scholarly journals Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle

2013 ◽  
Vol 44 (1) ◽  
pp. 48 ◽  
Author(s):  
Hui-Chen Guo ◽  
Shi-Qi Sun ◽  
Ye Jin ◽  
Shun-Li Yang ◽  
Yan-Quan Wei ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Fusion Protein ◽  
Disease Virus ◽  
Guinea Pigs ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Virus Like Particles ◽  
Mouth Disease Virus ◽  
Sumo Fusion

Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

Intervirology ◽  
2015 ◽  
Vol 58 (3) ◽  
pp. 190-196 ◽  
Author(s):  
Farahnaz Motamedi-Sedeh ◽  
Hoorieh Soleimanjahi ◽  
Amir Reza Jalilian ◽  
Homayoon Mahravani ◽  
Kamalodin Shafaee ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Guinea Pigs ◽  
Gamma Ray ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Economic Losses ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Fmdv Type

Objectives: Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals and is the most damaging disease of livestock worldwide, leading to great economic losses. The aim of this research was the inactivation of FMDV type O/IRN/1/2007 to produce a gamma ray-irradiated (GRI) vaccine in order to immunize mice and guinea pigs. Methods: In this research, the Iranian isolated FMDV type O/IRN/1/2007 was irradiated by gamma ray to prepare an inactivated whole virus antigen and formulated as a GRI vaccine with unaltered antigenic characteristics. Immune responses against this vaccine were evaluated on mice and guinea pigs. Results: The comparison of the immune responses between the GRI vaccine and conventional vaccine did not show any significant difference in neutralizing antibody titer, memory spleen T lymphocytes or IFN-γ, IL-4, IL-2 and IL-10 concentrations (p > 0.05). In contrast, there were significant differences in all of the evaluated immune factors between the two vaccinated groups of mice and negative control mice (p < 0.05). The protective dose 50 for the conventional and GRI vaccines obtained were 6.28 and 7.07, respectively, which indicated the high potency of both vaccines. Conclusion: GRI vaccine is suitable for both routine vaccination and control of FMDV in emergency outbreaks.

CpG DNA enhances the immune responses elicited by the DNA vaccine against foot-and-mouth disease virus in guinea pigs

2001 ◽  
Vol 46 (16) ◽  
pp. 1376-1379 ◽  
Author(s):  
Guangjin Li ◽  
Yingjie Li ◽  
Weiyao Yan ◽  
Quanxin Xu ◽  
Yongqing Wu ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Dna Vaccine ◽  
Guinea Pigs ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Cpg Dna ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Sreerupa Challa ◽  
Steven M. Szczepanek ◽  
Debra Rood ◽  
Roger W. Barrette ◽  
Lawrence K. Silbart
Keyword(s):  
Immune Responses ◽  
Disease Virus ◽  
Mucosal Immunity ◽  
Guinea Pigs ◽  
Specific Antigen ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Mucosal Immune Responses

Peptides corresponding to the foot-and-mouth disease virus VP1 G-H loop are capable of inducing neutralizing antibodies in some species but are considered relatively poor immunogens, especially at mucosal surfaces. However, intranasal administration of antigens along with the appropriate delivery vehicle/adjuvant has been shown to induce mucosal immune responses, and bacterial enterotoxins have long been known to be effective in this regard. In the current study, two different carrier/adjuvant approaches were used to augment mucosal immunity to the FMDV O1BFS G-H loop epitope, in which the G-H loop was genetically coupled to theE. coliLT-B subunit and coexpressed with the LTA2 fragment (LTA2B-GH), or the nontoxic pseudomonas exotoxin A (ntPE) was fused to LTA2B-GH at LT-A2 to enhance receptor targeting. Only guinea pigs that were inoculated intranasally with ntPE-LTA2B-GH and LTA2B-GH induced significant anti-G-H loop IgA antibodies in nasal washes at weeks 4 and 6 when compared to ovalbumin or G-H loop immunized animals. These were also the only groups that exhibited G-H loop-specific antigen-secreting cells in the nasal mucosa. These data demonstrate that fusion of nonreplicating antigens to LTA2B and ntPE-LTA2B has the potential to be used as carriers/adjuvants to induce mucosal immune responses against infectious diseases.

scholarly journals Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

2004 ◽  
Vol 36 (9) ◽  
pp. 589-596 ◽  
Author(s):  
Jian-Zhong Yi ◽  
Ming-Qiu Liu ◽  
Cai-Zhu Zhu ◽  
Qiang Zhang ◽  
Zu-Tian Sheng ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Disease Virus ◽  
Tandem Repeat ◽  
Guinea Pigs ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype A ◽  
Serotype O ◽  
Mouth Disease Virus ◽  
Virus Serotype

Abstract In this study, two DNA fragments encoding amino acid (141– 160)-(21–40)-(141–160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138–160)-(21–40)-(138–160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 μg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

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