greater than 17.7 g/dl, but not indicative of either condition in patients with hemoglobin concentrations less than 17.7 g/dl, 71.5% of the polycythemic patients in this study would have been diagnosed correctly, 9.5% incorrectly, and in the 19% the diagnosis would have remained uncertain. It was concluded that measurement of serum ESF titers using this in vitro bioassay can be of clinical importance in differentiating between polycythemia vera and secondary polycythemia.

scholarly journals Comparison of the hemagglutination inhibition assay kit for erythropoietin (ESF) with the fetal mouse liver cell bioassay in vitro

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Liver Cell ◽  
Hemagglutination Inhibition ◽  
Mouse Liver ◽  
Normal Human Serum ◽  
Fetal Mouse ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
Normal Human

The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.

scholarly journals Erythroid regulatory factors: correlation of detectable titers in vivo and in vitro

Blood ◽  
1978 ◽  
Vol 52 (6) ◽  
pp. 1238-1242
Author(s):  
CD Dunn ◽  
RD Lange
Keyword(s):  
Liver Cell ◽  
Mouse Liver ◽  
Fetal Mouse ◽  
Regulatory Factors ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver

Titers of erythroid regulatory factors (ERF), as measured in vivo with a standard erythrocythemic mouse technique, showed a highly significant correlation with those obtained in vitro with a fetal mouse liver cell (FMLC) assay. The results from the assay in vitro were somewhat higher than would be predicted from the assay in vivo. Nevertheless, the FMLC technique has been found to be a valid and useful technique particularly in the study of serum ERF titers.

Changes in the bioactivity to immunoreactivity ratio of circulating luteinizing hormone in impotent men treated with testosterone undecanoate

1989 ◽  
Vol 120 (3) ◽  
pp. 284-288 ◽  
Author(s):  
C. Carani ◽  
M. F. Celani ◽  
D. Zini ◽  
A. Baldini ◽  
L. Della Casa ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Serum Levels ◽  
Mean Value ◽  
Serum Concentrations ◽  
Placebo Administration ◽  
In Vitro Bioassay ◽  
Testosterone Undecanoate ◽  
The Mean ◽  
Mouse Leydig Cell

Abstract. Testosterone undecanoate was administered orally (80 mg twice daily) for 30 days to 10 impotent men with mild Leydig cell failure, age 28 to 42 years. Placebo was administered for 30 days both before and at the end of testosterone undecanoate therapy. Serum levels of bioactive LH, immunoreactive LH and testosterone were determined in basal conditions (day zero), 30 days after the first placebo administration, at the 15th and 30th day of testosterone undecanoate therapy, and at the end of the second treatment with placebo (90th day). Bioactive LH was measured by a sensitive and specific in vitro bioassay based on testosterone production by mechanically dispersed mouse Leydig cell preparations. Immunoreactive LH and testosterone were determined by a doubleantibody RIA technique. The results were compared with those obtained in 30 untreated normal young men. In the basal state, serum concentrations of immunoreactive LH were significantly higher in the patients (P< 0.02) than in control subjects, whereas testosterone levels were significantly lower (P< 0.001) in the impotent men. In contrast, bioactive LH levels and the bioactive LH to immunoreactive LH ratios were similar in the two groups. In the patients, at the 15th day of treatment with testosterone undecanoate, serum levels of testosterone and bioactive LH were significantly higher (P< 0.01) than basal values, whereas immunoreactive LH concentrations showed no significant changes. Consequently, the bioactive LH to immunoreactive LH ratios rose significantly (P< 0.01). At the 30th day of treatment with testosterone undecanoate, the mean value of bioactive LH and the mean bioactive LH to immunoreactive LH ratio were significantly higher (P< 0.01) in the patients than in control men, whereas the mean levels of testosterone and immunoreactive LH were similar in the two groups. Neither the first nor the second treatment with placebo changed the hormone values observed in basal conditions. The results support the experimental evidence that androgens may increase the bioactivity of circulating LH.

scholarly journals Comparison of the hemagglutination inhibition assay kit for erythropoietin (ESF) with the fetal mouse liver cell bioassay in vitro

Blood ◽  
1980 ◽  
Vol 55 (6) ◽  
pp. 955-959 ◽  
Author(s):  
G de Klerk ◽  
RJ Vet ◽  
PC Rosengarten ◽  
R Goudsmit
Keyword(s):  
Human Serum ◽  
Liver Cell ◽  
Hemagglutination Inhibition ◽  
Mouse Liver ◽  
Normal Human Serum ◽  
Fetal Mouse ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver ◽  
Normal Human

Abstract The commercially available hemagglutination inhibition (HAI) assay kit for erythropoietin (ESF) was compared with the fetal mouse liver cell (FMLC) bioassay. No correlation was obtained ESF levels determined by both methods in a variety of pathologic sera. The HAI kit showed a great batch variability. Significant immunoreactivity was found in those fractions of a normal human serum and a human urinary ESF preparation that were not active in the FMLC bioassay. A very poor recovery of immunoreactivity was found when the international reference preparation for erythropoietin (second IRPE) was added to a normal human serum.

