Analysis of Soluble Bovine Viral Diarrhoea Virus Antigens and Serological Relationship to Virus Structural Glycoproteins

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

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Anti-Bovine Viral Diarrhoea Virus Antibodies in Adult Zambian Patients Infected with the Human Immunodeficiency Virus

International Journal of STD & AIDS ◽

10.1177/095646249300400510 ◽

1993 ◽

Vol 4 (5) ◽

pp. 300-302 ◽

Cited By ~ 5

Author(s):

M Giangaspero ◽

G Vacirca ◽

D Morgan ◽

K S Baboo ◽

N P Luo ◽

...

Keyword(s):

Synergistic Effects ◽

Bovine Viral Diarrhoea Virus ◽

Hiv Infections ◽

Chronic Diarrhoea ◽

Bovine Viral Diarrhoea ◽

Diarrhoea Virus ◽

Immunodeficiency Virus ◽

Mechanism Of Interaction ◽

Asymptomatic Individuals ◽

Hiv Seropositive

Bovine viral diarrhoea (BVD) virus is a cosmopolitan pestivirus of animals which is associated with diarrhoea, immunosuppression and synergy with other pathogens. This study was conducted to establish the prevalence of anti-BVD virus antibodies in healthy Zambian adults and those with asymptomatic and symptomatic HIV disease. Sera from 1159 adults were tested for anti-BVD virus antibodies using the indirect immunofluorescence test and the confirmatory Western blot. Of the 1159 sera examined, 180 (15.5%) showed significantly elevated titres of anti-BVD antibodies. These included 70 out of 477 (14.7%) HIV-negative healthy adults; 73 out of 480 (15.2%) of HIV-positive asymptomatic individuals; 23 out of 129 (17.8%) HIV-seropositive patients with associated illnesses excluding diarrhoea; and 14 out of 73 (19.2%) of HIV-seropositive patients with chronic diarrhoea. HIV-seropositive patients with chronic diarrhoea or associated illnesses appear to have significantly increased seroprevalence of anti-BVD virus antibodies ( P = >0.01). The mechanism of interaction between BVD virus and HIV infections and the synergistic effects with other opportunistic pathogens in humans requires definition.

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