scholarly journals Aspergillus niger Str 3 and Neurospora sitophila for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation

2020 ◽  
Vol 439 ◽  
pp. 012020
Author(s):  
A Kanti ◽  
I Idris ◽  
I M Sudiana
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Phytase Production ◽  
Coconut Oil Cake

scholarly journals Enhanced phytase production by Aspergillus niger mutants in solid state fermentation

2021 ◽  
Author(s):  
Shahzad Mahmood ◽  
◽  
Memuna G. Shahid ◽  
Muhammad Nadeem ◽  
Rubina Nelofer ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Research Work ◽  
Wild Strain ◽  
Chemical Mutagenesis ◽  
Poultry Feed ◽  
Control Group ◽  
Phytase Production

The present research work was conducted to improve the phytase production by genetic alteration of Aspergillus niger with induced mutagenesis using solid state fermentation. Strain improvement was carried out in the presence of ultra violet (UV) irradiation and ethylmethane sulphonate (EMS) [0.5% v/v] treatments for various time intervals. We reported an improved strain of Aspergillus niger designated as UV-3 mutant producing a zone of hydrolysis of about 40 mm, in comparison to wild strain (26 mm). The highest enzyme activity was found to be 547.64 IU/g for UV-3 mutant followed by EMS-4 mutant (492.23 IU/g)compared to wild strain which showed 406.45 IU/g of enzyme activity. There was 1.35 fold increase in phytase production after mutation studies of Aspergillus niger. Phytase was applied as poultry feed additive and given to broiler chickens for 5 weeks. The results exhibited that there was increase in body weight gain (BWG) of chicks for experimental group (2028 g) in comparison to control group (1903 g). Thus, physical and chemical mutagenesis was proved as an effective technique for the improvement of strain and ultimately for enhanced and economical phytase production for different industrial applications.

scholarly journals Amylase Production by Aspergillus niger and Penicillium Species by Solid-State and Submerged Cultivation Using Two Food Industrial Wastes

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
J. Mary Sheela ◽  
K. Divya ◽  
S. Premina
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Coconut Oil ◽  
Industrial Wastes ◽  
Submerged Cultivation ◽  
Brewery Wastewater ◽  
Groundnut Oil ◽  
Penicillium Species ◽  
Amylase Production ◽  
Coconut Oil Cake

Amylase enzymes are starch degrading enzymes and have received a great deal of attention due to their perceived technology importance and economic benefit. Amylase enzymes are considered important enzymes used in starch processing industries for the hydrolysis of polysaccharides like starch into simple sugar constituents. This enzyme is also involved in the commercial production of glucose. Solid-state cultivation and submerged cultivation have tremendous potentials for enzyme amylase production by using different solid substrates like rice bran, wheat bran, coconut oil cake, and groundnut oil cake which are rich in starch. These agro-industrial wastes are considered cheap raw materials for the production of amylase. Wastewater from the industry like brewery can also be used as a liquid substrate for submerged cultivation. It may have the possibility of depurination of wastewater. In the present study, Aspergillus niger and Penicillium species were isolated and their amylase activity was determined by the starch hydrolysis method. Enzyme production was done by using coconut oil cake as a substrate for solid-state fermentation and brewery wastewater as a substrate for submerged fermentation. The enzyme produced by the organisms was extracted and enzyme assay was done by the Dinitrisalicilic method (DNS method). The protein estimation was done by Lowry Folin’s method. The qualitative assay was carried out by performing Gas Chromatography-Mass Spectroscopy (GC-MS).

scholarly journals Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues

2010 ◽  
Vol 4 ◽  
pp. 16-25 ◽  
Author(s):  
Vasudeo Zambare
Keyword(s):  
Solid State ◽  
Aspergillus Oryzae ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Groundnut Oil ◽  
Amylolytic Enzyme ◽  
Coconut Oil Cake ◽  
Agro Residues ◽  
Glucoamylase Production

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

scholarly journals Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

1970 ◽  
Vol 2 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Parvatham Madhu Latha ◽  
Pallem Chanakya ◽  
Manipati Srikanth
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Coconut Oil ◽  
Malt Extract ◽  
Fermentation Time ◽  
Aspergillus Fischeri ◽  
Fermentation Parameters ◽  
Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36 

scholarly journals Phytase Production byAspergillus nigerCFR 335 andAspergillus ficuumSGA 01 through Submerged and Solid-State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Gunashree B. Shivanna ◽  
Govindarajulu Venkateswaran
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Enzyme Level ◽  
Solid Substrate ◽  
Aspergillus Ficuum ◽  
Phytase Production ◽  
Potato Dextrose Broth ◽  
Substrate Medium

Fermentation is one of the industrially important processes for the development of microbial metabolites that has immense applications in various fields. This has prompted to employ fermentation as a major technique in the production of phytase from microbial source. In this study, a comparison was made between submerged (SmF) and solid-state fermentations (SSF) for the production of phytase fromAspergillus nigerCFR 335 andAspergillus ficuumSGA 01. It was found that both the fungi were capable of producing maximum phytase on 5th day of incubation in both submerged and solid-state fermentation media.Aspergillus nigerCFR 335 andA. ficuumproduced a maximum of 60.6 U/gds and 38 U/gds of the enzyme, respectively, in wheat bran solid substrate medium. Enhancement in the enzyme level (76 and 50.7 U/gds) was found when grown in a combined solid substrate medium comprising wheat bran, rice bran, and groundnut cake in the ratio of 2 : 1 : 1. A maximum of 9.6 and 8.2 U/mL of enzyme activity was observed in SmF byA. nigerCFR 335 andA.ficuum, respectively, when grown in potato dextrose broth.

scholarly journals Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
B. M. Vastrad ◽  
S. E. Neelagund
Keyword(s):  
Solid State ◽  
Ammonium Nitrate ◽  
Ammonium Chloride ◽  
Solid State Fermentation ◽  
Sodium Nitrate ◽  
Coconut Oil ◽  
Medium Composition ◽  
Significant Variable ◽  
Streptomyces Fradiae ◽  
Coconut Oil Cake

Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R2) was 0.9232, which ensures an acceptable admissibility of the model.

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