Human immunodeficiency virus infection of human bone marrow stromal fibroblasts

Abstract The human immunodeficiency virus (HIV) preferentially infects CD4 positive T cells and monocytes. Other human cell types have been reported to be infectable with HIV, including cells of mesenchymal origin. In this report, we show that both primary human bone marrow stromal fibroblasts and an immortalized human stromal fibroblast line are susceptible to HIV infection. These cells are capable of passing HIV to cells of lymphoid or myeloid lineage, and may thereby act as a reservoir of virus. This in vitro system may be a useful model for assessing the pathophysiology of hematopoietic dysfunction in AIDS patients.

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Human immunodeficiency virus infection of human bone marrow stromal fibroblasts

Blood ◽

10.1182/blood.v76.2.317.bloodjournal762317 ◽

1990 ◽

Vol 76 (2) ◽

pp. 317-322

Author(s):

DT Scadden ◽

M Zeira ◽

A Woon ◽

Z Wang ◽

L Schieve ◽

...

Keyword(s):

Bone Marrow ◽

Human Immunodeficiency Virus ◽

Cell Types ◽

Human Bone ◽

Human Bone Marrow ◽

Stromal Fibroblast ◽

Stromal Fibroblasts ◽

Fibroblast Line ◽

Immunodeficiency Virus

The human immunodeficiency virus (HIV) preferentially infects CD4 positive T cells and monocytes. Other human cell types have been reported to be infectable with HIV, including cells of mesenchymal origin. In this report, we show that both primary human bone marrow stromal fibroblasts and an immortalized human stromal fibroblast line are susceptible to HIV infection. These cells are capable of passing HIV to cells of lymphoid or myeloid lineage, and may thereby act as a reservoir of virus. This in vitro system may be a useful model for assessing the pathophysiology of hematopoietic dysfunction in AIDS patients.

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Human immunodeficiency virus infection of eosinophils in human bone marrow cultures.

Journal of Experimental Medicine ◽

10.1084/jem.174.6.1661 ◽

1991 ◽

Vol 174 (6) ◽

pp. 1661-1664 ◽

Cited By ~ 29

Author(s):

A R Freedman ◽

F M Gibson ◽

S C Fleming ◽

C J Spry ◽

G E Griffin

Keyword(s):

Bone Marrow ◽

Human Immunodeficiency Virus ◽

Reverse Transcriptase Activity ◽

Human Bone ◽

Human Bone Marrow ◽

Rapid Decline ◽

Immunodeficiency Virus ◽

Hiv 1

Normal human bone marrow, cultured in vitro with interleukin 5 to promote eosinophil production and maturation, was inoculated with cell-free isolates of human immunodeficiency virus type 1 (HIV-1). CD4 expression by eosinophil precursors, determined by immunocytochemistry, was found to be greatest early in their maturation with a rapid decline after 28 d in culture. Productive HIV infection of eosinophil precursors was detected 14 d after inoculation, by a combination of immunostaining for HIV-1 p24 and gp41/160 and in situ hybridization for viral RNA, together with assay of culture supernatants for p24 antigen and reverse transcriptase activity. Thus, eosinophils are susceptible to productive HIV-1 infection in vitro and may be an important reservoir for the virus in vivo.

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In vitro suppression of normal human bone marrow progenitor cells by human immunodeficiency virus.

Journal of Virology ◽

10.1128/jvi.65.4.1765-1769.1991 ◽

1991 ◽

Vol 65 (4) ◽

pp. 1765-1769 ◽

Cited By ~ 71

Author(s):

H N Steinberg ◽

C S Crumpacker ◽

P A Chatis

Keyword(s):

Bone Marrow ◽

Human Immunodeficiency Virus ◽

Progenitor Cells ◽

Human Bone ◽

Human Bone Marrow ◽

Bone Marrow Progenitor ◽

Immunodeficiency Virus ◽

Normal Human ◽

Normal Human Bone Marrow

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Human eosinophil hematopoiesis studied in vitro by means of murine eosinophil differentiation factor (IL5): production of functionally active eosinophils from normal human bone marrow

Blood ◽

10.1182/blood.v71.3.646.bloodjournal713646 ◽

1988 ◽

Vol 71 (3) ◽

pp. 646-651

Author(s):

EJ Clutterbuck ◽

CJ Sanderson

Keyword(s):

Bone Marrow ◽

Cell Types ◽

Human Bone ◽

Human Bone Marrow ◽

Electron Microscopic ◽

Human Eosinophil ◽

Interleukin 5 ◽

Differentiation Factor ◽

Microscopic Features

The production of human eosinophils in vitro from normal bone marrow by using murine eosinophil differentiation factor (mEDF/interleukin 5) is described. Eosinophil production was selective and first detectable after 14 days and reached a peak between 21 and 35 days when they were the predominant cell type (41% to 89%). Until day 14, all the eosinophils were typical myelocytes, developing thereafter into metamyelocytes and mature cells. All cell types had characteristic light- and electron-microscopic features, apart from the absence of granules with crystalline cores. The eosinophils produced were readily recovered, and both immature myelocytes and mature cells were functionally active in an antibody-dependent, cell-mediated cytotoxicity assay. mEDF added into the assay enhanced the cytotoxicity but to a lower degree than previously reported for peripheral blood eosinophils, which suggests that they may be partially activated. The possibility that eosinophils could be deactivated was tested by removing mEDF from the culture medium. The eosinophils retained viability and functional activity, however, and showed no increased ability to be activated by mEDF for up to six days after removing the mEDF. The liquid culture of human bone marrow was shown to be an alternative assay for eosinophil differentiation factors to colony formation.

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Human eosinophil hematopoiesis studied in vitro by means of murine eosinophil differentiation factor (IL5): production of functionally active eosinophils from normal human bone marrow

Blood ◽

10.1182/blood.v71.3.646.646 ◽

1988 ◽

Vol 71 (3) ◽

pp. 646-651 ◽

Cited By ~ 75

Author(s):

EJ Clutterbuck ◽

CJ Sanderson

Keyword(s):

Bone Marrow ◽

Cell Types ◽

Human Bone ◽

Human Bone Marrow ◽

Electron Microscopic ◽

Human Eosinophil ◽

Interleukin 5 ◽

Differentiation Factor ◽

Microscopic Features

Abstract The production of human eosinophils in vitro from normal bone marrow by using murine eosinophil differentiation factor (mEDF/interleukin 5) is described. Eosinophil production was selective and first detectable after 14 days and reached a peak between 21 and 35 days when they were the predominant cell type (41% to 89%). Until day 14, all the eosinophils were typical myelocytes, developing thereafter into metamyelocytes and mature cells. All cell types had characteristic light- and electron-microscopic features, apart from the absence of granules with crystalline cores. The eosinophils produced were readily recovered, and both immature myelocytes and mature cells were functionally active in an antibody-dependent, cell-mediated cytotoxicity assay. mEDF added into the assay enhanced the cytotoxicity but to a lower degree than previously reported for peripheral blood eosinophils, which suggests that they may be partially activated. The possibility that eosinophils could be deactivated was tested by removing mEDF from the culture medium. The eosinophils retained viability and functional activity, however, and showed no increased ability to be activated by mEDF for up to six days after removing the mEDF. The liquid culture of human bone marrow was shown to be an alternative assay for eosinophil differentiation factors to colony formation.

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