scholarly journals Analysis of natural killer cells in patients with aplastic anemia

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1349-1355 ◽  
Author(s):  
P Gascon ◽  
N Zoumbos ◽  
N Young
Keyword(s):  
Aplastic Anemia ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Normal Range ◽  
Granular Lymphocytes

Abstract We have analyzed natural killer (NK) cells in 43 patients with severe aplastic anemia, using cytotoxicity assays and microfluorometry with monoclonal antibodies, prior to and after treatment with antithymocyte globulin (ATG). Before treatment, natural killer cell activity (NKa) in both peripheral blood and bone marrow was markedly decreased in 76% of patients as compared with normal controls. Although we have measured low NKa in patients receiving large numbers of blood transfusions (means = 150 U of RBCs), six aplastic patients had low NKa in the absence of transfusions, and the average number of transfusions in the total population was low (means = 24). Purification of larger granular lymphocytes (LGLs) from peripheral blood of aplastic anemia patients failed to recover significant NKa. Most of these large granular lymphocytes showed few azurophilic granules. NKa was appropriately enhanced in these patients samples by exposure of mononuclear cells to either interleukin 2 (IL-2) or interferon (IFN). Analysis of peripheral blood phenotypic markers showed that cells bearing Leu 7 antigen were in the normal range in aplastic anemia (means = 12% +/- 2%; normal = 16% +/- 2%), but there was a deficiency of Leu 11+ cells (means = 8% +/- 2%; normal = 15% +/- 2%). The number of Leu 11+ cells was well correlated with NKa. In 13 of 22 patients treated with ATG, NKa returned to the normal range, and recovery of NKa was correlated to hematopoietic recovery. Our results suggest that deficient NKa is an intrinsic feature of aplastic anemia, and that the circulating cells in this disease are of the pre-NK cell stage.

scholarly journals Analysis of natural killer cells in patients with aplastic anemia

Blood ◽  
1986 ◽  
Vol 67 (5) ◽  
pp. 1349-1355
Author(s):  
P Gascon ◽  
N Zoumbos ◽  
N Young
Keyword(s):  
Aplastic Anemia ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Normal Range ◽  
Granular Lymphocytes

We have analyzed natural killer (NK) cells in 43 patients with severe aplastic anemia, using cytotoxicity assays and microfluorometry with monoclonal antibodies, prior to and after treatment with antithymocyte globulin (ATG). Before treatment, natural killer cell activity (NKa) in both peripheral blood and bone marrow was markedly decreased in 76% of patients as compared with normal controls. Although we have measured low NKa in patients receiving large numbers of blood transfusions (means = 150 U of RBCs), six aplastic patients had low NKa in the absence of transfusions, and the average number of transfusions in the total population was low (means = 24). Purification of larger granular lymphocytes (LGLs) from peripheral blood of aplastic anemia patients failed to recover significant NKa. Most of these large granular lymphocytes showed few azurophilic granules. NKa was appropriately enhanced in these patients samples by exposure of mononuclear cells to either interleukin 2 (IL-2) or interferon (IFN). Analysis of peripheral blood phenotypic markers showed that cells bearing Leu 7 antigen were in the normal range in aplastic anemia (means = 12% +/- 2%; normal = 16% +/- 2%), but there was a deficiency of Leu 11+ cells (means = 8% +/- 2%; normal = 15% +/- 2%). The number of Leu 11+ cells was well correlated with NKa. In 13 of 22 patients treated with ATG, NKa returned to the normal range, and recovery of NKa was correlated to hematopoietic recovery. Our results suggest that deficient NKa is an intrinsic feature of aplastic anemia, and that the circulating cells in this disease are of the pre-NK cell stage.

scholarly journals Human large granular lymphocytes and their relationship to natural killer cell activity in various disease states

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 288-295 ◽  
Author(s):  
G Gastl ◽  
D Niederwieser ◽  
C Marth ◽  
H Huber ◽  
D Egg ◽  
...  
Keyword(s):  
Natural Killer ◽  
Large Scale ◽  
Mononuclear Cells ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Human Tumor ◽  
Limiting Factors ◽  
Large Granular Lymphocytes ◽  
Granular Lymphocytes

