scholarly journals Fragmented mitochondrial genomes are present in both major clades of the blood-sucking lice (suborder Anoplura): evidence from two Hoplopleura rodent lice (family Hoplopleuridae)

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 751 ◽  
Author(s):  
Wen-Ge Dong ◽  
Simon Song ◽  
Xian-Guo Guo ◽  
Dao-Chao Jin ◽  
Qianqian Yang ◽  
...  
Keyword(s):  
Mitochondrial Genomes ◽  
Blood Sucking ◽  
Sucking Lice

scholarly journals New Records of Bartonella Spp. And Rickettsia Spp. In Lice Collected From Small Rodents

2020 ◽  
Author(s):  
Asta Aleksandravičienė ◽  
Algimantas Paulauskas ◽  
Michal Stanko ◽  
Jana Fričová ◽  
Jana Radzijevskaja
Keyword(s):  
Sequence Analysis ◽  
New Records ◽  
Myodes Glareolus ◽  
Molecular Characterisation ◽  
Infectious Agents ◽  
Human Pathogens ◽  
Small Rodents ◽  
Blood Sucking ◽  
Sucking Lice ◽  
Pcr Targeting

Abstract Background: Lice are blood-sucking insects that are of medical and veterinary significance as parasites and vectors for various infectious agents. More than half of described blood-sucking lice species are found on rodents. Rodents are important hosts of several Bartonella and Rickettsia species and some of these pathogens are characterised as human pathogens in Europe. Rodent ectoparasites, such as fleas and ticks, are important vectors of Bartonella spp. and Rickettsia spp., but knowledge about the presence of these bacteria in lice is limited. The aim of this study was to determine the prevalence of Bartonella and Rickettsia bacteria in lice collected from rodents in Slovakia.Methods: The ectoparasites were collected from small rodents captured from 2010 to 2015 at four different sites in eastern Slovakia. The presence of Bartonella and Rickettsia pathogens in lice samples was screened by real-time PCR, targeting ssrA and gltA genes respectively. The molecular characterisation of the Bartonella strains was based on sequence analysis of partial rpoB and ITS genes, and of the Rickettsia species on sequence analysis of the gltA gene. Results: A total of 1074 lice of seven species were collected from six rodent species in Slovakia from 2010 to 2015. Bartonella DNA was detected in three species of lice Hoplopleura affinis (collected from Apodemus agrarius, A. flavicollis and Myodes glareolus), Polyplax serrata (from A. agrarius) and Hoplopleura sp. (from A. flavicollis). Sequence analysis revealed that the Bartonella strains belonged to the B. coopersplainsensis, B. tribocorum and B. taylorii genogroups. Rickettsia DNR was detected in H. affinis and P. serrata lice collected from A. agrarius. Sequence analysis revealed the presence of two Rickettsia species: R. helvetica and Rickettsia sp.Conclusions: To the best of the authors’ knowledge, this is the first report on the occurrence and diversity of Bartonella spp. and Rickettsia spp. in lice collected from small rodents in Europe. This study is also the first to detect B. coopersplainsensis in Slovakia.

scholarly journals The Incubation Period of the Eggs of Haematopinus asini

Parasitology ◽  
1919 ◽  
Vol 11 (3-4) ◽  
pp. 388-392 ◽  
Author(s):  
A. Bacot ◽  
L. Linzell
Keyword(s):  
Incubation Period ◽  
Blood Sucking ◽  
Sucking Lice

Three kinds of lice are found on horses, two belonging to the Mallophaga or scale-eating lice and one to the Siphunculata or blood-sucking lice.

First molecular identification of Mycoplasma ovis and ‘Candidatus M. haemoovis’ from goat, with lack of haemoplasma PCR-positivity in lice

2012 ◽  
Vol 60 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Sándor Hornok ◽  
István Hajtós ◽  
Marina Meli ◽  
Imre Farkas ◽  
Enikő Gönczi ◽  
...  
Keyword(s):  
Inclusion Bodies ◽  
Bacterial Load ◽  
The Other ◽  
Simultaneous Presence ◽  
Cloning And Sequencing ◽  
Blood Samples ◽  
Blood Sucking ◽  
Blood Smears ◽  
Molecular Investigation ◽  
Sucking Lice

In order to investigate haemotropic Mycoplasma (formerly Eperythrozoon) infection of goats, blood samples and blood-sucking lice (Linognathus stenopsis) were collected in two goat herds. DNA was extracted from 20 blood samples and from 49 lice allocated to six pools according to host individuals. Haemoplasma infection was detected in four goats by real-time PCR. From the sample with the highest bacterial load the simultaneous presence of M. ovis and ‘Candidatus M. haemoovis’ was demonstrated by cloning and sequencing. Louse pools were haemoplasma negative, including those from bacteraemic animals. However, not only were Anaplasma inclusion bodies seen in blood smears from goats, but relevant PCR-positivity was also detected among lice. This is the first report of a molecular investigation on caprine haemoplasmas, including analysis of their bloodsucking lice. In summary, goats are susceptible to both molecularly characterised ovine haemoplasmas. On the other hand, goat sucking lice (L. stenopsis) do not appear to be potential vectors of these agents.

scholarly journals Utility of Complete Mitochondrial Genomes in Phylogenetic Classification of the Species of Anopheles (Culicidae: Anophelinae)

