scholarly journals Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

1970 ◽  
Vol 2 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Parvatham Madhu Latha ◽  
Pallem Chanakya ◽  
Manipati Srikanth
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Coconut Oil ◽  
Malt Extract ◽  
Fermentation Time ◽  
Aspergillus Fischeri ◽  
Fermentation Parameters ◽  
Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36 

scholarly journals Production of L-glutaminase From Agro-industrial Waste by Halomonas Elongata MM-5 Under Solid State Fermentation

2021 ◽  
Author(s):  
Mohammed Mujahed ◽  
Dhiraj G Jirgale ◽  
Basavraj M Kareppa
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Nitrogen Sources ◽  
Malt Extract ◽  
Inoculum Level ◽  
Bengal Gram ◽  
Halomonas Elongata

Abstract In this study L-glutaminase production by extremely halotolerant Halomonas elongata MM-5 using solid-state fermentation was investigated. Screening of a variety of agro-industrial byproducts such as rice husk, green gram husk, bengal gram husk, red gram husk, safflower oil cake, groundnut oil cake, black gram husk, groundnut skin and wheat bran was carried out individually and in different combinations. Optimization of various physicochemical parameters namely incubation time, pH, temperature, initial moisture content, carbon sources, nitrogen sources, L-glutamine concentration and inoculum level was carried out. Among the various substrates screened individually red gram husk supported the maximum production of enzyme 79.03±0.49 IU/gds. In the mixture of substrates screened red gram husk and bengal gram husk in the ratio (60:40) showed maximum 92.06±1.42 IU/gds enzyme production. The L-glutaminase production was maximum after 4 days of incubation period, pH 8, temperature 40°C and 80 percent moisture content. The lactose and malt extract used as carbon and nitrogen sources respectively supported the maximum yield of L-glutaminase. After screening and optimization of various parameters, the yield of L-glutaminase increased from 79.03 to 159.12 IU/gds.

scholarly journals Lovastatin Production byAspergillus terreusUsing Agro-Biomass as Substrate in Solid State Fermentation

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Mohammad Faseleh Jahromi ◽  
Juan Boo Liang ◽  
Yin Wan Ho ◽  
Rosfarizan Mohamad ◽  
Yong Meng Goh ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Moisture Content ◽  
Nitrogen Source ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Initial Moisture Content ◽  
Fermentation Time ◽  
Maximum Production ◽  
Additional Nitrogen

Ability of two strains ofAspergillus terreus(ATCC 74135 and ATCC 20542) for production of lovastatin in solid state fermentation (SSF) using rice straw (RS) and oil palm frond (OPF) was investigated. Results showed that RS is a better substrate for production of lovastatin in SSF. Maximum production of lovastatin has been obtained usingA. terreusATCC 74135 and RS as substrate without additional nitrogen source (157.07 mg/kg dry matter (DM)). Although additional nitrogen source has no benefit effect on enhancing the lovastatin production using RS substrate, it improved the lovastatin production using OPF with maximum production of 70.17 and 63.76 mg/kg DM forA. terreusATCC 20542 andA. terreusATCC 74135, respectively (soybean meal as nitrogen source). Incubation temperature, moisture content, and particle size had shown significant effect on lovastatin production (P<0.01) and inoculums size and pH had no significant effect on lovastatin production (P>0.05). Results also have shown that pH 6, 25°C incubation temperature, 1.4 to 2 mm particle size, 50% initial moisture content, and 8 days fermentation time are the best conditions for lovastatin production in SSF. Maximum production of lovastatin using optimized condition was 175.85 and 260.85 mg/kg DM forA. terreusATCC 20542 and ATCC 74135, respectively, using RS as substrate.

