scholarly journals Molecular characterization and phylogenetic analysis of small ruminant lentiviruses isolated from Canadian sheep and goats

2011 ◽  
Vol 8 (1) ◽  
Author(s):  
Yvan L'Homme ◽  
Mourad Ouardani ◽  
Valérie Lévesque ◽  
Giuseppe Bertoni ◽  
Carole Simard ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Small Ruminant ◽  
Sheep And Goats ◽  
Small Ruminant Lentiviruses

scholarly journals A51 Genetic variability of small ruminant lentiviruses in sheep and goats from single-species flocks from Poland

2019 ◽  
Vol 5 (Supplement_1) ◽  
Author(s):  
Monika Olech ◽  
Jacek Kuźmak
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variability ◽  
Phylogenetic Trees ◽  
Small Ruminant ◽  
Phylogenetic Analyses ◽  
Single Species ◽  
Genetic Distances ◽  
Sheep And Goats ◽  
Viral Recombination ◽  
Species Flocks

Abstract Previous phylogenetic analyses of small ruminant lentivirus (SRLV) sequences found in Poland revealed the circulation of subtype A1 in both sheep and goats, subtypes B1 in goats, and subtypes B2, A12, and A13 in sheep only. This study aimed to analyze the genetic nature of SRLV circulating in sheep and goats from single-species flocks. In order to analyze the degree of genetic variability, the fragments of gag and env genes of 24 SRLV strains were amplified by PCR, cloned into plasmid vectors, sequenced, and consensus sequences were aligned to each other and to reference sequences available from GenBank. Phylogenetic analysis was performed using the Geneious tree-builder tool, and phylogenetic trees were constructed using Mr Bayes (using the general time reversible substitution model) within Geneious Pro 5.3. Pairwise genetic distances were calculated in MEGA 6. Phylogenetic analysis revealed that the strains were highly heterogeneous and represented ovine strains belonging to subtypes A12 and B2 and caprine strains grouped in subtypes B1, B2, A1, and A12. In addition, two novel subtypes, A16 and A17, were found in goats. The mean pairwise genetic distances of gag and env sequences of both clusters were above 15 per cent nucleotide divergence when compared to all other subtypes within group A, which is a criterion required to distinguish a new subtype. Additionally, the existence of two separated clusters was confirmed by high bootstrap values. Co-infections with strains belonging to different subtypes within A and B groups were detected in one sheep and four goats originating from four flocks. Since the co-infection with more than one lentivirus genotype offers an opportunity for viral recombination, the possible recombination events were tested based on RDP analysis. For all co-infected animals, no evidence of recombination was found within the gag gene; however, env sequences showed some recombination patterns in three samples. In conclusion, we have demonstrated extended genetic variability of SRLV in sheep and goats from Poland with the existence of co-infection and recombination events.

Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes

2019 ◽  
Vol 177 ◽  
pp. 160-166 ◽  
Author(s):  
Dalva Alana Aragão de Azevedo ◽  
Jomar Patrício Monteiro ◽  
Raymundo Rizaldo Pinheiro ◽  
Mauricio de Alvarenga Mudadu ◽  
Alice Andrioli ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Small Ruminant ◽  
Small Ruminant Lentiviruses

Serotyping versus genotyping in infected sheep and goats with small ruminant lentiviruses

2021 ◽  
Vol 252 ◽  
pp. 108931
Author(s):  
Gabriel Eduardo Acevedo Jiménez ◽  
Jorge Luis Tórtora Pérez ◽  
Cecilia Rodríguez Murillo ◽  
Beatriz Arellano Reynoso ◽  
Hugo Ramírez Álvarez
Keyword(s):  
Small Ruminant ◽  
Infected Sheep ◽  
Sheep And Goats ◽  
Small Ruminant Lentiviruses

Comparison of two PCR and one ELISA techniques for the detection of small ruminant lentiviruses (SRLVs) in milk of sheep and goats

2013 ◽  
Vol 94 (3) ◽  
pp. 817-819 ◽  
Author(s):  
N. Barquero ◽  
A. Domenech ◽  
A. Arjona ◽  
J.F. Fernández-Garayzabal ◽  
J.A. Ruiz-Santa-Quiteria ◽  
...  
Keyword(s):  
Small Ruminant ◽  
Sheep And Goats ◽  
Small Ruminant Lentiviruses

scholarly journals Genotyping Based on the LTR Region of Small Ruminant Lentiviruses from Naturally Infected Sheep and Goats from Mexico