2021 ◽  
Author(s):  
Taghi Ghassemi-Khademi ◽  
Mohammad Ali Oshaghi ◽  
Hassan Vatandoost ◽  
Seyed Massoud Madjdzadeh ◽  
Mohammad Amin Gorouhi
Keyword(s):  
Evolutionary Biology ◽  
Phylogenetic Trees ◽  
Complete Mitochondrial Genome ◽  
Malaria Vectors ◽  
Mitochondrial Genomes ◽  
Phylogenetic Classification ◽  
Anopheles Mosquitoes ◽  
Blood Sucking ◽  
Complete Mitochondrial Genomes

Background: Among the blood-sucking insects, Anopheles mosquitoes have a very special position, because they transmit parasites of the genus Plasmodium, which cause malaria as one of the main vector-borne disease worldwide. The aim of this review study was to evaluate utility of complete mitochondrial genomes in phylogenetic classification of the species of Anopheles. Methods: The complete mitochondrial genome sequences belonging to 28 species of the genus Anopheles (n=32) were downloaded from NCBI. The phylogenetic trees were constructed using the ML, NJ, ME, and Bayesian inference methods. Results: In general, the results of the present survey revealed that the complete mitochondrial genomes act very accu- rately in recognition of the taxonomic and phylogenetic status of these species and provide a higher level of support than those based on individual or partial mitochondrial genes so that by using them, we can meticulously reconstruct and modify Anopheles classification. Conclusion: Understanding the taxonomic position of Anopheles, can be a very effective step in better planning for controlling these malaria vectors in the world and will improve our knowledge of their evolutionary biology.

scholarly journals Variation of mitochondrial minichromosome composition in Hoplopleura lice (Phthiraptera: Hoplopleuridae) from rats

2020 ◽  
Author(s):  
Yi-Tian Fu ◽  
Yu Nie ◽  
De-Yong Duan ◽  
Guo-Hua Liu
Keyword(s):  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Coding Region ◽  
Illumina Platform ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Blood Sucking ◽  
The Family ◽  
Sucking Lice ◽  
Mt Genome

Abstract Background: The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice (Hoplopleura kitti and H. akanezumi) from this family, but some minichromosomes were unidentified in their mt genomes.Methods: We sequenced the mt genome of the rat louse Hoplopleura sp. with an Illumina platform and compared its mt genome organization with H. kitti and H. akanezumi.Results: Fragmented mt genome of the rat louse Hoplopleura sp. contains 37 genes which are on 12 circular mt minichromosomes. Each mt minichromosome is 1.8–2.7 kb long and contains 1–5 genes and one large non-coding region. The gene content and arrangement of mt minichromosomes of Hoplopleura sp. (n = 3) and H. kitti (n = 3) are different from those in H. akanezumi (n = 3). Phylogenetic analyses based on the deduced amino acid sequences of the eight protein-coding genes showed that the Hoplopleura sp. was more closely related to H. akanezumi than to H. kitti, and then they formed a monophyletic group.Conclusions: Comparison among the three rat lice revealed variation in the composition of mt minichromosomes within the genus Hoplopleura. Hoplopleura sp. is the first species from the family Hoplopleuridae for which a complete fragmented mt genome has been sequenced. The new data provide useful genetic markers for studying the population genetics, molecular systematics and phylogenetics of blood-sucking lice.

scholarly journals Variation in mitochondrial minichromosome composition among Hoplopleura lice (Phthiraptera: Hoplopleuridae) from rats

2020 ◽  
Author(s):  
Yi-Tian Fu ◽  
Yu Nie ◽  
De-Yong Duan ◽  
Guo-Hua Liu
Keyword(s):  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Coding Region ◽  
Illumina Hiseq ◽  
Protein Coding ◽  
Blood Sucking ◽  
The Family ◽  
Sucking Lice ◽  
Illumina Hiseq Platform ◽  
Mt Genome

Abstract Background The family Hoplopleuridae contains at least 183 species of blood-sucking lice, which widely parasitize both mice and rats. Fragmented mitochondrial (mt) genomes have been reported in two rat lice (Hoplopleura kitti and H. akanezumi) from this family, but some minichromosomes were unidentified in their mt genomes. Methods We sequenced the mt genome of rat louse Hoplopleura sp. with an Illumina Hiseq platform and compared its mt genome organization with H. kitti and H. akanezumi. Results Fragmented mt genome of the rat louse Hoplopleura sp. contains 37 genes which are on 12 circular mt minichromosomes. Each mt minichromosome is 1.8–2.7 kb long, which contains 1–5 genes and one large non-coding region. The gene content and arrangement of three mt minichromosomes of Hoplopleura sp. and H. kitti are different from that of the three mt minichromosomes of H. akanezumi. Phylogenetic analyses based on the deduced amino acid sequences of the eight protein-coding genes showed that the Hoplopleura sp. was more closely related to H. akanezumi than to H. kitti, and then they form a monophyletic group. Conclusions Comparison among the three rat lice revealed variation in the composition of mt minichromosomes within the genus Hoplopleura. Hoplopleura sp. is the first species from the family Hoplopleuridae for which a complete fragmented mt genome has been sequenced. The new data provides useful genetic markers for studying the population genetics, molecular systematics and phylogenetics of blood-sucking lice.

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