Toona sinensisRoem is a popular leafy vegetable in Chinese cuisine and is also used as a traditional Chinese medicine. In this study, leaf samples were collected from the same plant on two development stages and then used for high-throughput Illumina RNA-sequencing (RNA-Seq). 125,884 transcripts and 54,628 unigenes were obtained throughde novoassembly. A total of 25,570 could be annotated with known biological functions, which indicated that theT. sinensisleaves and shoots were undergoing multiple developmental processes especially for active metabolic processes. Analysis of differentially expressed unigenes between the two libraries showed that the lysine biosynthesis was an enriched KEGG pathway, and candidate genes involved in the lysine biosynthesis pathway inT. sinensisleaves and shoots were identified. Our results provide a primary analysis of the gene expression files ofT. sinensisleaf and shoot on different development stages and afford a valuable resource for genetic and genomic research on plant lysine biosynthesis.
De novo assembly of the desert tree Haloxylon ammodendron (C. A. Mey.) based on RNA-Seq data provides insight into drought response, gene discovery and marker identification