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Wolfang P. S. Mendiola ◽  
Jorge L. Tórtora ◽  
Humberto A. Martínez ◽  
María M. García ◽  
Sandra Cuevas-Romero ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Transcription Factor ◽  
Binding Sites ◽  
Small Ruminant ◽  
Sequence Data ◽  
Competitive Elisa ◽  
Infected Sheep ◽  
Tata Box ◽  
Function Loss ◽  
Small Ruminant Lentiviruses

Small ruminant lentiviruses (SRLVs) belong to the genusLentivirusin the Retroviridae family. There are five genotypes (A, B, C, D, and E), where genotypes A and B have a global distribution and genotypes C, D, and E are limited to Europe. The presence of SRLV has been confirmed in Mexico, with genotype B detected in the central region of the country. We examined the presence of SRLVs and genotype prevalence in 1014 sheep and 1383 goats from 12 Mexican states. Using a commercial competitive ELISA (cELISA) test, we detected SRLV antibodies in 107 sheep (10.55%) and 466 goats (33.69%). We used an endpoint PCR to amplify the LTR region on seropositive animals. A total of 50 sheep and 75 goats tested positive via PCR. Positive amplicons from 11 sheep and 17 goats from ten Mexican States were cloned and sequenced. With the LTR sequence data obtained in this study, a phylogenetic analysis was performed; we also constructed a phylogenetic tree using the obtained sequences and GenBank’s available sequences. All studied sequences were associated with genotype B, specifically with the FESC-752 isolate previously identified in Mexico. Highly conserved transcription factor binding sites were observed in analyzed alignments, such as AML (vis), AP-4, and TATA box. However, we identified nucleotide differences at site AP-1 that suggest function loss. Our study found that ovine and caprine genotype B SRLVs are widely distributed in Mexico; a highly conserved LTR region among the sequences evaluated in this study was also found.

scholarly journals Molecular Characterization of Small Ruminant Lentiviruses in Polish Mixed Flocks Supports Evidence of Cross Species Transmission, Dual Infection, a Recombination Event, and Reveals the Existence of New Subtypes within Group A

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2529
Author(s):  
Monika Olech ◽  
Jacek Kuźmak
Keyword(s):  
Small Ruminant ◽  
Dual Infection ◽  
Surface Glycoprotein ◽  
Tata Box ◽  
Sheep And Goats ◽  
R Region ◽  
Complex Picture ◽  
Group A ◽  
Small Ruminant Lentiviruses

Small ruminant lentiviruses (SRLVs) are a group of highly divergent viruses responsible for global infection in sheep and goats. In a previous study we showed that SRLV strains found in mixed flocks in Poland belonged to subtype A13 and A18, but this study was restricted only to the few flocks from Małopolska region. The present work aimed at extending earlier findings with the analysis of SRLVs in mixed flocks including larger numbers of animals and flocks from different part of Poland. On the basis of gag and env sequences, Polish SRLVs were assigned to the subtypes B2, A5, A12, and A17. Furthermore, the existence of a new subtypes, tentatively designed as A23 and A24, were described for the first time. Subtypes A5 and A17 were only found in goats, subtype A24 has been detected only in sheep while subtypes A12, A23, and B2 have been found in both sheep and goats. Co-infection with strains belonging to different subtypes was evidenced in three sheep and two goats originating from two flocks. Furthermore, three putative recombination events were identified within gag and env SRLVs sequences derived from three sheep. Amino acid (aa) sequences of immunodominant epitopes in CA protein were well conserved while Major Homology Region (MHR) had more alteration showing unique mutations in sequences of subtypes A5 and A17. In contrast, aa sequences of surface glycoprotein exhibited higher variability confirming type-specific variation in the SU5 epitope. The number of potential N-linked glycosylation sites (PNGS) ranged from 3 to 6 in respective sequences and were located in different positions. The analysis of LTR sequences revealed that sequences corresponding to the TATA box, AP-4, AML-vis, and polyadenylation signal (poly A) were quite conserved, while considerable alteration was observed in AP-1 sites. Interestingly, our results revealed that all sequences belonging to subtype A17 had unique substitution T to A in the fifth position of TATA box and did not have a 11 nt deletion in the R region which was noted in other sequences from Poland. These data revealed a complex picture of SRLVs population with ovine and caprine strains belonging to group A and B. We present strong and multiple evidence of dually infected sheep and goats in mixed flocks and present evidence that these viruses can recombine in vivo.

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