Genetic and structural characterization of an amino acid dimorphism in glycoprotein Ib alpha involved in platelet transfusion refractoriness

Abstract The platelet-specific alloantigen, Siba, located within the alpha- subunit of the glycoprotein (GP) Ib-IX membrane receptor, has been found to be involved in the pathogenesis of platelet transfusion refractoriness. We have identified the existence of a naturally occurring threonine/methionine dimorphism at position 145 of the GPIb alpha sequence, and determined that the Siba antigen corresponds to the molecule containing methionine145. The diallelic codons can be detected by restriction enzyme analysis of amplified genomic DNA fragments from the GPIb alpha gene. Evaluation of 61 healthy blood donors showed that the allele frequencies are 89% and 11% for the threonine145 and methionine145 codons, respectively. A positive correlation exists between platelet reactivity with the anti-Siba antibody and the presence of a methionine145-encoding allele. Moreover, recombinant expression of two soluble GPIb alpha fragments differing only at residue 145, provided definitive evidence that the human anti-Siba antibody reacts only with the molecule containing methionine145. These results explain the structural basis of the Siba human alloantigen system and define screening methodologies useful in transfusion medicine to match donor and recipient platelets accordingly.

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Genetic and structural characterization of an amino acid dimorphism in glycoprotein Ib alpha involved in platelet transfusion refractoriness

Blood ◽

10.1182/blood.v79.11.3086.bloodjournal79113086 ◽

1992 ◽

Vol 79 (11) ◽

pp. 3086-3090 ◽

Cited By ~ 2

Author(s):

M Murata ◽

K Furihata ◽

F Ishida ◽

SR Russell ◽

J Ware ◽

...

Keyword(s):

Platelet Transfusion ◽

Platelet Reactivity ◽

Recombinant Expression ◽

Structural Basis ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Definitive Evidence ◽

Naturally Occurring ◽

Alpha Gene

The platelet-specific alloantigen, Siba, located within the alpha- subunit of the glycoprotein (GP) Ib-IX membrane receptor, has been found to be involved in the pathogenesis of platelet transfusion refractoriness. We have identified the existence of a naturally occurring threonine/methionine dimorphism at position 145 of the GPIb alpha sequence, and determined that the Siba antigen corresponds to the molecule containing methionine145. The diallelic codons can be detected by restriction enzyme analysis of amplified genomic DNA fragments from the GPIb alpha gene. Evaluation of 61 healthy blood donors showed that the allele frequencies are 89% and 11% for the threonine145 and methionine145 codons, respectively. A positive correlation exists between platelet reactivity with the anti-Siba antibody and the presence of a methionine145-encoding allele. Moreover, recombinant expression of two soluble GPIb alpha fragments differing only at residue 145, provided definitive evidence that the human anti-Siba antibody reacts only with the molecule containing methionine145. These results explain the structural basis of the Siba human alloantigen system and define screening methodologies useful in transfusion medicine to match donor and recipient platelets accordingly.

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Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA

Annals of Tropical Medicine and Parasitology ◽

10.1080/00034983.1992.11812659 ◽

1992 ◽

Vol 86 (3) ◽

pp. 231-237 ◽

Cited By ~ 4

Author(s):

T. Mimori ◽

M. Maldonado ◽

M. Samudio ◽

A. Rojas De Arias ◽

R. Moreno ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Restriction Enzyme ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Kinetoplast Dna

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Characterization of two BHV-4 strains isolated in the Czech Republic

Veterinární Medicína ◽

10.17221/18/2010-vetmed ◽

2010 ◽

Vol 55 (No. 3) ◽

pp. 106-112 ◽

Cited By ~ 1

Author(s):

V. Fichtelova ◽

K. Kovarcik

Keyword(s):

Reference Strain ◽

Fragment Size ◽

Blot Hybridization ◽

Size Variation ◽

Southern Blot Hybridization ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

The Czech Republic ◽

Herpesvirus 4

This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with BamHI, EcoRI and HindIII in restriction enzyme analysis and the fragments were identified by co-migration with the restriction profile of the reference strain Movar 33/63 cleaved with the appropriate endonuclease. Typical profiles with polyrepetitive DNA (prDNA) fragments were detected. In order to localize the size variation within the obtained digestion fragments, Southern blot hybridization was performed. Differences between the isolates CH, Ni were localized in both the prDNAs and the unique central part of the genome and were restricted to fragment size variation.

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Characterization of the DNA of Canine Adenovirus by Restriction Enzyme Analysis

Intervirology ◽

10.1159/000149563 ◽

1985 ◽

Vol 23 (1) ◽

pp. 23-28 ◽

Cited By ~ 6

Author(s):

Gholamreza Darai ◽

Angela Rösen ◽

Hajo Delius ◽

Rolf M. Flügel

Keyword(s):

Restriction Enzyme ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis

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Characterization of a mutant polyoma that expresses in F9 embryonal carcinoma cells: Morphology, tumorigenicity, and restriction enzyme analysis

Experimental and Molecular Pathology ◽

10.1016/0014-4800(87)90007-4 ◽

1987 ◽

Vol 47 (1) ◽

pp. 59-68

Author(s):

John M. Lehman ◽

Jill McMahon ◽

Karen Hales ◽

Katrina Trevor

Keyword(s):

Restriction Enzyme ◽

Embryonal Carcinoma ◽

Carcinoma Cells ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Embryonal Carcinoma Cells ◽

F9 Embryonal Carcinoma Cells

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Molecular and Pathotypic Characterization of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Indian Chickens

10.21203/rs.3.rs-64286/v1 ◽

2020 ◽

Author(s):

Dasharath B. Shinde ◽

Anil L Thormoth ◽

Santosh S. Koratkar ◽

Neeti Sharma ◽

Ashok Rajguru ◽

...

