Use of Coconut Oil Cake for the Production of α- Amyalse Using Aspergillus oryzae by Solid State Fermentation

Glucoamylase is a well recognized amylolytic enzyme used in food industry, which is generally produced by Aspergillus genus under solid-state fermentation (SSF). This study presents production of glucoamylase by Aspergillus oryzae on the solid surface of rice husk, wheat bran, rice bran, cotton seed powder, corn steep solids, bagasse powder, coconut oil cake, and groundnut oil cake as substrates. Optimization of the SSF media and parameters resulted in a 24% increase in the glucoamylase activity. Optimum glucoamylase production (1986 μmoles of glucose produced per minute per gram of dry fermented substrate) was observed on wheat bran supplemented with 1%, (w/w) starch, 0.25%, (w/w) urea at pH 6, 100%, (v/w) initial moisture and 30°C after incubation 120 hrs. Therefore, A. oryzae can be useful in bioprocessing application for saccharification of agro-residues. Keywords: Glucoamylase, Aspergillus oryzae, solid state fermentation, agro residues DOI: 10.3126/ijls.v4i0.2892 International Journal of Life Sciences Vol.4 2010 pp.16-25

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Lovastatin production by Aspergillus fischeri under solid state fermentation from coconut oil cake

Nepal Journal of Biotechnology ◽

10.3126/njb.v2i1.5641 ◽

1970 ◽

Vol 2 (1) ◽

pp. 26-36 ◽

Cited By ~ 1

Author(s):

Parvatham Madhu Latha ◽

Pallem Chanakya ◽

Manipati Srikanth

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Maximum Yield ◽

Initial Moisture Content ◽

Coconut Oil ◽

Malt Extract ◽

Fermentation Time ◽

Aspergillus Fischeri ◽

Fermentation Parameters ◽

Coconut Oil Cake

The main aim of the present investigation was to optimize the fermentation parameters that enhance the maximum production of lovastatin by Aspergillus fischeri using coconut oil cake as the solid substrate under solid state fermentation. The maximum yield of lovastatin (14.77 mg/g dry substrate) using coconut oil cake as the substrate was achieved with the following optimized process parameters: fermentation time (7 days), initial moisture content (60% v/w), inoculum volume (2ml of five day old culture), initial pH (5.0), incubation temperature (30ºC), lactose (1% w/v) and malt extract (1% w/v).Keywords: Lovastatin; Aspergillus fischeri; Coconut oil cake; Fermentation parameters; OptimizationDOI: http://dx.doi.org/10.3126/njb.v2i1.5641 Nepal Journal of Biotechnology Jan.2012, Vol.2(1): 26-36

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Aspergillus niger Str 3 and Neurospora sitophila for phytase production on coconut oil cake supplemented with rice brand in solid-state fermentation

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/439/1/012020 ◽

2020 ◽

Vol 439 ◽

pp. 012020

Author(s):

A Kanti ◽

I Idris ◽

I M Sudiana

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Coconut Oil ◽

Phytase Production ◽

Coconut Oil Cake

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Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

Biotechnology Research International ◽

10.1155/2014/674286 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

B. M. Vastrad ◽

S. E. Neelagund

Keyword(s):

Solid State ◽

Ammonium Nitrate ◽

Ammonium Chloride ◽

Solid State Fermentation ◽

Sodium Nitrate ◽

Coconut Oil ◽

Medium Composition ◽

Significant Variable ◽

Streptomyces Fradiae ◽

Coconut Oil Cake

Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R2) was 0.9232, which ensures an acceptable admissibility of the model.

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