Optimization of L-asparaginase production by Serratia marcescens (NCIM 2919) under solid state fermentation using coconut oil cake

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

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Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

Nepal Journal of Biotechnology ◽

10.3126/njb.v2i1.5641 ◽

1970 ◽

Vol 2 (1) ◽

pp. 26-36 ◽

Cited By ~ 1

Author(s):

Parvatham Madhu Latha ◽

Pallem Chanakya ◽

Manipati Srikanth

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Maximum Yield ◽

Initial Moisture Content ◽

Coconut Oil ◽

Malt Extract ◽

Fermentation Time ◽

Aspergillus Fischeri ◽

Fermentation Parameters ◽

Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36

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Aspergillus niger Str 3 and Neurospora sitophila for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/439/1/012020 ◽

2020 ◽

Vol 439 ◽

pp. 012020

Author(s):

A Kanti ◽

I Idris ◽

I M Sudiana

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Coconut Oil ◽

Phytase Production ◽

Coconut Oil Cake

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Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

Biotechnology Research International ◽

10.1155/2014/674286 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

B. M. Vastrad ◽

S. E. Neelagund

Keyword(s):

Solid State ◽

Ammonium Nitrate ◽

Ammonium Chloride ◽

Solid State Fermentation ◽

Sodium Nitrate ◽

Coconut Oil ◽

Medium Composition ◽

Significant Variable ◽

Streptomyces Fradiae ◽

Coconut Oil Cake

Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R2) was 0.9232, which ensures an acceptable admissibility of the model.

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Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes

Nature Environment and Pollution Technology ◽

10.46488/nept.2021.v20i03.020 ◽

2021 ◽

Vol 20 (3) ◽

Author(s):

J. Mary Sheela ◽

K. Divya ◽

S. Premina

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Coconut Oil ◽

Industrial Wastes ◽

Submerged Cultivation ◽

Brewery Wastewater ◽

Groundnut Oil ◽

Penicillium Species ◽

Amylase Production ◽

Coconut Oil Cake

Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS).

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Isolation and characterization of three distinct forms of lipases from Candida rugosa produced in solid state fermentation

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132001000200016 ◽

2001 ◽

Vol 44 (2) ◽

pp. 213-221 ◽

Cited By ~ 34

Author(s):

Sailas Benjamin ◽

Ashok Pandey

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Specific Activity ◽

Candida Rugosa ◽

Gel Filtration ◽

Coconut Oil ◽

Apparent Molecular Weight ◽

Ammonium Sulphate Precipitation ◽

Isolation And Characterization ◽

Ultra Filtration

Three distinct forms (Lip A, Lip B and Lip C) of extra-cellular lipases (EC- 3.1.1.3), produced by Candida rugosa in solid state fermentation (SSF) were purified and characterised. SSF was carried out in glass columns using coconut oil cake and wheat bran. The enzyme was purified from the aqueous extract of fermented matter by ammonium sulphate precipitation, dialysis, ultra-filtration and gel filtration using Sephadex-200 to a 43-fold purification and 64.35-mg/ml specific activity. SDS-PAGE of purified enzyme revealed three distinct bands, indicating the existence of three iso-forms, Lip A, Lip B and Lip C with apparent molecular weight about 64,000, 62,000 and 60,000 Da, respectively. All the three iso-forms were optimally active at 35-40°C and pH 7-8. They showed marked differences in their Km values with different saturated and unsaturated triacyl glycerols. Ag++ and Hg++ strongly inhibited enzyme activity of all the iso-forms, Mn++ has no effect and Ca++ and Mg++ enhanced the activity. EDTA also strongly inhibited the enzyme activities of iso-forms. However, activities of all the three lipases were completely inhibited by serine protease inhibitors such as 3,4-dichloroisocoumarin, pefabloc and partially by phenylmethanesulphonyl fluoride. To the best of our knowledge, this is the first report describing the purification and characterisation of C. rugosa lipase iso-forms from solid cultures. These lipase iso-forms with diverse characteristics produced in solid cultures may find potential application in biomedical field.

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