Keyword(s):

Inclusion Body ◽

Broiler Chickens ◽

Emerging Diseases ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Hydropericardium Syndrome ◽

Inclusion Body Hepatitis ◽

Infective Dose ◽

Pathogenic Analysis

Abstract Inclusion body hepatitis (IBH) is a disease of chickens characterized by acute mortality with severe anemia caused by fowl adenoviruses (FAdV). IBH is one of the most important re-emerging diseases world-wide. In India earlier IBH/ Hydropericardium Syndrome (HPS) outbreaks were mainly due to FAdV) type 4; however, in recent outbreaks, other serotypes are involved, despite regular vaccination in breeders and young chicks. The aim of the present study was isolation and molecular characterization of FAdV associated with IBH in India. A total of 193 liver samples of IBH suspected broiler chickens were collected from different regions of India. Liver samples were initially screened by histopathology and further, a total of 127 samples which microscopically showed basophilic, intra-nuclear inclusion bodies were processed for FAdV detection. A total of 69 samples were found to be positive for Hexon gene in PCR, of which 28 were sequenced. These samples showed more than 94% sequence homology with FAdV2 and 97% with FAdV11, which was confirmed by Restriction Enzyme Analysis (REA). Tissue Culture Infective Dose 50 (TCID50) and Egg Infective Dose50 (EID50) titer of isolated FAdV was found to be 106.5/ml. Pathogenicity study in SPF chicks showed 100% mortality up to Post Infection Day (PID)-6. These findings conclude that the prevalence of FAdV-11 is currently causing IBH outbreaks India. However, further genetic and pathogenic analysis of FAdV is required, which would provide useful information for the development of the efficient commercial IBH vaccine.

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Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR-restriction enzyme analysis, 16S rRNA gene sequencing, and DT1-DT6 PCR.

Journal of Clinical Microbiology ◽

10.1128/jcm.35.11.2767-2772.1997 ◽

1997 ◽

Vol 35 (11) ◽

pp. 2767-2772 ◽

Cited By ~ 17

Author(s):

A Devallois ◽

M Picardeau ◽

C N Paramasivan ◽

V Vincent ◽

N Rastogi

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Mycobacterium Avium Complex ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Rrna Gene ◽

Rrna Gene Sequencing

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Further characterization of 41 isolates of adenovirus types 19/37 by serum neutralization and DNA restriction enzyme analysis

Journal of Hygiene ◽

10.1017/s0022172400065463 ◽

1986 ◽

Vol 97 (2) ◽

pp. 377-383 ◽

Cited By ~ 7

Author(s):

Zong-Da Meng ◽

Margery L. Kennett ◽

Suzanne M. Rodger ◽

Kaye E. Dickson ◽

Bruce N. Anderson ◽

...

Keyword(s):

Restriction Analysis ◽

Haemagglutination Inhibition ◽

Adenovirus Type ◽

Cross Reactivity ◽

High Titre ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Serum Neutralization ◽

Dna Restriction

SummaryForty-one strains of adenovirus type 19/37 (Ad19/37) mainly isolatedI from patients with keratoconjunetivitis or conjunctive between 1974 and 1984 were re-evaluated by serum neutralization (SN), haemagglutination inhibition (HI) and DNA restriction analysis. Of 19 isolates which were neutralized to high titre by antiserum prepared against prototype Ad19, 5 showed cross-reactivity with 32–64 units of Ad37 antiserum, while of 22 strains neutralizedto high titre by Ad37 antiserum, 3 showed eross-reaetivity with 32 units of Ad19 antiserum By DNA restriction analysis, all Ad19 isolates were identieal to each other and to Ad19A virus. Using endonuclease Bgl 1, three variants were observed among the Ad37 isolates.

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Incidence of antibiotic resistance and characterization of plasmids in Campylobacter jejuni strains isolated from clinical sources in Alberta, Canada

Canadian Journal of Microbiology ◽

10.1139/m86-006 ◽

1986 ◽

Vol 32 (1) ◽

pp. 28-32 ◽

Cited By ~ 15

Author(s):

Diane E. Taylor ◽

N. Chang ◽

R. S. Garner ◽

R. Sherburne ◽

L. Mueller

Keyword(s):

Antibiotic Resistance ◽

Campylobacter Jejuni ◽

Tetracycline Resistance ◽

Additional Restriction ◽

Enzyme Analysis ◽

Restriction Enzyme Analysis ◽

Antibiotic Susceptibilities ◽

Restriction Sites ◽

High Level

Antibiotic susceptibilities of 382 strains of Campylobacter jejuni isolated in Alberta, Canada, in 1980 and 1981 were determined. Although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. Tetracycline resistance was noted in 6.8% of strains in 1980 and in 8.6% in 1981. Moreover, resistance to high-level tetracycline (32–128 μg/mL) was always mediated by a plasmid of 45–50 kilobases. Three transmissible plasmids from the Alberta strains were compared with the prototype plasmid pMAK175 by restriction enzyme analysis and some minor differences in restriction sites were noted between pMAK175 and pUA183. The two other plasmids pUA142 and pUA143 were 4 kilobases larger than pMAK175 and contained additional restriction sites. However, in all plasmids examined, the HincII and AccI fragments where the tetracycline-resistance determinant was located were shown to be conserved.

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