Abstract This study investigated the numbers of large granular lymphocytes (LGL) and their relationship to natural killer (NK) function, as assessed by their capacity to lyse the human tumor target, K562. Peripheral blood mononuclear cells from 42 normal controls and from 171 patients suffering from various nonmalignant or malignant diseases were evaluated. Also studied were samples from a patient undergoing autologous bone marrow reconstitution following total body irradiation. Results suggested the existence of a close relationship between the numbers of LGL and the capacity to lyse K562 targets, further supporting the view that LGL are crucial effector cells mediating NK lysis. In certain diseases, such as malignant states, functional capacity was not simply determined by the numbers of LGL. Here preferential reduction of the capacity to lyse K562 targets was observed, indicating that additional limiting factors are involved in the determination of the cytotoxic potential. Based on the relationship between LGL and natural immune functions, as well as on the identification of leukemias affecting this cell type, we would recommend their evaluation on a large scale clinical basis.

scholarly journals Human large granular lymphocytes and their relationship to natural killer cell activity in various disease states

Blood ◽  
1984 ◽  
Vol 64 (1) ◽  
pp. 288-295 ◽  
Author(s):  
G Gastl ◽  
D Niederwieser ◽  
C Marth ◽  
H Huber ◽  
D Egg ◽  
...  
Keyword(s):  
Natural Killer ◽  
Large Scale ◽  
Mononuclear Cells ◽  
Natural Killer Cell Activity ◽  
Killer Cell ◽  
Cell Activity ◽  
Human Tumor ◽  
Limiting Factors ◽  
Large Granular Lymphocytes ◽  
Granular Lymphocytes

This study investigated the numbers of large granular lymphocytes (LGL) and their relationship to natural killer (NK) function, as assessed by their capacity to lyse the human tumor target, K562. Peripheral blood mononuclear cells from 42 normal controls and from 171 patients suffering from various nonmalignant or malignant diseases were evaluated. Also studied were samples from a patient undergoing autologous bone marrow reconstitution following total body irradiation. Results suggested the existence of a close relationship between the numbers of LGL and the capacity to lyse K562 targets, further supporting the view that LGL are crucial effector cells mediating NK lysis. In certain diseases, such as malignant states, functional capacity was not simply determined by the numbers of LGL. Here preferential reduction of the capacity to lyse K562 targets was observed, indicating that additional limiting factors are involved in the determination of the cytotoxic potential. Based on the relationship between LGL and natural immune functions, as well as on the identification of leukemias affecting this cell type, we would recommend their evaluation on a large scale clinical basis.

The effect of dietary control and carbohydrate supplementation on the immune and hormonal responses to rowing exercise

2006 ◽  
Vol 31 (5) ◽  
pp. 588-596 ◽  
Author(s):  
Christopher M. Sellar ◽  
Daniel G. Syrotuik ◽  
Catherine J. Field ◽  
Gordon J. Bell
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Peripheral Blood ◽  
Killer Cell ◽  
Interleukin 2 ◽  
Cell Activity ◽  
Dietary Control ◽  
Stress Hormone ◽  
Blood Leukocytes ◽  
Carbohydrate Supplementation

To determine the effect of carbohydrate supplementation on the immune and stress hormone responses to 1 h of strenuous rowing exercise, 22 male subjects were randomly assigned to a placebo (PLA, n = 11) or carbohydrate (CHO, n = 11) group. Subjects completed 3 d of modified dietary intake, a standardized pre-exercise meal, and consumed either a carbohydrate beverage (1 g·kg body mass–1) or a non-caloric placebo drink before, during, and after a 1 h rowing trial. Increases were observed in adrenocorticotrophic hormone, cortisol, blood leukocytes, neutrophils, and natural killer cell concentrations and activity, whereas the ability of peripheral blood monouclear cells (PBMCs) to respond (interleukin-2 (IL-2) production) to stimulation was reduced 5 min after exercise in both groups (p < 0.05). Lymphocytes were also elevated, but in the PLA group only (p < 0.05). One hour after exercise, blood leukocytes remained elevated owing to increased neutrophil concentrations, whereas a number of lymphocyte subsets (CD3+, CD3+/4+, CD3+/8+, CD20+, CD25+, CD4+/25+, CD8+/25+) and the ability of PBMCs to respond to stimulation (IL-2, interferon-γ (IFN-γ) production) were lower than resting values in both groups (p < 0.05). Carbohydrate supplementation to athletes in the post-prandial state undergoing a 1 h rowing trial resulted in attenuation of the post-exercise increase in peripheral blood lymphocyte concentration, but had little effect on the ability of PBMCs to produce cytokines following stimulation, natural killer cell activity, stress hormone concentrations, exercise performance, or self-reported incidence of illness during the 14 d period following the experimental trial.

Sign in / Sign up

Export Citation Format

Share Document