scholarly journals IgG antibody responses to Plasmodium falciparum merozoite antigens in Kenyan children have a short half-life

2007 ◽  
Vol 6 (1) ◽  
Author(s):  
Samson M Kinyanjui ◽  
David J Conway ◽  
David E Lanar ◽  
Kevin Marsh
Keyword(s):  
Plasmodium Falciparum ◽  
Half Life ◽  
Antibody Responses ◽  
Igg Antibody ◽  
Short Half Life ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

scholarly journals SEROPREVALENCE OF IMMUNOGLOBULIN IgG ANTIBODY RESPONSE TO PLASMODIUM FALCIPARUM MEROZOITE ANTIGENS AMONG CHILDREN IN MINNA, NORTH CENTRAL, NIGERIA

2021 ◽  
pp. 31-35
Author(s):  
YAMMAN-USMAN HADIJAH ◽  
OMALU CJ INNOCENT ◽  
ABUBAKAR ABDULKADIR ◽  
ABOLARINWA SO ◽  
EKE S SAMUEL ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antibody Response ◽  
Strong Relationship ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Cross Sectional ◽  
Significant Difference ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

Objective: A cross-sectional study was carried out in a representative cohort of children in Minna aged 6 months–17 years to determine the correlation between immunoglobulin G (IgG) antibody responses to Plasmodium falciparum merozoite antigens. Methods: Plasma samples from 93 children were exposed to Enzyme-Linked Immunosorbent Assay for the measurement of IgG antibody production against P. falciparum. Results: There was a high seroprevalence of IgG antibody against P. falciparum antigens tested with 74.20%. The seroprevalence for the male category was quite higher as compared with that of the female category, though, analysis using Mann–Whitney U-test revealed IgG antibody response to P. falciparum infection in the male was significantly different as compared to the female category (p<0.05). Furthermore, the prevalence of IgG antibody against P. falciparum antigen increased with age, with the lowest observed in 6 months–5 years 66.66%. Kruskal–Wallis H test showed a non-significant difference in the production of IgG antibody against P. falciparum antigen between different cohorts, and no correlation exists between them (p>0.05). An evidence of more than 50% was found for the production of IgG antibody by sub-microscopic parasite. On the other hand, microscopically positive P. falciparum samples recorded more seroprevalence of 68.81% as against negative samples, though significant difference between the negative and positive P. falciparum infected samples and the production of IgG antibody was not observed (p>0.05). Conclusion: This study has demonstrated a boosting immune responses by sub-microscopic parasite and also suggests a strong relationship between production of IgG antibody and malaria transmission, rather than protective immunity.

scholarly journals Measuring antibody avidity to Plasmodium falciparum merozoite antigens using a multiplex immunoassay approach

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Diane Wallace Taylor ◽  
Naveen Bobbili ◽  
Alex Kayatani ◽  
Samuel Tassi Yunga ◽  
Winifrida Kidima ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Antibody Avidity ◽  
Multiplex Immunoassay ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

Systematic analysis of natural antibody responses to P. falciparum merozoite antigens by protein arrays

2013 ◽  
Vol 78 ◽  
pp. 148-158 ◽  
Author(s):  
Yan-Ting Fan ◽  
Yue Wang ◽  
Chuan Ju ◽  
Ting Zhang ◽  
Bin Xu ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Natural Antibody ◽  
Protein Arrays ◽  
Systematic Analysis ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

scholarly journals Immunoglobulin G Subclass-Specific Responses against Plasmodium falciparum Merozoite Antigens Are Associated with Control of Parasitemia and Protection from Symptomatic Illness

2009 ◽  
Vol 77 (3) ◽  
pp. 1165-1174 ◽  
Author(s):  
Danielle I. Stanisic ◽  
Jack S. Richards ◽  
Fiona J. McCallum ◽  
Pascal Michon ◽  
Christopher L. King ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Vaccine Development ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Igg Subclass ◽  
Apical Membrane Antigen 1 ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

