scholarly journals Demonstration of Bovine Viral Diarrhoea Virus Antigens in Cell Cultures and in Paraffin-embedded Tissue Sections by the Peroxidase-antiperoxidase (PAP) Technique Using Monoclonal Antibodies

1989 ◽  
Vol 30 (2) ◽  
pp. 231-233 ◽  
Author(s):  
Katinka Belák ◽  
E. J. Gimeno ◽  
S. Belák
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Cultures ◽  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Tissue Sections ◽  
Diarrhoea Virus ◽  
Virus Antigens

Epitopic characterisation of Turkish bovine viral diarrhoea virus (bvdv) isolates using monoclonal antibodies

2003 ◽  
Vol 51 (2) ◽  
pp. 237-244 ◽  
Author(s):  
K. Yeşilbağ ◽  
I. Burgu
Keyword(s):  
Monoclonal Antibodies ◽  
Plaque Assay ◽  
Bovine Viral Diarrhoea Virus ◽  
The Other ◽  
Bovine Viral Diarrhoea ◽  
Time Intervals ◽  
Field Isolates ◽  
Epitope Analysis ◽  
Diarrhoea Virus ◽  
Limiting Dilution

In this study, 15 bovine viral diarrhoea viruses (BVDV) isolated from the field in Turkey were characterised for their biotype, cloned and eventually analysed for their epitopic composition in terms of glycoprotein E2. Immunoplaque assay, plaque assay, limiting dilution and streptavidin-biotin-peroxidase techniques were used for biotype characterisation, cloning of cytopathic (cp) and noncytopathic (ncp) biotypes and epitope analysis, respectively. While 14 out of 15 BVDV isolates were distinguished as ncp biotype, 1 isolate was found to be containing both biotypes (cp + ncp). According to the reactivity patterns of isolates with 15 monoclonal antibodies, 4 different antigenic groups could be formed. There were no antigenic differences between the isolates derived from the same animal with various time intervals. On the other hand, biotype clones isolated from the same animal exhibited difference in one epitope. This is the first study describing antigenic characterisation of BVDV field isolates in Turkey.

scholarly journals ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies

1990 ◽  
Vol 23 (1-4) ◽  
pp. 193-201 ◽  
Author(s):  
C. Lecomte ◽  
J.J. Pin ◽  
L. De Moerlooze ◽  
D. Vandenbergh ◽  
A.F. Lambert ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Viral Diarrhoea Virus ◽  
Recombinant Antigen ◽  
Bovine Viral Diarrhoea ◽  
Specific Antibodies ◽  
Diarrhoea Virus
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