scholarly journals Characterization of adipose-derived stromal/stem cells from the twitcher mouse model of krabbe disease

2013 ◽  
Vol 14 (1) ◽  
pp. 20 ◽  
Author(s):  
Xiujuan Zhang ◽  
Julie A Semon ◽  
Shijia Zhang ◽  
Amy L Strong ◽  
Brittni A Scruggs ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Krabbe Disease ◽  
Twitcher Mouse ◽  
Stromal Stem Cells

scholarly journals Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 750
Author(s):  
Pasquale Marrazzo ◽  
Valeria Pizzuti ◽  
Silvia Zia ◽  
Azzurra Sargenti ◽  
Daniele Gazzola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Defense Mechanisms ◽  
Live Cells ◽  
Label Free ◽  
Bacterial Diseases ◽  
Prospective Application ◽  
Therapy Strategies ◽  
Stromal Stem Cells ◽  
Different Sources

Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.

840 DIFFERENTIAL ISOLATION AND SURFACE MARKER CHARACTERIZATION OF EPITHELIAL AND STROMAL STEM CELLS IN BENIGN PROSTATE TISSUE

2011 ◽  
Vol 10 (2) ◽  
pp. 266
Author(s):  
N. Neli ◽  
J. Hennenlotter ◽  
C. Schwentner ◽  
T. Todenhoefer ◽  
S. Grimm ◽  
...  
Keyword(s):  
Stem Cells ◽  
Surface Marker ◽  
Prostate Tissue ◽  
Stromal Stem Cells ◽  
Benign Prostate

scholarly journals Isolation, Characterization, Differentiation and Immunomodulatory Capacity of Mesenchymal Stromal/Stem Cells from Human Perirenal Adipose Tissue

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1346 ◽  
Author(s):  
Baer ◽  
Koch ◽  
Hickmann ◽  
Schubert ◽  
Cinatl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Epithelial Cells ◽  
Lipoteichoic Acid ◽  
Differentiation Potential ◽  
Perivascular Niche ◽  
Perirenal Adipose Tissue ◽  
Multipotent Cells ◽  
Stromal Stem Cells

Mesenchymal stromal/stem cells (MSCs) are immature multipotent cells, which represent a rare population in the perivascular niche within nearly all tissues. The most abundant source to isolate MSCs is adipose tissue. Currently, perirenal adipose tissue is rarely described as the source of MSCs. MSCs were isolated from perirenal adipose tissue (prASCs) from patients undergoing tumor nephrectomies, cultured and characterized by flow cytometry and their differentiation potential into adipocytes, chondrocytes, osteoblasts and epithelial cells. Furthermore, prASCs were stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA) or a mixture of cytokines (cytomix). In addition, prASC susceptibility to human cytomegalovirus (HCMV) was investigated. The expression of inflammatory readouts was estimated by qPCR and immunoassay. HCMV infection was analyzed by qPCR and immunostaining. Characterization of cultured prASCs shows the cells meet the criteria of MSCs and prASCs can undergo trilineage differentiation. Cultured prASCs can be induced to differentiate into epithelial cells, shown by cytokeratin 18 expression. Stimulation of prASCs with LPS or cytomix suggests the cells are capable of initiating an inflammation-like response upon stimulation with LPS or cytokines, whereas, LTA did not induce a significant effect on the readouts (ICAM-1, IL-6, TNFα, MCP-1 mRNA and IL-6 protein). HCMV broadly infects prASCs, showing a viral load dependent cytopathological effect (CPE). Our current study summarizes the isolation and culture of prASCs, clearly characterizes the cells, and demonstrates their immunomodulatory potential and high permissiveness for HCMV.

Comprehensive Phenotypic Characterization of Human Adipose-Derived Stromal/Stem Cells and Their Subsets by a High Throughput Technology

2013 ◽  
Vol 22 (2) ◽  
pp. 330-339 ◽  
Author(s):  
Patrick C. Baer ◽  
Selim Kuçi ◽  
Michael Krause ◽  
Zyrafete Kuçi ◽  
Stefan Zielen ◽  
...  
Keyword(s):  
Stem Cells ◽  
High Throughput ◽  
Phenotypic Characterization ◽  
High Throughput Technology ◽  
Stromal Stem Cells

Antiobesity Effects of Adipose‐Derived Stromal/Stem Cells in a Naturally Aged Mouse Model

Obesity ◽  
2020 ◽  
Vol 29 (1) ◽  
pp. 133-142
Author(s):  
Qiong Wu ◽  
Shuangli He ◽  
Yu Zhu ◽  
Shiming Pu ◽  
Zuping Zhou
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Aged Mouse ◽  
Stromal Stem Cells

scholarly journals Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Valentina Cappello ◽  
Laura Marchetti ◽  
Paola Parlanti ◽  
Silvia Landi ◽  
Ilaria Tonazzini ◽  
...  
Keyword(s):  
Mouse Model ◽  
Motor System ◽  
Krabbe Disease ◽  
Ultrastructural Characterization

scholarly journals Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation

2013 ◽  
Vol 4 (3) ◽  
pp. 75 ◽  
Author(s):  
Khurram Hashmani ◽  
Matthew Branch ◽  
Laura Sidney ◽  
Permesh Dhillon ◽  
Megha Verma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stromal Stem Cells

Use of AAVrh10-GALC to Treat the Twitcher Mouse Model of Krabbe Disease

2012 ◽  
Vol 105 (2) ◽  
pp. S64
Author(s):  
David Wenger ◽  
Mohammad Rafi ◽  
Paola Luzi ◽  
Han Zhi Rao
Keyword(s):  
Mouse Model ◽  
Krabbe Disease ◽  
Twitcher Mouse

scholarly journals Author response: MHC-compatible bone marrow stromal/stem cells trigger fibrosis by activating host T cells in a scleroderma mouse model

2015 ◽  
Author(s):  
Yoko Ogawa ◽  
Satoru Morikawa ◽  
Hideyuki Okano ◽  
Yo Mabuchi ◽  
Sadafumi Suzuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
T Cells ◽  
Mouse Model ◽  
Author Response ◽  
Stromal Stem Cells
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