Production and Characterization of Amylases by Aspergillus niger under Solid State Fermentation Using Agro Industrials Products

2006 ◽  
Vol 2 (3) ◽  
Author(s):  
Michele Rigon Spier ◽  
Adenise Lorenci Woiciechowski ◽  
Luciana Porto de Souza Vandenberghe ◽  
Carlos Ricardo Soccol
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Sugar Cane ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Cassava Starch ◽  
Sugar Cane Bagasse ◽  
Production Costs

The use of agro industrials products cassava starch and sugar cane bagasse present a great potential as substrate and support, respectively, showing low production costs for amylases production under solid-state fermentation by Aspergillus niger LPB 28. The effect of various factors was examined and some variables were optimized to ?-amylase and amyloglucosidase production. Inoculum rate in different concentrations (105, 106, 107 and 108 spores/g of cassava starch) were used to inoculate the solid substrate for the SSF. The material was supplemented with nitrogen sources; calcium source and micronutrients solution. The ratios of cassava starch to sugar cane bagasse (1/1; 1.5/1; 1/1.5; 1/2; 2/1; 1/3; 3/1) were tested and the initial moisture content varying 80, 85 and 90%. Cultivation was carried out at temperatures 25, 30 and 35ºC for 48, 60 and 72 h. Study of influence of pH initial in SSF was conduced in pH 4.0, 5.0, 6.0, 7.0 and 8.0. Statistical analysis was carried out using the software STATISTICA 5.1 (StatSoft, Tulsa, OK, USA). A 32 complete factorial design (2 factors, 3 levels and 9 experiments) was used to study the effect of the factors in ?-amylase and amyloglucosidase production. The results showed cassava starch and sugar cane bagasse at the ratios of 2/1 was the best for optimum production of ?-amylase and amyloglucosidase. The maximum yield (?-amylase 1732.95 U/g of cassava starch and amyloglucosidase 2044.94 U/g of cassava starch) was achieved with optimized process parameters such as incubation period (60 hours), moisture level was 90%, inoculation rate (105 spores/g of dried material), pH 4.0 and fermentation temperature (30ºC). The nitrogen source (KNO3) also enhanced the ?-amylase and amyloglucosidase activities. In kinetic characterization of enzymes the Michaelis-Menten constant KM and maximum velocity Vmax (KM 10.84 g/l, Vmax 3.42 g/l) for ?-amylase and KM 11.32 g/l and Vmax 5.18 g/l for amyloglucosidase.

scholarly journals Solid State Fermentation of Aspergillus oryzae for Glucoamylase Production on Agro residues

2010 ◽  
Vol 4 ◽  
pp. 16-25 ◽  
Author(s):  
Vasudeo Zambare
Keyword(s):  
Solid State ◽  
Aspergillus Oryzae ◽  
Wheat Bran ◽  
Solid State Fermentation ◽  
Coconut Oil ◽  
Groundnut Oil ◽  
Amylolytic Enzyme ◽  
Coconut Oil Cake ◽  
Agro Residues ◽  
Glucoamylase Production

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

scholarly journals Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
B. M. Vastrad ◽  
S. E. Neelagund
Keyword(s):  
Solid State ◽  
Ammonium Nitrate ◽  
Ammonium Chloride ◽  
Solid State Fermentation ◽  
Sodium Nitrate ◽  
Coconut Oil ◽  
Medium Composition ◽  
Significant Variable ◽  
Streptomyces Fradiae ◽  
Coconut Oil Cake

Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R2) was 0.9232, which ensures an acceptable admissibility of the model.

Optimization of Fermentation Conditions for Higher Monacolin K Production by Monascus purpureus

2013 ◽  
Vol 781-784 ◽  
pp. 1397-1402 ◽  
Author(s):  
Li Juan Yu ◽  
Hong Xing Zhang ◽  
Yuan Hong Xie ◽  
Si Ming Ma ◽  
Hui Liu ◽  
...  
Keyword(s):  
Solid State ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Monascus Purpureus ◽  
Monacolin K ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Orthogonal Experiments ◽  
Optimization Of Fermentation ◽  
Optimized Conditions

Monacolin K is a secondary metabolite of Monascus fermentation with lowering blood fat, cholesterol and blood sugar. The culture substrates and fermentation conditions were optimized by means of L9 (33) orthogonal experiments. The optimized fermentation conditions were studied and concluded: initial moisture content 50%, material thickness 4 cm and fermentation time 13 days. Using rice as substrate, the maximum yield of monacolin K was 2.50 mg/g in the optimized conditions. Using glutinousrice as substrate, the maximum yield of monacolin K was 2.71 mg/g in the optimized conditions. The founding determined that glutinousrice was used as substrate in solid state fermentation by Monascus purpureus to produce monacolinK.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

Sign in / Sign up

Export Citation Format

Share Document