ABSTRACT Substantial evidence indicates that antibodies to Plasmodium falciparum merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria antigens induce distinct immunoglobulin G (IgG) subclass responses, but the importance of different responses in protective immunity from malaria is not known and the factors determining subclass responses in vivo are poorly understood. We examined IgG and IgG subclass responses to the merozoite antigens MSP1-19 (the 19-kDa C-terminal region of merozoite surface protein 1), MSP2 (merozoite surface protein 2), and AMA-1 (apical membrane antigen 1), including different polymorphic variants of these antigens, in a longitudinal cohort of children in Papua New Guinea. IgG1 and IgG3 were the predominant subclasses of antibodies to each antigen, and all antibody responses increased in association with age and exposure without evidence of increasing polarization toward one subclass. The profiles of IgG subclasses differed somewhat for different alleles of MSP2 but not for different variants of AMA-1. Individuals did not appear to have a propensity to make a specific subclass response irrespective of the antigen. Instead, data suggest that subclass responses to each antigen are generated independently among individuals and that antigen properties, rather than host factors, are the major determinants of IgG subclass responses. High levels of AMA-1-specific IgG3 and MSP1-19-specific IgG1 were strongly predictive of a reduced risk of symptomatic malaria and high-density P. falciparum infections. However, no antibody response was significantly associated with protection from parasitization per se. Our findings have major implications for understanding human immunity and for malaria vaccine development and evaluation.

scholarly journals Analysis of Antibodies to Newly Described Plasmodium falciparum Merozoite Antigens Supports MSPDBL2 as a Predicted Target of Naturally Acquired Immunity

2013 ◽  
Vol 81 (10) ◽  
pp. 3835-3842 ◽  
Author(s):  
Kevin K. A. Tetteh ◽  
Faith H. A. Osier ◽  
Ali Salanti ◽  
Gathoni Kamuyu ◽  
Laura Drought ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Risk Ratio ◽  
Statistical Significance ◽  
Clinical Malaria ◽  
Content Type ◽  
Type Antigen ◽  
Falciparum Merozoite ◽  
Merozoite Antigens ◽  
Allelic Type

ABSTRACTProspective studies continue to identify malaria parasite genes with particular patterns of polymorphism which indicate they may be under immune selection, and the encoded proteins require investigation. Sixteen new recombinant protein reagents were designed to characterize three such polymorphic proteins expressed inPlasmodium falciparumschizonts and merozoites: MSPDBL1 (also termed MSP3.4) and MSPDBL2 (MSP3.8), which possess Duffy binding-like (DBL) domains, and SURFIN4.2, encoded by a member of the surface-associated interspersed (surf) multigene family. After testing the antigenicities of these reagents by murine immunization and parasite immunofluorescence, we analyzed naturally acquired antibody responses to the antigens in two cohorts in coastal Kenya in which the parasite was endemic (Chonyi [n= 497] and Ngerenya [n= 461]). As expected, the prevalence and levels of serum antibodies increased with age. We then investigated correlations with subsequent risk of clinical malaria among children <11 years of age during 6 months follow-up surveillance. Antibodies to the polymorphic central region of MSPDBL2 were associated with reduced risk of malaria in both cohorts, with statistical significance remaining for the 3D7 allelic type after adjustment for individuals' ages in years and antibody reactivity to whole-schizont extract (Chonyi, risk ratio, 0.51, and 95% confidence interval [CI], 0.28 to 0.93; Ngerenya, risk ratio, 0.38, and 95% CI, 0.18 to 0.82). For the MSPDBL1 Palo Alto allelic-type antigen, there was a protective association in one cohort (Ngerenya, risk ratio, 0.53, and 95% CI, 0.32 to 0.89), whereas the other antigens showed no protective associations after adjustment. These findings support the prediction that antibodies to the polymorphic region of MSPDBL2 contribute to protective immunity.

scholarly journals Distinct kinetics in antibody responses to 111 Plasmodium falciparum antigens identifies novel serological markers of recent malaria exposure

2020 ◽  
Author(s):  
Victor Yman ◽  
James Tuju ◽  
Michael T White ◽  
Gathoni Kamuyu ◽  
Kennedy Mwai ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Disease Burden ◽  
Antibody Responses ◽  
Serological Markers ◽  
Limited Data ◽  
Malaria Surveillance ◽  
Igg Antibody ◽  
Malaria Exposure ◽  
C And N ◽  
Antibody Kinetics

Strengthening malaria surveillance is a key intervention needed to reduce the global disease burden. Reliable serological markers of recent malaria exposure could dramatically improve current surveillance methods by allowing for accurate estimates of infection incidence from limited data. We studied the IgG antibody response to 111 Plasmodium falciparum proteins in travellers followed longitudinally after a natural malaria infection in complete absence of re-exposure. We identified a novel combination of five serological markers (GAMA, MSP1, MSPDBL1 C- and N-terminal, and PfSEA1) that detect exposure within the previous 3-months with >80% sensitivity and specificity. Using mathematical modelling, we examined the antibody kinetics and determined that responses informative of recent exposure display several distinct characteristics: rapid initial boosting and decay, less inter-individual variation in response kinetics, and minimal persistence over time. These serological exposure markers can be incorporated into routine malaria surveillance to guide efforts for malaria control and elimination.

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