Epitopic characterisation of Turkish bovine viral diarrhoea virus (bvdv) isolates using monoclonal antibodies

2003 ◽  
Vol 51 (2) ◽  
pp. 237-244 ◽  
Author(s):  
K. Yeşilbağ ◽  
I. Burgu
Keyword(s):  
Monoclonal Antibodies ◽  
Plaque Assay ◽  
Bovine Viral Diarrhoea Virus ◽  
The Other ◽  
Bovine Viral Diarrhoea ◽  
Time Intervals ◽  
Field Isolates ◽  
Epitope Analysis ◽  
Diarrhoea Virus ◽  
Limiting Dilution

In this study, 15 bovine viral diarrhoea viruses (BVDV) isolated from the field in Turkey were characterised for their biotype, cloned and eventually analysed for their epitopic composition in terms of glycoprotein E2. Immunoplaque assay, plaque assay, limiting dilution and streptavidin-biotin-peroxidase techniques were used for biotype characterisation, cloning of cytopathic (cp) and noncytopathic (ncp) biotypes and epitope analysis, respectively. While 14 out of 15 BVDV isolates were distinguished as ncp biotype, 1 isolate was found to be containing both biotypes (cp + ncp). According to the reactivity patterns of isolates with 15 monoclonal antibodies, 4 different antigenic groups could be formed. There were no antigenic differences between the isolates derived from the same animal with various time intervals. On the other hand, biotype clones isolated from the same animal exhibited difference in one epitope. This is the first study describing antigenic characterisation of BVDV field isolates in Turkey.

scholarly journals Serological survey of wild cervids in England and Wales for bovine viral diarrhoea virus

2020 ◽  
Vol 187 (7) ◽  
pp. e47-e47
Author(s):  
Jo Hardstaff ◽  
Hannah Hunt ◽  
Laura Tugwell ◽  
Carole Thomas ◽  
Laila Elattar ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
The Other ◽  
Bovine Viral Diarrhoea ◽  
Serum Samples ◽  
East Anglia ◽  
England And Wales ◽  
Source Of Infection ◽  
Diarrhoea Virus ◽  
Cattle Herds ◽  
Wild Deer

BackgroundBovine viral diarrhoea (BVD) is a production disease commonly found in British cattle herds. Species other than cattle have been shown to be infected with the virus, thereby providing a potential source of infection for livestock. This study surveyed serum samples taken from 596 culled wild deer from England and Wales, between 2009 and 2010, for the presence of BVD antibodies.Methods596 samples were tested with the SVANOVIR BVDV p80-Ab ELISA and a subset of 64 were tested with the IDEXX BVDV p80-Ab ELISA. ELISA results were confirmed using serum neutralisation tests.Results2/596 samples (0.35 per cent) tested positive for BVD antibodies using the Svanova test and one of these tested positive and the other inconclusive using the IDEXX test; both were confirmed positive with serum neutralisation tests. These were both red deer stags, one from Devon and the other from East Anglia.ConclusionsThe results indicate that it is unlikely that BVD virus is widely circulating within the wild deer population and particularly unlikely that persistently infected deer are present in the populations surveyed. These results suggest that wild deer are unlikely to be a significant reservoir of BVD infection in cattle.

scholarly journals ELISA detection of bovine viral diarrhoea virus specific antibodies using recombinant antigen and monoclonal antibodies

1990 ◽  
Vol 23 (1-4) ◽  
pp. 193-201 ◽  
Author(s):  
C. Lecomte ◽  
J.J. Pin ◽  
L. De Moerlooze ◽  
D. Vandenbergh ◽  
A.F. Lambert ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Viral Diarrhoea Virus ◽  
Recombinant Antigen ◽  
Bovine Viral Diarrhoea ◽  
Specific Antibodies ◽  
Diarrhoea Virus

scholarly journals BVDV alters uterine prostaglandin production during pregnancy recognition in cows

Reproduction ◽  
2016 ◽  
Vol 151 (6) ◽  
pp. 605-614 ◽  
Author(s):  
Zhangrui Cheng ◽  
Ayimuguli Abudureyimu ◽  
Chike F Oguejiofor ◽  
Rebekah Ellis ◽  
Amy Teresa Barry ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Bovine Viral Diarrhoea Virus ◽  
The Other ◽  
Uterine Disease ◽  
Bovine Viral Diarrhoea ◽  
Endometrial Cells ◽  
Diarrhoea Virus ◽  
Pregnancy Recognition ◽  
Reproductive Processes

AbstractEmbryonic mortality in cows is at least in part caused by failure of pregnancy recognition (PR). Evidence has shown that bovine viral diarrhoea virus (BVDV) infection can disrupt pregnancy. Prostaglandins (PG) play important roles in many reproductive processes, such as implantation. The aim of this study was to investigate the effect of BVDV infection on uterine PG production and PR using anin vitroPR model. Bovine uterine endometrial cells isolated from ten BVDV-free cows were cultured and treated with 0 or 100ng/mL interferon-τ (IFNT) in the absence or presence of non-cytopathic BVDV (ncpBVDV). PGF2αand PGE2concentrations in the spent medium were measured using radioimmunoassays, and in the treated cells expression of the genes associated with PG production and signalling was quantified using qPCR. The results showed that the IFNT challenge significantly stimulatedPTGS1andPTGER3mRNA expression and PGE2production; however, these stimulatory effects were neutralised in the presence of ncpBVDV infection. ncpBVDV infection significantly increasedPTGS1andmPGES1mRNA expression and decreasedAKR1B1expression, leading to increased PGE2and decreased PGF2αconcentrations and an increased PGE2:PGF2αratio. The other tested genes, includingPGR,ESR1,OXTR,PTGS2,PTGER2andPTGFR, were not significantly altered by IFNT, ncpBVDV or their combination. Our study suggests that BVDV infection may impair PR by (1) inhibiting the effect of IFNT on uterine PG production and (2) inducing an endocrine switch of PG production from PGF2αto PGE2to decrease uterine immunity, thereby predisposing the animals to uterine disease.

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