Polyamines as an Inhibitor on Erythropoiesis of Hemodialysis Patients by In Vitro Bioassay Using the Fetal Mouse Liver Assay

2006 ◽  
Vol 10 (3) ◽  
pp. 267-272 ◽  
Author(s):  
Katsunori Yoshida ◽  
Tatsuo Yoneda ◽  
Syouki Kimura ◽  
Kiyohide Fujimoto ◽  
Eigoro Okajima ◽  
...  
Keyword(s):  
Mouse Liver ◽  
Hemodialysis Patients ◽  
In Vitro Bioassay ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

scholarly journals Serum erythropoietin concentration in chronic renal failure: relationship to degree of anemia and excretory renal function

Blood ◽  
1979 ◽  
Vol 54 (4) ◽  
pp. 877-884 ◽  
Author(s):  
HW Radtke ◽  
A Claussner ◽  
PM Erbes ◽  
EH Scheuermann ◽  
W Schoeppe ◽  
...  
Keyword(s):  
Renal Failure ◽  
Renal Function ◽  
Chronic Renal Failure ◽  
Healthy Subjects ◽  
Feedback Mechanism ◽  
Mouse Liver Cell ◽  
Short Period ◽  
Fetal Mouse Liver ◽  
Time Changes ◽  
Serum Erythropoietin

By use of the fetal mouse liver cell assay, serum erythropoietin (SEp) concentration was measured in 135 patients at various stages of chronic renal failure and in 59 healthy subjects. In patients with creatinine clearances (CCr) ranging from 2 to 40 ml/min/1.73 sq m, endocrine renal function was found to deteriorate in parallel to excretory renal function. The known negative correlation between SEp and hematocrit (Hct) was not apparent, probably because of the loss of renal mass accompanying progress of anemia and renal insufficiency. In contrast, in patients with minimal variation of residual excretory renal function, as in individual patients investigated repeatedly within a short period of time, changes of Hct were always accompanied by opposite changes of corresponding SEp concentrations. Thus, patients with chronic renal failure have a sustained regulatory feedback mechanism between Hct and SEp, which probably works at a lower level.

scholarly journals Conditions for an in vitro culture of murine mixed hematopoietic colonies and their putative cellular origin

Blood ◽  
1982 ◽  
Vol 60 (1) ◽  
pp. 99-107 ◽  
Author(s):  
BA Miller ◽  
DE Siedler ◽  
CD Dunn ◽  
AT Huang
Keyword(s):  
Gel Filtration ◽  
Colony Growth ◽  
Antigenic Determinants ◽  
Anion Exchange Column ◽  
Erythroid Progenitor ◽  
Cellular Origin ◽  
Mouse Liver Cell ◽  
Fetal Mouse Liver

Abstract The supernatant fluid of stimulated spleen cells (PHA-SCM) supported in vitro colony growth of murine marrow. In the absence of exogenous erythropoietin, it stimulated the growth of (1) myeloid colonies and (2) distinct mixed colonies containing erythroid cells, granulocytes, macrophages, and infrequently megakaryocytes in a setting structurally resembling biopsied marrow. The cells that form mixed colonies reside in a density range of 1.058–1.068 g/ml in a discontinuous albumin gradient. Active supernatant was produced by T cells in combination with a macrophage factor. DNA synthesis correlated with activity. PHA- SCM differed from erythropoietin (EPO) when chromatographed on lectin columns and did not contain EPO activity as demonstrated by the fetal mouse liver cell (FMLC) assay. The activity for mixed colony growth could be eluted from an anion exchange column with 0.07 M NaCl and eluted in a gel filtration column at a distance corresponding to a molecular weight of 39,000. Mixed colony-forming cells responsive to PHA-SCM were found to be Ia-H-2+. BFU-Es, CFU-Cs, and progenitors for myeloid colonies responsive to PHA-SCM were also H-2+ but showed significant sensitivity to anti-Ia antisera reflecting variable antigenic density. The mixed colony-forming cell appeared less differentiated than myeloid or erythroid progenitor cells examined, and its antigenic determinants are consistent with those observed for the pluripotent stem cell assayed in vivo (CFU-S).

Sign in / Sign up

Export Citation Format

Share Document